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Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells
Park, Youngchan,Shin, Sangwon,Jin, Hyeonggyu,Park, Jiseong,Hong, Yeonki,Choi, Jaemin,Jung, Byunghyuck,Song, Hyunjoon,Seo, Daeha American Chemical Society 2018 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.140 No.45
<P>Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein-protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors.</P> [FIG OMISSION]</BR>