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      • Use of infinite elements in simulating liquefaction phenomenon using coupled approach

        Kumari, Sunita,Sawant, V.A. Techno-Press 2013 Coupled systems mechanics Vol.2 No.4

        Soils consist of an assemblage of particles with different sizes and shapes which form a skeleton whose voids are filled with water and air. Hence, soil behaviour must be analyzed by incorporating the effects of the transient flow of the pore-fluid through the voids, and therefore requires a two-phase continuum formulation for saturated porous media. The present paper presents briefly the Biot's basic theory of dynamics of saturated porous media with u-P formulation to determine the responses of pore fluid and soil skeleton during cyclic loading. Kelvin elements are attached to transmitting boundary. The Pastor-Zienkiewicz-Chan model has been used to describe the inelastic behavior of soils under isotropic cyclic loadings. Newmark-Beta method is employed to discretize the time domain. The response of fluid-saturated porous media which are subjected to time dependent loads has been simulated numerically to predict the liquefaction potential of a semi-infinite saturated sandy layer using finite-infinite elements. A settlement of 17.1 cm is observed at top surface. It is also noticed that liquefaction occurs at shallow depth. The mathematical advantage of the coupled finite element analysis is that the excess pore pressure and displacement can be evaluated simultaneously without using any empirical relationship.

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        Deciphering genome-wide WRKY gene family of Triticum aestivum L. and their functional role in response to Abiotic stress

        Saurabh Gupta,Vinod Kumar Mishra,Sunita Kumari,Raavi,Ramesh Chand,Pritish Kumar Varadwaj 한국유전학회 2019 Genes & Genomics Vol.41 No.1

        WRKY transcription factors (TFs) act in regulating plant growth and development as well as in response to different stress. Some earlier studies done by individual researchers reported different wheat WRKY TFs. Although, the recently released wheat genome has opened an avenue to investigate wheat WRKYs (TaWRKY) TFs. Prime objective of this study to performed genome-wide classifications of TaWRKYs and their functional annotation. The classification of 107 individual identified characterized sequences of TaWRKY (IICS-TaWRKY) and 160 uncharacterized draft sequences of TaWRKY (UDS-TaWRKY), along with their gene structures and motifs analysis was performed. Along with comparative sequence analysis and microarray analysis was performed to mimic out TaWRKYs functions in response to different abiotic stresses, accompanied by in-vitro validation. The comparative phylogenetic analysis and estimation of Ka/Ks ratio with Triticum urartu, illustrate group based clasifications of TaWRKYs and evolutionary divergences. Furthermore, motif-based and protein-DNA interaction analysis of TaWRKYs helps to identify, their putative function in target DNA recognition sites. Subsequently, results of microarray and comparative sequence analysis provides the evidence of TaWRKYs involved in heat and/or drought stress. Further, in-vitro results validates that TaWRKY014, TaWRKY090 are found to participate in response of drought stress, whereas TaWRKY008, TaWRKY122, and WRKY45 are involved in response of heat and drought stress. These findings can be utilized in developing novel heat and drought-tolerant wheat cultivars using marker-assisted breeding and transgenic development.

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