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RISS 인기검색어
- 검색키워드
- 저자 : Usha Rani Pathipati (검색결과 2 건)
- 무료
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Pathipati Usha Rani,Sambangi Pratyusha 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.2
The responses of the cotton plant, Gossypium hirsutum L. to herbivory by Spodoptera litura F. was studied in various laboratory experiments as a measure to understand the defense strategies of certain plant metabolites. Insect feeding damage enhanced the concentration of total phenol content and proteins, whereas amount of carbohydrates and amino acids were reduced. The experiments on estimation of anti-oxidative enzymes revealed stimulation in peroxidase, catalase, and superoxide dismutase levels and reduction in polyphenol oxidase and phenylanine ammonia lyase levels, signifying their defensive role in the plant. Specific phenolic acid changes were further carried out using High Performance Liquid Chromatography (HPLC),and distinct elevations in the gallic acid, catechin, and caffeic acid levels were observed in the infested cotton plant. Feeding assays towards S. litura with these phenolic compounds revealed activated detoxifying enzymes including β-glucosidase, carboxyl esterase, and glutathione-S-transferase in the insect gut indicating the toxicity. The aim of present study is to aid in further use of these specific phenolic acids towards effective management of the cotton pest, S. litura
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Sandhyarani Kurra,Usha Rani Pathipati 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
Behavioral responses of whitefly, Trialeurodes ricini's parasitoid, Encarsia formosa to induced plant surface chemicals due to T. ricini infestation of castor, Ricinus communis (L.) plants were studied. The impact of whitefly infestation in the form of quantitative and qualitative changes in the leaf biochemical constituents was also analyzed. The results of Y-tube olfactometer assays performed to investigate the role of pest induced plant semiochemicals in locating host plants by E. formosa showed that whitefly induced plants were not attractive to conspecifics and that the crude surface extracts of infested plants were attractive to the parasitoid when tested against extracts of uninfested plants. Hexane extract of whiteflies was more attractive to E. formosa when tested against surface chemicals of infested plants. The total free amino acids, proteins and the secondary metabolites such as phenols enhanced in whitefly fed plants. On the other hand, total carbohydrate content was reduced in whitefly infested castor leaves over healthy ones. Enzyme analyses after whitefly infestation showed that pest feeding significantly increased the activities of foliar phenyl alanine ammonia lyase (PAL), poly phenol oxidase (PPO) and catalase (CAT). A decrease in superoxide dismutase (SOD) and peroxidase (POD) enzyme activities was evident in pest infested plants. The individual phenolic acids from pest fed plants were identified using high performance liquid chromatography (HPLC) and comparedwith normal, intact plants. Hence it is concluded that pest infested plants exhibited defense strategies which may be exploited as a tool in the Integrated Pest Management for crop protection.
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