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Kumar, Arthikala Manoj,Sreevathsa, Rohini,Reddy, Kalpana Nanja,Ganesh, Prasa Trichy,Udayakumar, Makarla 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the $T_1$ generation seedlings on 300 mg $L^{-1}$ kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the $T_2$ generation plants proved the stability of the transgene indicating the feasibility of the method.
Arthikala Manoj Kumar,Rohini Sreevathsa,Kalpana Nanja Reddy,Prasa Trichy Ganesh,Makarla Udayakumar 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the T1 generation seedlings on 300 mg L^(-1) kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the T2 generation plants proved the stability of the transgene indicating the feasibility of the method.
Mahesh Patil,T. G. Prasad,S. V. Ramu,P. Jathish,Rohini Sreevathsa,P. Chandrashekar Reddy,M. Udayakumar 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2
Groundnut (Arachis hypogaea L.) is animportant oilseed crop grown in semi-arid tropics where itexperiences moisture stress at different stages of growthresulting in reduced growth and productivity. In this study,we report that the stress tolerance of groundnut can beimproved by overexpression of stress-specific transcriptionfactor through transgenic approach. In silico electronicnorthernanalysis of AtNAC2 showed increased expressionunder different abiotic stresses. The transcript levels of ahomolog of AtNAC2 gene were upregulated under differentdrought regimes in groundnut. Groundnut transgenicsoverexpressing AtNAC2 showed enhanced tolerance todrought and salinity with improved yield under waterlimitedconditions. The study demonstrates that AtNAC2 isa potential candidate gene to improve stress tolerance bytransgenic approach.