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- 저자 : Wenfang Xu (검색결과 4 건)
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Expression, Purification, and Bioactivity of (GLP-1A2G)2-HSA Analogs in Pichia pastoris GS115
Wenfang Dou,Junshang Feng,Xiaomei Zhang,Hongyu Xu,Jinsong Shi,Zhenghong Xu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
We developed (GLP-1A2G)2-HSA (GGH) analogsthat are resistant to degradation and also show high serumglucose-reducing activity in vivo. Five analogs with Nterminalextensions were designed based on the proteinGGH. Next, we constructed recombinant plasmids capableof expressing the five analogs in methylotrophic yeastPichia pastoris GS115. Expression reached 150 mg/L in asmall-scale incubation. Fusion proteins were successfullypurified from the supernatant using ultrafiltration concentration,affinity absorption chromatography, hydrophobicchromatography, ion exchange chromatography and gelfiltration. A single band was observed on SDS-PAGE andthe purity was 97%. Activity test results suggested thatboth A-GGH and G-GGH showed better activity in vitroand that their cAMP levels were significantly increased by10-fold compared to GGH without N-terminal extension. Additionally, A-GGH efficiently enhanced the glucoseloweringeffect, which was maintained after the administrationfor 24 h. A-GGH is a potential drug for treating type 2diabetes.
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IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis
Wenfang Xu,Yanan Wang,Changzhong Jin,Weiyang Zhang,Jiangnan Chen,Xuefang Chen,Junli Gao,Junshun Gao,Hong Wang 대한면역학회 2023 Immune Network Vol.23 No.6
Autoimmune hepatitis (AIH) affects all age group and occurs mainly in women. Pyroptosis is a novel programmed cell death featured with cell bursting and release of proinflammatory cytokines. A deeper understanding of AIH pathogenesis will contribute to novel therapy for AIH patients. Here, we aimed to investigate the role of IL-17 in immune-mediated liver injury. The levels of cytokines were measured by ELISA, and mRNA levels of STAT3 and IFN gammainducible protein 16 (IFI16) were detected by PCR. Expressions of STAT3, IFI16, gasdermin D and cleaved caspase-1 were measured by western-blotting. Immunohistochemical staining and transmission electron microscopy were applied to evaluate liver histopathological changes of the treated mice. Our results showed that the levels of IFI16 was increased in hepatocytes treated with IL-17 protein, and further elevated after STAT3-overexpressed (STAT3-OE) lentivirus treatment. The levels of IFI16 were reduced in hepatocytes treated with IL-17 neutralizing Ab (nAb), but were significantly increased after STAT3-OE treatment. Pyroptosis was observed in hepatocytes treated with IL-17 protein, and further cell damage was observed after STAT3-OE lentivirus treatment. Liver damage was alleviated in mice treated with IL-17 nAb, however sever damage was experienced after STAT3-OE lentivirus treatment. A binding interaction between IFI16 and STAT3 was detected in IL-17 treated hepatocytes. Glutathione transaminase activity was enhanced in concanavalin A-induced AIH mice compared to the control group (p<0.01). IL-17 plays an important role in activating STAT3 and up-regulating IFI16, which may promote the pyroptosis in AIH-related liver injury through STAT3-IFI16 axis.
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Kang Wang,Wenfang Li,Wenzhi Xu,Shengyu Hou,Shaodong Hu 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11
The Aluminum–Silicon (Al–Si) die-casting alloys, such as the commercial A356 alloy, are expected to be used in heat-sinkand the device with high thermal conductivity due to their high production efficiency of casting process. These fields alsorequired them to possess sound mechanical properties. To meet these demands, the Strontium (Sr) was often utilized tomodify the silicon phase. According to our current work, the secondary dendrite arm spacing of the α-Al grains prominentlywas decreased when 0.05%–0.25% strontium was added. The decrease of the secondary dendrite arm spacing (SDAS) lead tothe enhancement of the mechanical strength as well as the improvement of the thermal and electrical conductivity. When theSr dosage was 0.15% in the commercial A356 alloy, the strength and the thermal conductivity of the A356 alloy simultaneouslyreached the maximum value. The improvement of the electrical and thermal conductivity might by contributed by theformation of a good conductor, Al2Si2Srphase, on the Si surface. Further investigations suggested that the improvement ofthermal conductivity was mainly due to the modification effect of Sr on the eutectic Si phase, which enlarged the specificarea between the α-Al/eutectic Si interface. The WDS analysis indicated that the solubility of Si decreased in aluminummatrix by increasing in the Sr dosage. However, the excessive dosage of strontium would result in the coarsening of themodified silicon phase, deteriorating the strength and the thermal and electrical conductivity of the modified A356 alloys.
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Hui Li,Zhenzhen Fu,Heng Li,Wenfang Dou,Jinsong Shi,Zhenghong Xu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
This study investigated the effects of hydroxylase cyptochrome P450 inducers on the efficiency of the biotransformation of dehydroepiandrosterone (DHEA) to 3β, 7α, 15α-trihydroxy-5-androsten-17-one (7α,15α-diOHDHEA)by Colletotrichum lini ST-1. Special attention was given to the substrate DHEA being the best inducer and the fact that it could improve the yield of 7α, 15α-diOHDHEA. Based on the effects of the DHEA pre-induction phases and additional concentrations on 7α, 15α-diOHDHEA production, a substrate pre-induction process was established as follows: 2 g/L DHEA was added for the first time after 12 h of inoculation, followed by the second addition of 6 g/L DHEA after 12 h later. The results showed that this substrate pre-induction process improved the content of cytochrome P450 and that the 7α, 15α-diOH-DHEA yield reached 90.1%, which was 26.9%higher than that obtained in the original process.
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