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- 저자 : Yulin Fang (검색결과 2 건)
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Comparison of long-term behavior between prestressed concrete and corrugated steel web bridges
Yulin Zhan,Fang Liu,Zhongguo John Ma,Zhiqiang Zhang,Zengqiang Duan,Ruinian Song 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.30 No.6
Prestressed concrete (PC) bridges using corrugated steel webbing have emerged as one of the most promising forms of steel-concrete composite bridge. However, their long-term behavior is not well understood, especially in the case of largespan bridges. In order to study the time-dependent performance, a large three-span PC bridge with corrugated steel webbing was compared to a similar conventional PC bridge to examine their respective time-dependent characteristics. In addition, a threedimensional finite element method with step-by-step time integration that takes into account cantilever construction procedures was used to predict long-term behaviors such as deflection, stress distribution and prestressing loss. These predictions were based upon four well-established empirical creep prediction models. PC bridges with a corrugated steel web were observed to have a better long-term performance relative to conventional PC bridges. In particular, it is noted that the pre-cambering for PC bridges with a corrugated steel web could be smaller than that of conventional PC bridges. The ratio of side-to-mid span has great influence on the long-term deformation of PC bridges with a corrugated steel web, and it is suggested that the design value should be between 0.4 and 0.6. However, the different creep prediction models still showed a weak homogeneity, thus, the further experimental research and the development of health monitoring systems are required to further progress our understanding of the long-term behavior of PC bridges with corrugated steel webbing.
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Identification and expression analysis of ERECTA family genes in grape (Vitis vinifera L.)
Min Liu,Wanping Li,Zhuo Min,Xianghan Cheng,Yulin Fang 한국유전학회 2019 Genes & Genomics Vol.41 No.6
Background ERECTA family (ERf) genes are found in many dicots and monocots, and play important roles in plant developmental processes and stress responses. However, there is little known on ERf genes in grape (Vitis vinifera L.). Objectives The primary objective of this study was to identify the ERf genes in grape, and to analyze their expression profiles in different organs, during development, and in response to hormone treatments and abiotic/biotic stresses. Methods ERf protein sequences of dicots were aligned in the grape genome (V. vinifera cv. Pinot Noir, PN40024, 12X) with Blast server. The locus tags obtained were inputted in NCBI to retrieve corresponding nucleotide and protein accession numbers. The subcellular localization experiment was performed by the transient expression of VvERECTA-GFP and VvERL2-GFP in mesophyll protoplasts of Arabidopsis. The expression levels of ERf genes in grape leaves were detected by qRT-PCR after hormone treatments and abiotic/biotic stresses. Results We first identified the ERf genes in the grape genome, including VvERECTA and VvERL2. Their cDNA full-length sequences were obtained with the accession numbers MG601756 and MG601757. The result of subcellular localization indicated that the fusion proteins of VvERECTA and VvERL2 were localized in the plasma membrane. There were four conserved domains identified in VvERECTA and VvERL2, including a LRRNT-2, a LRR, a transmembrane and a protein kinase domain. The grape ERf genes expressed highly in young aboveground organs. As grape leaves or berries becoming mature, VvERECTA expressed in a declining trend. The transcript abundance of VvERL2 decreased during leaves development, but showed no significant differences during berries development. The hormone treatments of ABA, SA, MeJA and BR could induce the expression of VvERECTA and VvERL2. The treatments of heat, drought, downy and powdery mildew significantly increased the expression levels of the grape ERf genes. Conclusion The grape ERECTA gene family might play crucial roles in response to abiotic and biotic stresses. We provide the first description of the grape ERf genes and the most comprehensive analysis of their expressions in different biological processes.
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