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        Electrolyte solution transport in electropolar nanotubes

        Zhao, Jianbing,Culligan, Patricia J,Qiao, Yu,Zhou, Qulan,Li, Yibing,Tak, Moonho,Park, Taehyo,Chen, Xi IOP Pub 2010 Journal of Physics, Condensed Matter Vol.22 No.31

        <P>Electrolyte transport in nanochannels plays an important role in a number of emerging areas. Using non-equilibrium molecular dynamics (NEMD) simulations, the fundamental transport behavior of an electrolyte/water solution in a confined model nanoenvironment is systematically investigated by varying the nanochannel dimension, solid phase, electrolyte phase, ion concentration and transport rate. It is found that the shear resistance encountered by the nanofluid strongly depends on these material/system parameters; furthermore, several effects are coupled. The mechanisms of the nanofluidic transport characteristics are explained by considering the unique molecular/ion structure formed inside the nanochannel. The lower shear resistance observed in some of the systems studies could be beneficial for nanoconductors, while the higher shear resistance (or higher effective viscosity) observed in other systems might enhance the performance of energy dissipation devices.</P>

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        Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase from Penicillium oxalicum sp. 68

        Zhou, Tong,Hu, Yanbo,Yan, Xuecui,Cui, Jing,Wang, Yibing,Luo, Feng,Yuan, Ye,Yu, Zhenxiang,Zhou, Yifa The Korean Society for Microbiology and Biotechnol 2022 Journal of microbiology and biotechnology Vol.32 No.8

        Arabinogalactans have diverse biological properties and can be used as pharmaceutical agents. Most arabinogalactans are composed of β-(1→3)-galactan, so it is particularly important to identify β-1,3-galactanases that can selectively degrade them. In this study, a novel exo-β-1,3-galactanase, named PoGal3, was screened from Penicillium oxalicum sp. 68, and hetero-expressed in P. pastoris GS115 as a soluble protein. PoGal3 belongs to glycoside hydrolase family 43 (GH43) and has a 1,356-bp gene length that encodes 451 amino acids residues. To study the enzymatic properties and substrate selectivity of PoGal3, β-1,3-galactan (AG-P-I) from larch wood arabinogalactan (LWAG) was prepared and characterized by HPLC and NMR. Using AG-P-I as substrate, purified PoGal3 exhibited an optimal pH of 5.0 and temperature of 40℃. We also discovered that Zn<sup>2+</sup> had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

      • Investigating the effects of non-persistent cracks’ parameters on the rock fragmentation mechanism underneath the U shape cutters using experimental tests and numerical simulations with PFC2D

        Jinwei Fu,Hadi Haeri,Vahab Sarfarazi,Sh. Mohamadi Bolban Abad,Mohammad Fatehi Marji,Gholamreza Saeedi,Yibing Yu 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.4

        This paper aims to study the fracture mechanism of rocks under the ‘u’ shape cutters considering the effects of crack (pre-existing crack) distances, crack spacing and crack inclination angles. The effects of loading rates on the rock fragmentation underneath these cutters have been also studied. For this purpose, nine experimental samples with dimensions of 5 cm×10 cm×10 cm consisting of the non-persistent cracks were prepared. The first three specimens’ sets had one non-persistent crack (pre-existing crack) with a length of 2 cm and angularity of 0°, 45°, and 90°. The spacing between the crack and the “u” shape cutter was 2 cm. The second three specimens” set had one non-persistent crack with a length of 2 cm and angularity of 0°, 45°, and 90° but the spacing between pre-existing crack and the “u” shape cutter was 4 cm. The third three specimens’ set has two non-persistent cracks with lengths of 2 cm and angularity of 0°, 45° and 90°. The spacing between the upper crack and the “u” shape cutter was 2 cm and the spacing between the lower crack and the upper crack was 2 cm. The samples were tested under a loading rate of 0.005 mm/s. concurrent with the experimental investigation. The numerical simulations were performed on the modeled samples with non-persistent cracks using PFC2D. These models were tested under three different loading rates of 0.005 mm/s, 0.01 mm/sec and 0.02 mm/sec. These results show that the crack number, crack spacing, crack angularity, and loading rate has important effects on the crack growth mechanism in the rocks underneath the “u” shape cutters. In addition, the failure modes and the fracture patterns in the experimental tests and numerical simulations are similar to one another showing the validity and accuracy of the current study.

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