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Kim, Young Hye,Marcus, Katrin,Grinberg, Lea Tenenholz,Goehler, Heike,Wiltfang, Jens,Stephan, Christian,Eisenacher, Martin,Hardt, Tanja,Martens, Lennart,J Dunn, Michael,Park, Young Mok,Meyer, Helmut E JOHN WILEY & SONS, LTD 2009 PROTEOMICS CLINICAL APPLICATIONS Vol.3 No.9
<P>The HUPO Brain Proteome Project (HUPO BPP) held its 11th workshop in Kolymbari on March 3, 2009. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in addition to the development of novel diagnostic techniques and new medications. The attendees came together to discuss sub-project progress in the clinical neuroproteomics of human or mouse models of Alzheimer's and Parkinson's disease, and to define the needs and guidelines required for more advanced proteomics approaches. With the election of new steering committees, the members of the HUPO BPP elaborated an actual plan promoting activities, outcomes, and future directions of the HUPO BPP to acquire new funding and new participants.</P>
Hamacher, Michael,Stephan, Christian,Eisenacher, Martin,Lewczuk, Piotr,Wiltfang, Jens,Martens, Lennart,Vizcaí,no, Juan Antonio,Kwon, Kyung-Hoon,Yoo, Jong Shin,Park, Young Mok,Beckers, Johannes,H WILEY-VCH Verlag 2007 Proteomics Vol.7 No.15
<P>The Wellcome Trust Conference Centre at Hinxton, UK, was the meeting place of the 7<SUP>th</SUP> HUPO Brain Proteome Project Workshop entitled “High Performance Proteomics”. It started on Wednesday, March 7, 2007 with a steering committee meeting followed by a two days series of talks dealing with the standardization and handling of tissues, body fluids as well as of proteomics data. The presentation and accompanying vivid discussions created a picture of actual strategies and standards in recent proteomics.</P>
Hamacher, Michael,Stephan, Christian,Hardt, Tanja,Eisenacher, Martin,Henkel, Andreas,Wiltfang, Jens,Jimenez, Connie R.,Park, Young Mok,Marcus, Katrin,Meyer, Helmut E. WILEY-VCH Verlag 2008 Proteomics Vol.8 No.9
<P>What are the current approaches in brain proteomics? Can we combine different, but complementary study designs to obtain better results concerning brain diseases? What are the neuro-hotspots, especially in Korea? These were some of the questions the participants of the 8<SUP>th</SUP> HUPO Brain Proteome Project Workshop tried to answer prior to the 6<SUP>th</SUP> HUPO World Congress in Seoul, Korea. Around 100 scientists came together during the afternoon of 7 October, 2007, to discuss and to catch up on the latest results and strategies concerning Huntington's disease, glioblastoma and standardization.</P>
Amir-Alexander Ghoniem,Yahya Aç,il,Jö,rg Wiltfang,Matthias Gierloff 대한해부학회 2015 Anatomy & Cell Biology Vol.48 No.2
To date there is no sufficient in vitro fat tissue engineering and a protocol has not been well established for this purpose. Therefore, we evaluated the in vitro influence of two different adipogenic growth media for their stimulation potential on different cell lineages to clearly define the most potent adipogenic growth media for future in vitro tissue engineering approaches. The samples for differentiation were composed of human adipogenic-derived stroma cells (hADSCs) and human bone marrow mesenchymal stroma cells (hMSCs). A normal adipogenic medium (NAM) and a specific adipogenic medium (SAM) were tested for their adipogenic stimulation potential. After 10 days and 21 days the relative gene expression was measured for the adipogenic marker genes PPARg2, C/EBPa, FABP4, LPL, and GLUT4 detected through real time reverse transcriptase polymease chain reaction (RT-PCR). Other study variables were the comparison between NAM and SAM and between the used cells hADSCs and hMSCs. Additionally an Oil-Red staining was performed after 21 days. Our results revealed that only SAM was significantly (P<0.05) superior in the differentiation process in contrast to NAM for 10 days and 21 days. As well was SAM superior to differentiate the used cell lineages. This was evaluated by the detected marker genes PPARg2, C/ EBPa, FABP4, LPL, and GLUT4 through real time RT-PCR and by Oil-Red staining. In addition, the hMSCs proofed to be equal donor cells for adipogenic differentiation especially when stimulated by SAM. The results suggest that the SAM should be established as a new standard medium for a more promising in vitro adipogenic differentiation.