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Shi Jinping,Zhang Quanwei,Song Yali,Lei Zhaomin,Fu Lingjuan,Cheng Shuru 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.8
Objective: With improvements in living standards and increase in global population, the demand for meat products has been increasing; improved meat production from livestock could effectively meet this demand. In this study, we examined the differences in the muscle traits of different male crossbred sheep and attempted to identify key genes that regulate these traits. Methods: Dubo sheep×small-tailed Han sheep (DP×STH) and Suffolk×small-tailed Han sheep (SFK×STH) were selected to determine meat quality and production performance by Masson staining. Transcriptome sequencing and bioinformatic analysis were performed to identify differentially expressed genes (DEGs) related to meat quality. The presence of DEGs was confirmed by real-time polymerase chain reaction. Results: The production performance of SFK×STH sheep was better than that of DP×STH sheep, but the meat quality of DP×STH sheep was better than that of SFK×STH sheep. The muscle fiber diameter of DP×STH sheep was smaller than that of SFK×STH sheep. Twentytwo DEGs were identified. Among them, four gene ontology terms were related to muscle traits, and three DEGs were related to muscle or muscle fibers. There were no significant differences in the number of single nucleotide mutations and mutation sites in the different male parent cross combinations. Conclusion: This study provides genetic resources for future sheep muscle development and cross-breeding research. Objective: With improvements in living standards and increase in global population, the demand for meat products has been increasing; improved meat production from livestock could effectively meet this demand. In this study, we examined the differences in the muscle traits of different male crossbred sheep and attempted to identify key genes that regulate these traits.Methods: Dubo sheep×small-tailed Han sheep (DP×STH) and Suffolk×small-tailed Han sheep (SFK×STH) were selected to determine meat quality and production performance by Masson staining. Transcriptome sequencing and bioinformatic analysis were performed to identify differentially expressed genes (DEGs) related to meat quality. The presence of DEGs was confirmed by real-time polymerase chain reaction.Results: The production performance of SFK×STH sheep was better than that of DP×STH sheep, but the meat quality of DP×STH sheep was better than that of SFK×STH sheep. The muscle fiber diameter of DP×STH sheep was smaller than that of SFK×STH sheep. Twenty-two DEGs were identified. Among them, four gene ontology terms were related to muscle traits, and three DEGs were related to muscle or muscle fibers. There were no significant differences in the number of single nucleotide mutations and mutation sites in the different male parent cross combinations.Conclusion: This study provides genetic resources for future sheep muscle development and cross-breeding research.
SURFACE MODIFICATION AND CHARACTERIZATION OF Fe_3O_4/Au COMPOSITE NANOPARTICLES
FENG SHI,WENLI HUI,YALI CUI,CHAO CHEN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.2
Fe_3O_4/Au composite nanoparticles were modified with α-thio-ω-carboxy poly (ethylene glycol). Results from XRD, selected area diffraction, high-resolution TEM images, and dynamic lighting scattering illustrate that the particles have a core/shell composite structure and were monodispersed. Surface plasmon resonance measured by UV–Vis indicates that the absorption peak of the modified composite particle characteristic at 532 nm can be stably suspended in a different buffer. The modified composite particles also have good response to external magnetic field. The Fe_3O_4/Au composite nanoparticles are magnetically and optically active, and are useful for simultaneous magnetic and optical detection. Coupled with biomolecules, the advantages of these composite particles make them very promising for biomedical applications in the near future.
Li, Xiao,Shi, Xiaodan,Zou, Man,Luo, Yudi,Tan, Yali,Wu, Yexu,Chen, Lin,Li, Pei The Korean Society for Microbiology and Biotechnol 2015 한국미생물·생명공학회지 Vol.43 No.3
In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane-bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.
Congle Liu,Junxue Ren,Yali Zhang,Kaining Shi 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.6
As the performance potential of composite materials continues to be explored, CFRP has gradually become an important candidate material in various fields. However, CFRP is a typical difficult-to-process material because of its anisotropy and inhomogeneity. Inappropriate tool structure or parameters will lead to substandard surface quality. For this reason, a series of experiments are designed in this paper, including tool structure optimization experiment, milling parameter single factor experiment, and milling parameter orthogonal experiment. And because of the complexity of the composite surface, the surface roughness Ra commonly used in metal cutting is not used, but a series of 3D surface roughness indexes are used to evaluate the surface. The effect of process parameters on the processing quality was explored by single variable analysis, range analysis and grey relational analysis. It is concluded that better surface quality can be obtained by using multi-blade cutters with moderate helix angle. Furthermore, Medium feed per tooth is more beneficial to the surface quality. The small depth of cut can effectively reduce the milling force, but when the depth of cut is less than the thickness of CFRP single layer, the surface quality will decline sharply.
( Xiao Li ),( Xiaodan Shi ),( Man Zou ),( Yudi Luo ),( Yali Tan ),( Yexu Wu ),( Lin Chen ),( Pei Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2015 한국미생물·생명공학회지 Vol.43 No.3
In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane- bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.
The Disturbance Rejection Design Based on Physical Feedforward for Solid Oxide Fuel Cell
Zhenlong Wu,Wangying Shi,Donghai Li,Ting He,Yali Xue,Minfang Han,Song Zheng 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
The output voltage control of the Solid Oxide Fuel Cell (SOFC) is challenging due to the strong nonlinearity, modelling uncertainty, the dramatic change of the external current load and constraints on the amplitudes and change rates of the actuator. Besides, the desired range of the fuel utilization is another difficult for the control strategy. In this paper, Active disturbance rejection control (ADRC) is proposed to handle the nonlinearity, uncertainty and the external disturbance, and physical feedforward is designed to speed up the response of the whole system and keep the fuel utilization in the desired range. Instead of the SOFC model, the design of physical feedforward is based on the definition of fuel utilization which is featured by simplicity, generality and effectiveness. Simulation results show that the proposed control strategy has smaller variation range of the fuel utilization, shorter time of the changing process of the output voltage and better robustness than others control strategies.
Kai Liu,Xiaohang Zhang,Hao Wang,Xuwen He,Zhonghua Shangguan,Yali Shi,Shipeng Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5
Composite carbon-based nano-zero-valent iron materials not only have excellent characteristics of nano materials, but also solve the difficulty of recycling nano materials after use, and overcome the shortcomings of the nano materials. The introduction of copper increases the redox reaction activity of the micro-electrolytic material and expands the applicable pH range of the reaction environment. In the range of pH = 3–9, the removal efficiency of dichloromethanesulfonic acid (Cl2-MSA) was maintained at 95.5–99.7% after 30 min. Continuous dynamic simulation experiments verified that the composite carbon-based nano zero-valent iron material has a stable removal effect on the target pollutants.