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        Photosynthetic response of Arctic kelp zoospores exposed to radiation and thermal stress

        Roleda, Michael Y. Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.9

        Zoospores of Arctic kelp species, Alaria esculenta, Laminaria digitata and Saccharina latissima were exposed to different temperature ($2^{\circ}C$ to $19^{\circ}C$) and radiation (photosynthetically active radiation (PAR = P), PAR + UV-A (PA), and PAR + UV-A + UV-B (PAB)) conditions in the laboratory. Species-specific responses to the combined effect of light and temperature stress showed sensitivity in the order S. latissima > L. digitata > A. esculenta. The optimum temperature range for photosynthesis in different Arctic kelp species' zoospores was between $7-13^{\circ}C$, temperatures higher than in the natural environment. Short-term response to increasing temperature was non-lethal while moderate temperature increase had an ameliorating effect on the overall biological effect of UVR; where the lowest photoinhibition was observed at $13^{\circ}C$ under PAB and higher photosynthetic recovery was observed in UVR-pre-exposed zoospores at $7-13^{\circ}C$ compared to $2^{\circ}C$. Above the temperature optima, continued cultivation under high temperature had a negative impact on the recovery of photoinhibition. The higher capacity for non-photochemical quenching (NPQ) in A. esculenta and L. digitata helped to regulate and protect photosynthesis under light and temperature stress compared to S. latissima. The investigated Arctic kelp species may be able to locally survive under the influence of UVR at a certain range of temperature increase but the southernmost distribution range of the species may shift to higher latitudes; although natural selection may result in genotypes adapted to stressful environment.

      • SCIESCOPUSKCI등재

        Discovery of novel haplotypes from wild populations of Kappaphycus (Gigartinales, Rhodophyta) in the Philippines

        Roleda, Michael Y.,Aguinaldo, Zae-Zae A.,Crisostomo, Bea A.,Hinaloc, Lourie Ann R.,Projimo, Vicenta Z.,Dumilag, Richard V.,Lluisma, Arturo O. The Korean Society of Phycology 2021 ALGAE Vol.36 No.1

        As the global demand for the carrageenophyte Kappaphycus is steadily increasing, its overall productivity, carrageenan quality, and disease resistance are gradually declining. In the face of this dilemma, wild Kappaphycus populations are viewed as sources of new cultivars that could potentially enhance production; therefore, assessment of their diversity is crucial. This study highlights the morphological and genetic diversity of wild Kappaphycus species obtained from two sites in the Philippines. Nucleotide alignments of available 5' region of the mitochondrial cytochrome c oxidase subunit I (COI-5P) and cox2-3 spacer sequences of Kappaphycus confirmed the presence of K. alvarezii in Guiuan, Eastern Samar and K. striatus in Bolinao, Pangasinan. Based on the concatenated sequences of the COI-5P and the cox2-3 spacer, nine novel haplotypes were observed along with other published haplotypes. However, there was no relationship between haplotype and morphology. These newly recognized haplotypes indicate a reservoir of unutilized wild genotypes in the Philippines, which could be taken advantage of in developing new cultivars with superior traits. DNA barcodes generated from this study effectively expand the existing databank of Kappaphycus sequences and can provide insights in elucidating the genetic diversity of Kappaphycus species in the country.

      • SCIESCOPUSKCI등재

        Occurrence and pathogenicity of Pythium (Oomycota) on Ulva species (Chlorophyta) at different salinities

        Herrero, Maria-Luz,Brurberg, May Bente,Ojeda, Dario I.,Roleda, Michael Y. The Korean Society of Phycology 2020 ALGAE Vol.35 No.1

        Pythium species are ubiquitous organisms known to be pathogens to terrestrial plants and marine algae. While several Pythium species (hereafter, Pythium) are described as pathogens to marine red algae, little is known about the pathogenicity of Pythium on marine green algae. A strain of a Pythium was isolated from a taxonomically unresolved filamentous Ulva collected in an intertidal area of Oslo fjord. Its pathogenicity to a euryhaline Ulva intestinalis collected in the same area was subsequently tested under salinities of 0, 15, and 30 parts per thousand (ppt). The Pythium isolate readily infected U. intestinalis and decimated the filaments at 0 ppt. Mycelium survived on U. intestinalis filaments for at least 2 weeks at 15 and 30 ppt, but the infection did not progress. Sporulation was not observed in the infected algal filaments at any salinity. Conversely, Pythium sporulated on infected grass pieces at 0, 15, and 30 ppt. High salinity retarded sporulation, but did not prevent it. Our Pythium isolate produced filamentous non-inflated sporangia. The sexual stage was never observed and phylogenetic analysis using internal transcribed spacer suggest this isolate belongs to the clade B2. We conclude that the Pythium found in the Oslo fjord was a pathogen of U. intestinalis under low salinity.

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