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Miyamoto, Tomofumi,Yamamoto, Atsushi,Sakai, Maki,Tanaka, Hiroyuki,Shoyama, Yukihiro,Higuchi, Ryuichi The Korean Society for Marine Biotechnology 2006 한국해양바이오학회지 Vol.1 No.4
In this study, we establish a thin-layer chromatography (TLC) immunostaining method for detecting starfish gangliosides. A new monoclonal antibody (MAb) against AG-2, the major gangliosides molecular species of Acanthaster planci, was produced by fusing hybridoma with splenocytes immunized to liposomal AG-2. BALB/c male mice were injected with liposomal AG-2 antigen, and immunized. Their splenocytos were isolated and fused with hypoxanthine-aminopterine-thimidine (HAT)-sensitive mouse myeloma cells. Hybridomas producing MAb reactive to AG-2 were cloned using the limited dilution method. Established hybridomas were cultured in eRDF medium. Crude MAb produced from clone 8D4 was purified with a magnesium pyrophosphate column. Enzyme immunoassay and TLC immunostaining of AG-2 were performed using the purified MAb. Structurally related gangliosides did not cross-react with anti-AG-2 antibodies. The detection limit of TLC immunostaining was 50 ng of AG-2. The newly established immunostaining method was further developed for detecting AG-2 distribution and qualitative analysis in tissues and/or organs. Our results show that the majority of AG-2 is present in the stomach of male A. planci, while AG-2 is distributed not only in the stomach but also in the the pyloric caeca of female A. planci.
Kubo, Tetsuo,Yamamoto, Tomofumi,Sato, Kunihiko,Jimbo, Masakazu,Imaoka, Tetsuo,Umeki, Yoshito Korean Nuclear Society 2014 Nuclear Engineering and Technology Vol.46 No.5
A feasibility study on the seismic design of nuclear reactor buildings with application of a seismic isolation system is introduced. After the Hyogo-ken Nanbu earthquake in Japan of 1995, seismic isolation technologies have been widely employed for commercial buildings. Having become a mature technology, seismic isolation systems can be applied to NPP facilities in areas of high seismicity. Two reactor buildings are discussed, representing the PWR and BWR buildings in Japan, and the application of seismic isolation systems is discussed. The isolation system employing rubber bearings with a lead plug positioned (LRB) is examined. Through a series of seismic response analyses using the so-named standard design earthquake motions covering the design basis earthquake motions obtained for NPP sites in Japan, the responses of the seismic isolated reactor buildings are evaluated. It is revealed that for the building structures examined herein: (1) the responses of both isolated buildings and isolating LRBs fulfill the specified design criteria; (2) the responses obtained for the isolating LRBs first reach the ultimate condition when intensity of motion is 2.0 to 2.5 times as large as that of the design-basis; and (3) the responses of isolated reactor building fall below the range of the prescribed criteria.
TETSUO KUBO,TOMOFUMI YAMAMOTO,KUNIHIKO SATO,MASAKAZU JIMBO,TETSUO IMAOKA,YOSHITO UMEKI 한국원자력학회 2014 Nuclear Engineering and Technology Vol.46 No.5
A feasibility study on the seismic design of nuclear reactor buildings with application of a seismic isolation systemis introduced. After the Hyogo-ken Nanbu earthquake in Japan of 1995, seismic isolation technologies have been widelyemployed for commercial buildings. Having become a mature technology, seismic isolation systems can be applied toNPP facilities in areas of high seismicity. Two reactor buildings are discussed, representing the PWR and BWR buildingsin Japan, and the application of seismic isolation systems is discussed. The isolation system employing rubber bearingswith a lead plug positioned (LRB) is examined. Through a series of seismic response analyses using the so-named standarddesign earthquake motions covering the design basis earthquake motions obtained for NPP sites in Japan, the responsesof the seismic isolated reactor buildings are evaluated. It is revealed that for the building structures examined herein: (1)the responses of both isolated buildings and isolating LRBs fulfill the specified design criteria; (2) the responses obtainedfor the isolating LRBs first reach the ultimate condition when intensity of motion is 2.0 to 2.5 times as large as that of thedesign-basis; and (3) the responses of isolated reactor building fall below the range of the prescribed criteria.
Nobuyoshi Kosaka,Akiko Kogure,Tomofumi Yamamoto,Fumihiko Urabe,Wataru Usuba,Marta Prieto-Vila,Takahiro Ochiya 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Liquid biopsy is indispensable for the resolution of current medical issues, such as the cost of developing new drugs and predicting responses of patients to drugs. In this sense, not only the technology for liquid biopsy but also the target biomolecules for biomarkers need to be identified. Extracellular vesicles (EVs), which contain various proteins, including membrane-bound proteins, and RNAs, including mRNA and long/short noncoding RNAs, have emerged as ideal targets for liquid biopsy. These complex biomolecules are covered by a lipid bilayer, which can protect them from degradation. In this review, we review current topics regarding EVs as cancer biomarkers and introduce technologies used for these recently emerged biomolecules.
Chemical variation in molecular cloud cores in the Orion A cloud. II.
Tatematsu, Ken'ichi,Ohashi, Satoshi,Umemoto, Tomofumi,Lee, Jeong-Eun,Hirota, Tomoya,Yamamoto, Satoshi,Choi, Minho,Kandori, Ryo,Mizuno, Norikazu Astronomical Society of Japan 2014 Publications of the Astronomical Society of Japan Vol.66 No.1