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RISS 인기검색어
- 검색키워드
- 저자 : Paunesku, Tatjana (검색결과 2 건)
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AIGUO WU,TATJANA PAUNESKU,ERIC M. B. BROWN,ANGELA BABBO,CECILLE CRUZ,MOHAMMED ASLAM,VINAYAK DRAVID,GAYLE E. WOLOSCHAK 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.1
This work demonstrates the assembly of TiO2 nanoparticles with attached DNA oligonucleotides into a 3D mesh structure by allowing base pairing between oligonucleotides. A change of the ratio of DNA oligonucleotide molecules and TiO2 nanoparticles regulates the size of the mesh as characterized by UV-visible light spectra, transmission electron microscopy (TEM) and atomic force microscopy (AFM) images. This type of 3D mesh, based on TiO2-DNA oligonucleotide nanoconjugates, can be used for studies of nanoparticle assemblies in materials science, energy science related to dye-sensitized solar cells, environmental science as well as characterization of DNA interacting proteins in the field of molecular biology. As an example of one such assembly, proliferating cell nuclear antigen protein (PCNA) was cloned, its activity was verified, and the protein was purified, loaded onto double strand DNA oligonucleotide-TiO2 nanoconjugates, and imaged by atomic force microscopy. This type of approach may be used to sample and perhaps quantify and/or extract specific cellular proteins from complex cellular protein mixtures based on their affinity for chosen DNA segments assembled into the 3D matrix.
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Falaschetti, Christine A.,Paunesku, Tatjana,Kurepa, Jasmina,Nanavati, Dhaval,Chou, Stanley S.,De, Mrinmoy,Song, MinHa,Jang, Jung-tak,Wu, Aiguo,Dravid, Vinayak P.,Cheon, Jinwoo,Smalle, Jan,Woloschak, G American Chemical Society 2013 ACS NANO Vol.7 No.9
<P>The multicatalytic ubiquitin–proteasome system (UPS) carries out proteolysis in a highly orchestrated way and regulates a large number of cellular processes. Deregulation of the UPS in many disorders has been documented. In some cases, such as carcinogenesis, elevated proteasome activity has been implicated in disease development, while the etiology of other diseases, such as neurodegeneration, includes decreased UPS activity. Therefore, agents that alter proteasome activity could suppress as well as enhance a multitude of diseases. Metal oxide nanoparticles, often developed as diagnostic tools, have not previously been tested as modulators of proteasome activity. Here, several types of metal oxide nanoparticles were found to adsorb to the proteasome and show variable preferential binding for particular proteasome subunits with several peptide binding “hotspots” possible. These interactions depend on the size, charge, and concentration of the nanoparticles and affect proteasome activity in a time-dependent manner. Should metal oxide nanoparticles increase proteasome activity in cells, as they do <I>in vitro</I>, unintended effects related to changes in proteasome function can be expected.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-9/nn402416h/production/images/medium/nn-2013-02416h_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn402416h'>ACS Electronic Supporting Info</A></P>
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