A study on the Improvement of Shandong Logistics Park

Fengfei Sun,Ruonan Zhang,Fan Zhang 한국유통과학회 2015 한국유통과학회 학술대회 논문집 Vol.2015 No.-

This study is to investigate how to promote value added logistics activities for Shandong Province of China. the logistics industry will play more and more important role as assistance service in national economy development. Logistics Park is the new format of modern logistics industry, which develops very rapidly by integrating logistics functions to meet the logistics demands of the port, industrial parks and urban development. It may become one of the main forms of the integration of the logistics industry in future. Therefore, the planning and construction of modern logistics park is great significant, promoting China's industry and the rapid development of the logistics park play intensive, transport convergence and the support of the joint expansion, and enhance logistics services and management levels, and promote regional economic development.

The Necessity of China’s SMEs Developing Overseas Market

Zhang ruonan,Sun fengfei 한중경제문화학회 2021 한중경제문화연구 Vol.16 No.-

Home Government Policies and the Internationalization of SMEs

Zhang Ruonan,Sun Fengfei 한중경제문화학회 2021 한중경제문화연구 Vol.17 No.-

Screening and functional validation of lipid metabolism-related lncRNA-46546 based on the transcriptome analysis of early embryonic muscle tissue in chicken

Chen Ruonan,Liao Kai,Liao Herong,Zhang Li,Zhao Haixuan,Sun Jie 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.2

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolismrelated genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

Serum exosomal miR-192 serves as a potential detective biomarker for early pregnancy screening in sows

Gao Ruonan,Li Qingchun,Qiu Meiyu,Xie Su,Sun Xiaomei,Huang Tao 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.9

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC] = 0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

Influence of Bisphenol A on the transport and deposition behaviors of bacteria in quartz sand

Wu, Dan,He, Lei,Sun, Ruonan,Tong, Meiping,Kim, Hyunjung Elsevier 2017 Water research Vol.121 No.-

<P><B>Abstract</B></P> <P>The influence of Bisphenol A (BPA) on the transport and deposition behaviors of bacteria in quartz sand was examined in both NaCl (10 and 25 mM) and CaCl<SUB>2</SUB> solutions (1.2 and 5 mM) by comparing the breakthrough curves and retained profiles of cell with BPA in suspensions versus those without BPA. Gram-negative <I>Escherichia coli</I> and Gram-positive <I>Bacillus subtilis</I> were employed as model cells in the present study. The extended Derjaguin-Landau-Verwey-Overbeek interaction energy calculation revealed that the presence of BPA in cell suspensions led to a lower repulsive interaction between the cells and the quartz sand. This suggests that, theoretically, increased cell deposition on quartz sand would be expected in the presence of BPA. However, under all examined solution conditions, the presence of BPA in cell suspensions increased transport and decreased deposition of bacteria in porous media regardless of cell type, ionic strength, ion valence, the presence or absence of extracellular polymeric substances. We found that competition by BPA through hydrophobicity for deposition sites on the quartz sand surfaces was the sole contributor to the enhanced transport and decreased deposition of bacteria in the presence of BPA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transport of bacteria in porous media was affected by Bisphenol A (BPA). </LI> <LI> BPA increased the transport of both Gram-negative and Gram-positive cells. </LI> <LI> BPA increased cell transport regardless of ionic strength, ion valence, and EPS. </LI> <LI> Repulsive interaction between bacteria and quartz sand was decreased by BPA. </LI> <LI> Competition deposition sites by BPA drove to the increased cell transport. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Lingling Zhou,Yuxi Di,Mingfei Zhang,Yichang Chen,Ruonan Sun,Meiyu Shen,Fengxiang Tian,Pei Yang,Feiya Qian 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.1

Purpose: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component in Rehmanniae Radix (RR), has been found to regulate human immunity. However, the effects of CAT on Th17 cell differentiation and improvement of RA are not clear. Materials and Methods: Collagen-induced arthritis (CIA) mice were constructed to detect the effects of CAT on arthritis and Th17 cells. The effect of CAT on Th17 differentiation was evaluated with let-7g-5p transfection experiments. Flow cytometry was used to detect the proportion of Th17 cells after CAT treatment. Levels of interleukin-17 and RORγt were assessed by qRT-PCR and enzyme-linked immunosorbent assay. The expression of signal transducer and activator of transcription 3 (STAT3) was determined by qRT-PCR and Western blot. Results: We found that the proportion of Th17 cells was negatively associated with let-7g-5p expression in CIA mice. In in vitro experiments, CAT suppressed traditional differentiation of Th17 cells. Simultaneously, CAT significantly decreased Tregs-to-Th17 cells transdifferentiation. Our results demonstrated that CAT inhibited Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p and that the suppressive effect of CAT on traditional differentiation of Th17 cells is not related with let-7-5p. Conclusion: Our data indicate that CAT may be a potential modulator of Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p to reduce the expression of STAT3. These results provide new directions for research into RA treatment.