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- 저자 : Shanyan Yang (검색결과 3 건)
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Optimization of Pine Pollen Fermentation Conditions Using Lactobacillus paracasei
Shanyan Yang,Hailong Li 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
To improve the nutritional value of pine pollen, a statistical experimental design strategy was applied to optimize the fermentation conditions of pine pollen using Lactobacillus paracasei Lc-3. Influence of the inoculum size, temperature, and initial pH on viable counts and crude protein production was evaluated using a Plaackett-Burman design using the steepest ascent method to approach the experimental design space. Factors were optimized using a Box-Behnken design and response surface methodology. A quadratic model was found to fit the viable counts and crude protein amounts. Optimum values of the tested variables for production of viable counts and crude protein were an inoculum size of 11.92%, temperature=39.60oC, and an initial pH of 7.22. Under optimized conditions, the viable count was 4.24×109 CFU/mL and the crude protein amount was 15.35% (dry matter). A single factor experiment as preliminary research for fermentation product treatment was performed.
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A Bacillus coagulans and its Application in Pine Pollen Fermentation
Shanyan Yang,Yaoyao Wu,Chubo Luo,Zhihong Di,Yunpeng Wu,Jie Zhang,Yaomei Xu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6
In the study, a potential L-lactic acid-producing bacillus-strain LT1 was isolated. It was identified as Bacillus coagulans based on its morphological, physiological, biochemical properties, and phylogenetic similarity of 16S rDNA. The fermentation conditions of pine pollen by strain LT1 were optimized using Box-Behnken design and response surface methodology. Optimum values of the tested variables for production of viable counts were an inoculum size of 9.22%, temperature= 49.21℃, and an initial pH of 6.82. Under optimized conditions, the viable count was 5.49×109 CFU/mL. Finally, the single factor experiment as preliminary research for fermentation product treatment was performed.
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Acetylation Enhances the Promoting Role of AIB1 in Breast Cancer Cell Proliferation
Shan Yan,Dingyun You,Hongbo Zhao,Yan Wang,Yang Jiao,Minnan Lu 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.9
The oncogene nuclear receptor coactivator amplified in breast cancer 1 (AIB1) is a transcriptional coactivator, which is overexpressed in various types of human cancers, including breast cancer. However, the molecular mechanisms regulating AIB1 function remain largely unknown. In this study, we present evidence demonstrating that AIB1 is acetylated by MOF in human breast cancer cells. Moreover, we also found that the acetylation of AIB1 enhances its function in promoting breast cancer cell proliferation. We further showed that the acetylation of AIB1 is required for its recruitment to E2F1 target genes by E2F1. More importantly, we found that the acetylation levels of AIB1 are greatly elevated in human breast cancer cells compared with that in non-cancerous cells. Collectively, our results shed light on the molecular mechanisms that regulate AIB1 function in breast cancer.
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