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The use of OverStitchTM for the treatment of intestinal perforation, fistulas and leaks
Thiruvengadam Muniraj,Harry R. Aslanian 소화기인터벤션의학회 2017 Gastrointestinal Intervention Vol.6 No.3
Gastrointestinal perforations, leaks and fistulas may complicate endoscopic and surgical procedures. Surgical repair is associated with significant morbidity. Therapeutic endoscopic tools and techniques have included the application of tissue sealants, clip closure, and stent placement. Endoscopic suturing is a rapidly evolving minimally invasive technique. The OverStitchTM (Apollo Endosurgery, USA) is currently the only available endoscopic suturing system. Although technically more difficult than clip closure, endoscopic suturing allows closure of larger defects. In some settings, outcomes similar to surgical management with less morbidity may be achieved. This review describes the OverStitchTM endoscopic suturing system and the published literature regarding its use for perforations, leaks and fistulas.
Mass Propagation of Vitex negundo L., in vitro
Thiruvengadam, Muthu,Jayabalan, Narayanasamypillai The Korean Society of Plant Biotechnology 2000 Plant molecular biology and biotechnology research Vol.2 No.3
Shoot proliferation was obtained from shoot tips and nodal explants of Vitex negundo L. on MS medium supplemented with either BAP or KIN (0.1-2.0 mg/L) alone or in combination with NAA (0.1 mg/L). The concentrations of cytokinins combined with NAA produced multiple shoots from shoot tips and nodal explants. The highest mean percentage (84.3$\pm$8.0) of shoot multiplication's were observed on nodal explants in the presence of BAP (1.5 mg/L) and NAA (0.1 mg/L) followed by shoot tips (65.0$\pm$5.0). The regenerated shootlets were rooted on MS basal medium IAA, IBA, NAA (0.1-1.5 mg/L). The maximum number of roots (51.0$\pm$2.6) was achieved on the medium containing IBA (1.0 mg/L) followed by other auxins (NAA, IAA). The regenerated plants were successfully transferred to a mixture of vermiculate and soil. About 95% of the plantlets survived when transferred to the field.
The use of OverStitchTM for the treatment of intestinal perforation, fistulas and leaks
Thiruvengadam Muniraj,Harry R. Aslanian 소화기인터벤션의학회 2017 International journal of gastrointestinal interven Vol.6 No.3
Gastrointestinal perforations, leaks and fistulas may complicate endoscopic and surgical procedures. Surgical repair is associated with significant morbidity. Therapeutic endoscopic tools and techniques have included the application of tissue sealants, clip closure, and stent placement. Endoscopic suturing is a rapidly evolving minimally invasive technique. The OverStitchTM (Apollo Endosurgery, USA) is currently the only available endoscopic suturing system. Although technically more difficult than clip closure, endoscopic suturing allows closure of larger defects. In some settings, outcomes similar to surgical management with less morbidity may be achieved. This review describes the OverStitchTM endoscopic suturing system and the published literature regarding its use for perforations, leaks and fistulas.
Thiruvengadam, Muthu,Rekha, K.T.,Yang, Chang-Hsien,Jayabalan, Narayanasamypillai,Chung, Ill-Min The Korean Society of Plant Biotechnology 2010 Plant biotechnology reports Vol.4 No.4
An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g $1^{-1}$ sucrose, 2.2 g $1^{-1}$ Gelrite, and 7.7 lM naphthalene acetic acid (NAA) with 2.2 ${\mu}M$ thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 ${\mu}M$ TDZ, 2.2 ${\mu}M$ NAA, and 3.3 ${\mu}M$ silver nitrate ($AgNO_3$). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 ${\mu}M$ gibberellic acid ($GA_3$). The elongated shoots were rooted in MS medium supplemented with 4.0 ${\mu}M$ indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.
Rajakumar, Govindasamy,Thiruvengadam, Muthu,Mydhili, Govindarasu,Gomathi, Thandapani,Chung, Ill-Min Springer-Verlag 2018 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.41 No.1
<P>Bio-mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out by utilizing the reducing and capping potential of Andrographis paniculata leaf extract. The capped ZnO NPs were characterized using UV-Vis, XRD, FTIR, SEM, TEM and SAED analyses. FTIR analysis suggested the role of phenolic compounds, terpenoids, and proteins of A. paniculata leaf extract, in nucleation and stability of ZnO NPs. XRD pattern compared with the standard confirmed spectrum of zinc oxide particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2 theta values. SEM and TEM analysis of ZnO NPs reveals those spherical and hexagonal shapes and the sizes at the range of 96-115 and 57 +/- 0.3 nm, respectively. The synthesized nanoparticles possess strong biological activities regarding anti-oxidant, anti-diabetic, and anti-inflammatory potentials which could be utilized in various biological applications by the cosmetic, food and biomedical industries.</P>
Hydrodynamics of co-current downward liquid-liquid system with packing
Swaminathan Samdavid,Thiruvengadam Renganathan,Kamatam Krishnaiah 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.1
The effect of packing and its voidage on the hydrodynamic characteristics of co-current downward liquidliquid system (CCDLLS) was studied using water and three different oils (exxsol, kerosene and white oil). Two flow regimes, homogeneously dispersed oil regime and heterogeneously dispersed oil, were observed with the total pressure drop showing a minimum at the regime transition velocity. Depending on the packing, frictional pressure drop either shows a minimum or monotonically increases with water velocity. Low-density oil results in higher holdup and correspondingly higher frictional pressure drop. Higher dispersed phase holdup is obtained by using a low voidage packing. Semi-empirical/empirical equations are proposed to predict the frictional pressure drop and phase holdup.