http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Complete Genome Sequence of Pseudarthrobacter sp. IC2-21, a Fluquinconazole-Degrading Soil Bacterium
Riu Myoungjoo,Kim Songhwa,Song Jaekyeong 한국미생물·생명공학회 2024 한국미생물·생명공학회지 Vol.52 No.1
Pseudarthrobacter sp. IC2-21 is isolated from the greenhouse soil in Icheon, Gyeonggi, Korea. This strain IC2-21 is a first fluquinconazole degrading soil bacterium. We analyze the whole genome sequence of Pseudarthrobacter sp. IC2-21. The sequence analysis revealed that Pseudarthrobacter sp. IC2-21 possesses a single 4,265,009 bps circular chromosome with a DNA G+C-content of 65.4%. This chromosome contains 3,942 protein-coding sequences and 12 rRNA and 51 tRNA genes. In the result of sequence analysis, it is revealed that strain IC2-21 possessed genes coding the triazole pesticides degradation related enzymes, such as oxygenase, and fluquinconazole degradation related genes.
Myoungjoo Riu,Man Su Kim,최수근,오상근,CHOONG-MIN RYU 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.7
Bacterial volatile compounds (BVCs) exert beneficial effects on plant protection both directly and indirectly. Although BVCs have been detected in vitro, their detection in situ remains challenging. The purpose of this study was to investigate the possibility of BVCs detection under in situ condition and estimate the potentials of in situ BVC to plants at below detection limit. We developed a method for detecting BVCs released by the soil bacteria Bacillus velezensis strain GB03 and Streptomyces griseus strain S4-7 in situ using solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS). Additionally, we evaluated the BVC detection limit in the rhizosphere and induction of systemic immune response in tomato plants grown in the greenhouse. Two signature BVCs, 2-nonanone and caryolan-1-ol, of GB03 and S4-7 respectively were successfully detected using the soil-vial system. However, these BVCs could not be detected in the rhizosphere pretreated with strains GB03 and S4-7. The detection limit of 2-nonanone in the tomato rhizosphere was 1 µM. Unexpectedly, drench application of 2-nonanone at 10 nM concentration, which is below its detection limit, protected tomato seedlings against Pseudomonas syringae pv. tomato. Our finding highlights that BVCs, including 2-nonanone, released by a soil bacterium are functional even when present at a concentration below the detection limit of SPME-GC-MS.
Lee, Hyeri,Riu, Myoungjoo,Kim, Eunhye,Moon, Joon-Kwan,Choi, Hoon,Do, Jung-A,Oh, Jae-Ho,Kwon, Ki-Sung,Lee, Young Deuk,Kim, Jeong-Han 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2
A single residue analytical method was developed for herbicide chlorpropham in various crops. Brown rice, apple, mandarin, Kimchi cabbage, green pepper, potato, and soybean were selected as representative crops, and clean-up system, partition solvent, and extraction solvent were optimized. For high performance liquid chromatography (HPLC), $C_{18}$ column was used with elution solvents of water and acetonitrile. Limit of quantitation (LOQ) of chlorpropham was 2 ng (S/N >20), and excellent linearity ($R^2$=1.000) was achieved. Method limit of quantitation (MLOQ) was 0.02 mg/kg. For recovery tests, crop samples were macerated and fortified with chlorpropham standard solution at three fortification levels (MLOQ, 10 MLOQ, and 100 MLOQ). And then those were extracted with acetonitrile, concentrated and partitioned with n-hexane. The n-hexane layer was then concentrated, cleaned-up through Florisil$^{(R)}$ column with ethyl acetate:n-hexane (5:95, v/v) prior to concentration and analysis with HPLC. Good recoveries from 76.8 to 107.9% with coefficients of variation of less than 10% were obtained, regardless of sample type, which satisfies the criteria of Korea Food and Drug Administration. Those results were confirmed with liquid chromatography-mass spectrometry (LC-MS). The method established in this study could be applied to most of crops as an official and general method for the analysis of chlorpropham residue.
이혜란,정지혜,류명주,류충민,Lee, Hae-Ran,Jung, Jihye,Riu, Myoungjoo,Ryu, Choong-Min 한국식물병리학회 2017 식물병연구 Vol.23 No.2
전 세계적으로 주요 작물에서 식물 병원성 해충 및 선충으로 인한 작물 생산량 감소 등의 경제적 손실이 심각하다. 우리나라의 경우 시설 재배지의 연작으로 인해 해충과 선충의 발생이 증가하여 작물의 피해가 점차 증가하고 있는 추세다. 현재 주로 사용되고 있는 식물병원성 해충 및 선충의 방제법은 사용이 편리하고 비용이 저렴한 화학적 방제법을 이용하고 있지만 화학 농약의 남용으로 인한 약제 저항성이 발생하고, 화학 약품으로 인한 인체 유해성 및 환경오염 문제가 대두되고 있다. 이로 인해 최근 친환경적인 방제에 관한 지속적인 연구와 관심이 높아지면서 식물병원성 해충과 선충에 대하여 살충/살선충 활성을 나타내는 미생물을 생물학적 방제법으로 이용하기 위한 시도가 증가하고 있다. 본 리뷰에서는 식물병원성 해충과 선충에 대한 살충/살선충 활성을 나타내는 세균, 진균, 바이러스에 의한 살충 및 살선충 사례와 기전들을 소개하였다. 이러한 미생물의 활성은 외피 분해효소와 독소물질생산을 통한 직접적인 작용기전과 기주 식물의 저항성 유도를 통한 간접적인 작용기전에 의해서 일어난다. 본 리뷰를 통하여 선충과 해충에 대한 생물학적 방제법과 그 분자 작용기전에 대한 이해를 돕고, 최신 살충 및 살선충 연구들을 소개함으로써 국내의 농민들과 연구자들의 미생물을 기반으로 한 친환경적 해충 및 선충의 방제법에 대한 저변확대를 기대한다. World-wide crop loss caused by insect pest and nematode reaches critical level. In Korea, similar crop loss has been gradually augmented in the field and greenhouse due to continuous crop rotation. The current methods on controlling herbivorous insects and plant parasitic nematodes are mostly depended on agro-chemicals that have resulted additional side-effect including occurrence of resistant insects and nematodes, environmental contamination, and accumulation in human body. To overcome the pitfalls, microbe-based control method have been introduced and applied for several decades. Here, we revised biological control using by the bacteria, fungi, and virus in order to kill insect and nematode and to attenuate its virulence mechanism. The introduced microbes mainly secreted out the hydrolysing enzymes and toxic compounds to target host membrane or cell wall directly. Indirectly, the microbe-triggered plant innate immunity against insects and nematodes was also reported. In conclusion, we provide a new frontier of microbe-based environmentally friendly procedure and effective methods to manage insects and nematodes.