상지 보조 소프트로봇의 의복화를 위한 디자인 개발 프로세스

홍유화 ( Yuhwa Hong ),박주연 ( Juyeon Park ),남윤자 ( Yun Ja Nam ),박대근 ( Daegeun Park ),조규진 ( Kyu-jin Cho ),김연주 ( Youn Joo Kim ) 한국의류산업학회 2021 한국의류산업학회지 Vol.23 No.1

This study proposes a design process for an upper limb assistive wearable soft robot that will enable the development of a clothing product for an upper limb assistive soft robot. A soft robot made of a flexible and soft material that compensates for the shortcomings of existing upper limb muscle strength assistive devices is being developed. Consequently, a clothing process of the upper limb assistive soft robot is required to increase the possibility of wearing such a device. The design process of the upper limb auxiliary soft robot is presented as follows. User analysis and required performance deduction-Soft robot design-upper limb assistive wearable soft robot prototype design and production-evaluation. After designing the clothing according to the design process, the design was revised and supplemented repeatedly according to the results of the clothing evaluation. In the post-production evaluation stage, the first and second prototypes were attached to actual subjects, and the second prototype showed better results. The developed soft robot evaluated if the functionality as a clothing function and the functionality as the utility of the device were harmonized. The convergence study utilized a process of reducing friction conducted through an understanding and cooperation between research fields. The results of this study can be used as basic data to establish the direction of prototype development in fusion research.

Expression of PCK1 gene in skeletal muscle cells using muscle-specific promoter

Bon Chul Koo,Mo Sun Kwon,Yuhwa Nam,Teoan Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

To test the muscle cell specific gene expression, we examined the ability of human α-skeletal muscle actin (ACTA) promoter or human myoglobin (hMb) promoter to direct the expression of the GFP gene in both muscle and non-muscle cells, respectively. C2C12 cells, a mouse myoblast cell line, provide a powerful model to study skeletal muscle differentiation in vitro. We intended to use this cell line as a model for skeletal muscle-specific gene expression during myogenic differentiation from myoblast to myotubes. We compared marker gene expression profiles of proliferating and differentiated C2C12 cells using RT-PCR and fluorescent microscopy analysis. Also, we found that the expression of PCK1 gene under the control of ACTA promoter was proportionally increased as C2C12 differentiated into myotube form. PCK1 is involved in the regulation of gluconeogenesis. In previous research, transgenic mice with overexpressing PCK1 in skeletal muscle showed a greatly enhanced level of physical activity, which extends well into old age. This is due, in part, to an increased number of mitochondria and a high concentration of triglyceride in their skeletal muscles. These mice also had very little body fat, despite eating 60% more than controls. We also constructed a mesenchymal stem cell line and fetal fibroblast cell line for the experiments aiming to make transgenic animals in which the PCK1 gene is specifically expressed in muscle tissue. Accumulated knowledge of this approach could be applicable to a variety of related biological areas including transgenic animal research, gene function study, anti-aging study, etc. This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Export Promotion Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (316002-5).

Expression of Recombinant hEPO in the Egg White of Transgenic Chickens

Bon Chul Koo,Mo Sun Kwon,Dohyang Kim,Yuhwa Nam,Teoan Kim 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

In this study, we report successful expression of recombinant human erythropoietin (hEPO) in the egg white of transgenic hens using a feline immunodeficiency virus (FIV)- based lentiviral vector as an exogenous gene deliverer. hEPO is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. FIV vectors permit high levels of transgene expression in quails and chickens. We constructed a FIV vector containing a hEPO cDNA sequence driven by an oviduct-specific ovalbumin promoter, and microinjected into the subgerminal cavity of stage X chick embryos to generate transgenic chicken. Out of 208 injected eggs, 10 chicks were hatched after 21 days of incubation, and one of the G0 hatched chicken expressed the vector-encoded hEPO gene in sperm. Successful germline transmission of the transgene was also confirmed in G1 transgenic chicks produced from crossing G0 transgenic roosters with non-transgenic hens. One rooster was mated to wild-type hens to produce 518 G1 progeny. PCR analysis of blood samples from these progeny revealed that there were four G1 transgenic offspring, corresponding to a 0.77% germline transmission rate. Subsequently, Southern blot analysis of the genomic DNA from four G1 transgenic chickens was carried out to verify the stable genomic integration and copy number of the transgene in the genome. Quantitative analyses of the blood and egg white samples taken from G1 transgenic chickens resulted in 4,810~6,600 IU/mL (40.1~55.0 ㎍/mL) of hEPO in egg white, whereas 18~25 mIU/mL (0.15~0.2 ng/mL) in serum. The biological activity of the recombinant hEPO in egg white was comparable to its commercially available counterpart. We conclude that successful expression of recombinant hEPO in the egg white of transgenic chickens implies an important step towards efficient production of human cytokine from the transgenic animal bioreactor.

Production of Germline Transgenic Chickens Expressing High levels of Recombinant hEPO using a MoMLV-based Retrovirus Vector

Bon Chul Koo,Mo Sun Kwon,Dohyang Kim,Sanga Kim,Museog Choe,Yuhwa Nam,Teoan Kim 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

Expression of Active Human Interferon Alpha 2b gene nder the Control of Ovalbumin Promoter in Transgenic Chickens

Bon Chul Koo,Mo Sun Kwon,Dohyang Kim,Sanga Kim,Museog Choe,Yuhwa Nam,Teoan Kim 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

Expression of Active Human Interferon Alpha 2b gene nder the Control of Ovalbumin Promoter in Transgenic Chickens

Bon Chul Koo,Mo Sun Kwon,Dohyang Kim,Sanga Kim,Museog Choe,Yuhwa Nam,Teoan Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

Human interferon alpha 2b (hIFNα-2b) is an important immune regulator widely used in clinic, for the treatment of chronic hepatitis, hairy cell leukemia, chronic myelogenous leukemia and multiple myeloma, etc. The clinically used hIFNα-2b is generally produced by E. Coli, which lacks the post-translational O-glycosylation of naturally synthesized protein, and has a short serum half-life. In this study, we report the successful generation of transgenic chickens that produce hIFNα-2b in the egg white using a feline immunodeficiency virus (FIV)-based lentiviral vector. In preliminary in vitro study, the hIFNα-2b gene under the control of CMV promoter expressed as much as 2,650 ng/㎖ in CEF-LNC-hIFNα-2bW cell. A FIV vector packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected underneath the blastoderm of freshly laid chicken eggs (stage X) to produce a hIFNα -2b transgenic chicken. Out of 187 injected eggs, 55 chicks were hatched after 21 days of incubation, and 27 of the G0 hatched chicks expressed the vector-encoded hIFNα-2b gene. The expression of recombinant hIFNα-2b in transgenic chickens constitutes an important step towards low-cost and full biological activity production of this protein drug in bioreactor. This work was supported by the Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea, and by a grant from the Next-Generation BioGreen 21 Program (No. PJ011178), Rural Development Administration, Republic of Korea.

Production of Germline Transgenic Chickens Expressing High levels of Recombinant hEPO using a MoMLV-based Retrovirus Vector

Bon Chul Koo,Mo Sun Kwon,Dohyang Kim,Sanga Kim,Museog Choe,Yuhwa Nam,Teoan Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of hEPO. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). One rooster was mated to wild-type hens to produce 748 G1 progeny. PCR analysis of blood samples from these progeny revealed that there were seven G1 transgenic offspring, corresponding to a 0.9% germline transmission rate. Subsequently, Southern blot analysis of the genomic DNA from three G1 transgenic chickens was carried out to verify the stable genomic integration and copy number of the transgene in the genome. Quantitative analyses of the blood samples taken from G1 transgenic chickens resulted in 4,150 ~ 10,823 IU/㎖ (34.6 ~ 90.2 ㎍/㎖) of hEPO in the blood. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. Red blood cell numbers were more than three-fold higher in the transgenic chickens compared to the non-transgenic chickens. Successful germline transmission of the transgene was also confirmed in G2 transgenic chicks produced from crossing G1 transgenic roosters with non-transgenic hens. We confirmed that 13 transgenic chicks of 45 G2 progeny, corresponding to a 28.9% germline transmission rate. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors. This work was supported by the Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea, and by a grant from the Next-Generation BioGreen 21 Program (No. PJ011178), Rural Development Administration, Republic of Korea.