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( Siddikee Md. Ashaduzzaman ),( Sherlyn C. Tipayno ),( Ki Yoon Kim ),( Jong Bae Chung ),( Tong Min Sa ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.4
To study the effects of salinity-sodicity on bacterial population and enzyme activities, soil samples were collected from the Bay of Yellow Sea, Incheon, South Korea. In the soils nearest to the coastline, pH, electrical conductivity (ECe), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP) were greater than the criteria of salinesodic soil, and soils collected from sites 1.5-2 km away from the coastline were not substantially affected by the intrusion and spray of seawater. Halotolerant bacteria showed similar trends, whereas non-tolerant bacteria and enzymatic activities had opposite trends. Significant positive correlations were found between EC, exchangeable Na+, and pH with SAR and ESP. In contrast, ECe, SAR, ESP, and exchangeable Na+ exhibited significant negative correlations with bacterial populations and enzyme activities. The results of this study indicate that the soil chemical variables related with salinity-sodicity are significantly related with the sampling distance from the coastline and are the key stress factors, which greatly affect microbial and biochemical properties.
( Siddikee M. A. ),( P. S. Chauhan ),( R. Anandham ),( Gwang Hyun Han ),( Tong Min Sa ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
In total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the Yellow Sea, near the city of Incheon in the Republic of Korea. All of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (IAA), nitrogen fixation, phosphorus (P) and zinc (Zn) solubilization, thiosulfate (S2O3) oxidation, the production of ammonia (NH3), and the production of extracellular hydrolytic enzymes such as protease, chitinase, pectinase, cellulase, and lipase under in vitro conditions. From the original 140 strains tested, on the basis of the latter tests for plant growth promotional activity, 36 were selected for further examination. These 36 halotolerant bacterial strains were then tested for 1- aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Twenty-five of these were found to be positive, and to be exhibiting significantly varying levels of activity. 16S rRNA gene sequencing analyses of the 36 halotolerant strains showed that they belong to 10 different bacterial genera: Bacillus, Brevibacterium, Planococcus, Zhihengliuella, Halomonas, Exiguobacterium, Oceanimonas, Corynebacterium, Arthrobacter, and Micrococcus. Inoculation of the 14 halotolerant bacterial strains to ameliorate salt stress (150 mM NaCl) in canola plants produced an increase in root length of between 5.2% and 47.8%, and dry weight of between 16.2% and 43%, in comparison with the uninoculated positive controls. In particular, three of the bacteria, Brevibacterium epidermidis RS15, Micrococcus yunnanensis RS222, and Bacillus aryabhattai RS341, all showed more than 40% increase in root elongation and dry weight when compared with uninoculated saltstressed canola seedlings. These results indicate that certain halotolerant bacteria, isolated from coastal soils, have a real potential to enhance plant growth under saline stress, through the reduction of ethylene production via ACC deaminase activity.
Siddikee, Md. Ashaduzzaman,Sundaram, Subbiah,Chandrasekaran, Murugesan,Kim, Kiyoon,Selvakumar, Gopal,Sa, Tongmin The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.
Optimization of gibberellic acid production by Methylobacterium oryzae CBMB20
Ashaduzzaman Siddikee,Muhammad Hamayun,Gwang-Hyun Han(한광현),Tong-min Sa(사동민) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.4
지베렐린산은 곰팡이 및 세균으로부터 생산되는 주요 식물생장촉진물질이며 다양한 산업에서 이용되고 있다. 본 연구는 Methylobacterium oryzae CBMB20을 이용하여 GA₃를 생산하기 위해 탄소원 및 질소원을 선발하였으며, 선발된 탄소원 및 질소원의 최적 농도와 최적 비율을 조사하였다. 탄소원으로는 Na-succinate가 methanol, glucose, maltose, sucrose, fructose, lactose에 비해 가장 우수하였으며, 질소원으로는 NH4Cl가 NO₃, NaNO₃, glycine 등에 비해 우수하였다. 배양액에서 Nasuccinate와 NH₄Cl를 각각 5 및 0.4 g L<SUP>-1</SUP> 농도 비율로 사용하였을 때 GA₃의 생산량이 가장 높았다. 또한 ammonium mineral salt 배지의 pH를 7로 유지하고 30℃의 조건으로 96시간 배양하였을 때 GA₃의 생산이 최대로 나타나는 것을 확인하였다. Gibberellic acid (GA₃) is used in many industries and constitutes the primary gibberellins produced by fungi and bacteria. However, there is no information on GA₃ production by Methylobacterium oryzae CBMB20, a novel plant growth promoting bacterium. We investigated the favorable carbon (C) and nitrogen (N) sources and ratios and cultural conditions, such as incubation temperature, pH of the culture medium, and incubation period for the maximum production of GA₃ by Methylobacterium oryzae CBMB20. Maximum GA₃ production was observed in ammonium mineral salt (AMS) broth supplemented with Na-succinate and NH₄Cl as C and N sources, respectively. The maximum GA₃ production was found at the C/N ratio of 5:0.4 g L<SUP>-1</SUP>. The highest GA₃ production was obtained when the bacterial culture was incubated at 30oC for 96 h at pH 7.
Md. Ashaduzzaman Siddikee,Subbiah Sundaram,MURUGESANCHANDRASEKARAN,김기윤,셀바,사동민 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane- 1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.