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The SAR92 Clade: an Abundant Coastal Clade of Culturable Marine Bacteria Possessing Proteorhodopsin
Stingl, Ulrich,Desiderio, Russell A.,Cho, Jang-Cheon,Vergin, Kevin L.,Giovannoni, Stephen J. American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.7
<B>ABSTRACT</B><P>Proteorhodopsin (PR) is a protein that is abundant in marine bacterioplankton. PR is hypothesized to be a light-dependent proton pump, thus creating a proton gradient that can be used for energy production without electron transport. Currently, the only culture that has been reported to possesses PR is the highly abundant alphaproteobacterium “<I>Candidatus</I> Pelagibacter ubique” (SAR11 clade), but surprisingly, its growth in batch culture was not enhanced by light. Here, we present the first cultured gammaproteobacterium that possesses a PR gene. Genome sequencing and analysis of HTCC2207 showed that the PR gene is present as a lone transcriptional unit directly followed by an operon containing genes that are presumably involved in the synthesis of retinal, the chromophore of PR. Half-time decay times of different PR intermediates in native HTCC2207 cells ranged between 2 and 15 ms, and the absorbance maximum of PR was determined to be 528 nm. Proteorhodopsin was identified in three additional strains, using a specific PCR assay on other cultured members of the SAR92 clade. Phylogenetic analyses of the PR genes determined that they form a deeply rooting cluster not closely related to any PR genes recovered so far. Fluorescence in situ hybridization and RNA blots showed that the SAR92 clade reaches up to 10% of the total bacterial population in surface waters close to the Oregon coast and decreases over depth and distance from the shore. Although the growth of HTCC2207 is limited by the amount of available carbon that is present in the medium applied, these cultures do not grow at higher rates nor do they have higher growth yields when incubated under light.</P>
Genome Sequence of the Novel Marine Member of the Gammaproteobacteria Strain HTCC5015
Thrash, J. Cameron,Stingl, Ulrich,Cho, Jang-Cheon,Ferriera, Steve,Johnson, Justin,Vergin, Kevin L.,Giovannoni, Stephen J. American Society for Microbiology 2010 Journal of Bacteriology Vol.192 No.14
<B>ABSTRACT</B><P>HTCC5015 is a novel, highly divergent marine member of the <I>Gammaproteobacteria</I>, currently without a cultured representative with greater than 89% 16S rRNA gene identity to itself. The organism was isolated from water collected from Hydrostation S south of Bermuda using high-throughput dilution-to-extinction culturing techniques. Here we present the genome sequence of the unique <I>Gammaproteobacterium</I> strain HTCC5015.</P>