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- 저자 : Sepideh Ghorbani (검색결과 1 건)
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Sepideh Ghorbani,Fatemeh Tabandeh,Bagher Yakhchali,Mohammad Reza Mehrnia 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5
A cell surface display system with metalbinding properties was previously developed using CS3fimbriae, which are hollow tubes 20 nm-thick and 2 nm in diameter. In this study, hybrid CS3 pili were separated from recombinant Escherichia coli and entrapped in calcium alginate gel beads in order to improve their stabilization and also adsorption of heavy metals. The surface morphology of the gel beads containing pili was investigated by scanning electron microscopy (SEM). Immunofluorescence microscopy was employed to confirm the attachment of nanobiofibers to the alginate beads. The effects of three variables (sodium alginate concentration,protein to alginate mass ratio, and bead size) at two levels each on Cd^(2+) biosorption efficiency were investigated by full factorial experimental design. A second-order polynomial equation modeled the design space for the process response of cadmium removal capacity. The optimal values of the factors were obtained as follows: 1% sodium alginate concentration, 0.25 protein to alginate mass ratio,and a 6 mm bead size. Under these conditions, Cd^(2+) was adsorbed at 45.45 mg/g to the nanobiofiber. The results indicate that the immobilized recombinant hybrid CS3 pili may be an appropriate biosorbent for removal of heavy metals from polluted aquatic environments.
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