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      저자 : Seyedeh Sara Shafiei (검색결과 1 건)
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        Amine-functionalized Single-walled Carbon Nanotube/Polycaprolactone Electrospun Scaffold for Bone Tissue Engineering: in vitro Study

        Hadi Tohidlou,Seyedeh Sara Shafiei,Shahsanam Abbasi,Mitra Asadi-Eydivand,Mehrnoosh Fathi-Roudsari 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

        Carbon nanotubes (CNT) are beneficent candidates for bone tissue engineering (BTE) applications, mostlybecause of their superior mechanical properties. Although the previous studies confirmed that single-walled carbonnanotubes (SWNTs) have significant effect on biomedical applications but there is no study reported the effect of SWNTs onproperties of the PCL scaffolds for BTE applications. The purpose of this study was to evaluate the effect of aminefunctionalizedsingle-walled carbon nanotubes (aSWNTs) on mechanical properties and in vitro behavior of Polycaprolacton(PCL) scaffolds. PCL as a biocompatible polymeric matrix was composited with different amounts (ranging from 0, 0.1, 0.2,0.5 wt.%) of aSWNTs to enhance structural and functional properties of electrospun scaffolds. Attachment, proliferation,differentiation of rat bone marrow-derived mesenchymal stem cells (rMSCs) seeded onto the scaffolds was analyzed. Themorphology and mechanical properties of the scaffolds were characterized using SEM and tensile test. The results indicatedthat the addition of aSWNTs heightened the tensile strength while bioactivity and degradation rate were increased. Also, theaddition of aSWNTs has significantly amplified the electrical conductivity of PCL solution and resulted in the thinner fiberswith more uniform size distribution. Attachment, proliferation and differentiation of rMSCs were significantly improved. Although the best mechanical property was achieved in the scaffold with 0.2 wt% aSWNT, but the composite scaffold with0.5 wt% aSWNT significantly shows superior proliferation and differentiation of the rMSCs. Alkaline phosphatase activitydemonstrated elevated differentiation of cells on nanocomposite scaffolds.

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