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RISS 인기검색어
- 검색키워드
- 저자 : Novi Angeline (검색결과 2 건)
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Precise Electrical Detection of Curcumin Cytotoxicity in Human Liver Cancer Cells
Novi Angeline,추성식,김철휘,방석호,김태형 한국바이오칩학회 2021 BioChip Journal Vol.15 No.1
Curcumin is a polyphenol extracted from the roots of Curcuma plants that exerts potential anticancer effects. However, owing to its low toxicity, curcumin is known to be effective only at high doses. Here, we report the highly sensitive assessment of curcumin toxicity in human liver cancer cells, which was facilitated using a fabricated conductive platform and an electrochemical detection method. To identify the best platform for assessing cell viability, both electrochemical deposition time (0–150 s) and RGD peptide concentrations (0–0.1 mg/mL) were varied. Unlike other cell types, liver cancer cells showed the highest electrical signals without the modification of cell adhesion peptides. With 120 s of gold deposition under peptide-free conditions, 10,000 cells/chip were detectable within a linear range of 10,000–500,000 cells. Notably, even at low concentrations (e.g., 20 μM), curcumin could inhibit liver cancer cell-specific electrical signals by 45%, which would be undetectable in conventional colorimetric assay (CCK-8). The half-maximal inhibitory concentration (IC50) of curcumin for HepG2 cells was 23.63 μM, which indicates 3.71-fold higher sensitivity than that determined in the CCK-8 assay (IC50 = 87.88 μM). Hence, the developed conductive cell culture platform, in combination with the electrochemical detection method, could serve as a promising tool for detecting toxicity and/or anticancer activity of food-derived compounds in cancer/normal cells.
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Nanomaterial-modified Hybrid Platforms for Precise Electrochemical Detection of Dopamine
인탄 로살리나,Novi Angeline,김태형 한국바이오칩학회 2019 BioChip Journal Vol.13 No.1
Dopamine belongs to the class of catecholamine neurotransmitters which have vital roles in the human central nervous system. Due to its importance in signal transmission in the nervous system, the abnormal release of dopamine is critical for the development of a number of neurological diseases/disorders, including Parkinson’s diseases, attention deficit hyperactivity disorder, and even drug addiction. Hence, there is an utmost need to develop platforms for the quantitative detection of dopamine in the human body in a rapid, sensitive, and label-free manner. A variety of nanomaterials have been explored and integrated into dopamine sensing platforms; in particular, electrochemical sensors can enhance both the sensitivity and selectivity toward dopamine, with promising results. The aim of this review is to summarize recent research on nanomaterial-modified dopamine electrochemical sensor platforms, particularly those that use nanoparticle-, graphene composite-, and transition metal dichalcogenide-modified electrodes. The information presented in this review might motivate the discovery or extension of nanomaterials with beneficial properties for the development of biosensors to detect various neurotransmitters including dopamine.
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