Is there any Relationship between Food Habits in the Last Two Decades and Gastric Cancer in North-western Iran?

Somi, Mohammad Hossein,Mousavi, Seyed Mohsen,Naghashi, Shahnaz,Faramarzi, Elnaz,Jafarabadi, Mohammad Asghari,Ghojazade, Morteza,Majidi, Alireza,Alavi, Seyed Ahmad Naseri Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Purpose: The aims of this case-control study were to assess the correlation between some food habits in the last two decades and gastric cancer in East Azerbaijan of Iran. Materials and Methods: In this hospital based case control study, 616 patients (212 gastric cancer patients, 404 cancer free patients) were recruited. Food habits of patients over the past two decades were assessed with a structured questionnaire. We used conditional logistic regression analysis for estimating crude and adjusted odds ratios (OR) and their respective 95% confidence intervals (95%CI). Results: In this study, over-eating, consumption of high fat milk and yogurt and especial types of cheese increased the risk of gastric cancer (All<0.05). Consumption of such especial cheeses such as Koze and Khiki increased the risk of gastric cancer by 12.6 fold (95% CI:1.99-79.36) and 7.36 fold (95% CI:1.33-40.54), respectively. In addition, high fat food, moldy food, and pickled vegetables consumption as well as reuse of cooking oil for frying were significantly associated with gastric cancer risk. Furthermore, intake of Ghorme (deep fried meat) was positively correlated with gastric cancer risk (OR:1.31;95%CI: 0.91-1.87). Conclusions: It can be confirmed that particular food habits which have been very common in East-Azerbaijan in the last two past decades increase risk of gastric cancer. According to our results and taking into account the long latency period of gastric cancer it can be concluded that nutrition education for a healthy diet should be performed from early childhood. However, further well designed cohort studies are needed to achieve more clear results.

Study of QTLs linked to awn length and their relationships with chloroplasts under control and saline environments in bread wheat

Bahram Masoudi,Mohsen Mardi,Eslam Majidi Hervan,Mohammad Reza Bihamta,Mohammad Reza Naghavi,Babak Nakhoda,Behnam Bakhshi,Mehrzad Ahmadi,Mohammad Taghi Tabatabaei,Mohamad Hossein Dehghani Firouzabadi 한국유전학회 2019 Genes & Genomics Vol.41 No.2

Introduction Some studies in wheat showed that awns may have a useful effect on yield, especially under drought stress. Up to this time few researches has identified the awn length QTLs with different effect in salinity stress. Objective The primary objective of this study was to examine the additive (a) and the epistatic (aa) QTLs involve in wheat awns length in control and saline environments. Methods A F7 RIL population consisting of 319 sister lines, derived from a cross between wheat cultivars Roshan and Falat (seri82), and the two parents were grown in two environments (control and Saline) based on an alpha lattice design with two replications in each environment. At flowering, awn length was measured for each line. For QTL analysis, the linkage map of the ‘‘Roshan × Falat’’ population was used, which included 748 markers including 719 DArT, 29 simple sequenced repeats (SSRs). Additive and pleiotropic QTLs were identified. In order to reveal the relationship between the identified QTL for awns length and the role of the gene or genes that it expresses, the awns length locus location and characteristics of its related CDS, gene, UTRs, ORF, exons and Introns were studied using ensemble plant (http://plant s.ensem bl.org/Triti cum_aesti vum). Furthermore, the promoter analysis has been done using NSITE-PL. Results We identified 6 additive QTLs for awn length by QTL Cartographer program using single-environment phenotypical values. Also, we detected three additive and two epistatic QTLs for awn length by the QTLNetwork program using multienvironment phenotypical values. Our results showed that none of the additive and epistatic QTLs had interactions with environment. One of the additive QTLs located on chromosome 4A was co-located with QTLs for number of sterile spikelet per spike in both environment and number of seed per spike in control environment. Coclusion Studies of the locus linked to the awns length QTL revealed the role of awn and its chloroplasts in grain filing during abiotic stress could be enhanced by over expression of some genes like GTP-Binding proteins which are enriched in chloroplasts encoded by genes included wPt-5730 locus.

Assessment of the Allelopathic Potential of Cumin Accessions in Different Soil Water Potential

Zahra Ghafari,Hassan Karimmojeni,Mohammad M. Majidi,Bahare Naderi 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.3

Allelopathy is one effective solution to reach a healthy life without any pollution. This study was carried out to evaluate the allelopathic potential of 92 Iranian cumin accessions grown under normal conditions and drought stress, according to the sandwich method in the laboratory. In addition, weed infestation of the same cumin accessions was examined in a field by measuring total weed species richness and diversity under normal irrigating conditions. Results indicated that length and fresh weight of radish radicle and hypocotyl are more sensitive than the other germination indices against exuded allelochemicals of cumin seeds. Furthermore, all genotypes showed the average germination percentage to be less than the control samples. They could significantly reduce total species richness and diversity in their surroundings. Weed suppressive percentage of cumin genotypes in the field experiments was positively correlated with inhibition percentage of radicle growth in the laboratory. Water stress increases the allelopathic effects of cumin accessions in laboratory screening. Classification based on principle component method indicated that cumin genotypes were categorized into two clusters in both moisture regimes. Despite having stress factors, only the genotypes 13CGOGK1, 35CSESH1, 65CEAAS, 68CKERF2, 75CYAAR2, and 84CKRTJ2 remained in a group with strong allelopathic activity. According to these results, there is the significant diversity for allelopathic strength in the Iranian cumin germplasm, which can be useful in cumin breeding for weed suppression.

Silibinin- Induced Apoptosis and Downregulation of MicroRNA-21 and MicroRNA-155 in MCF-7 Human Breast Cancer Cells

Masoud Maleki Zadeh,Nasrin Motamed,Najmeh Ranji,Mohammad Majidi,Fahimeh Falahi 한국유방암학회 2016 Journal of breast cancer Vol.19 No.1

Purpose: MicroRNAs (miRNAs) have received much attention owing to their aberrant expression in various stages of cancer. In many biological processes, miRNAs negatively regulate gene expression, and may be useful in therapeutic strategies. The present study evaluated the effects of silibinin (silybin), a natural flavonoid, on miRNA expression and attempted to elucidate therapeutic targets in MCF-7 breast cancer cells. Methods: The rates of cell proliferation and apoptosis were determined in silibinintreated and untreated MCF-7 cells. Furthermore, the expression levels of miR-21 and miR-155 were measured in MCF-7 cells after incubation with silibinin (100 μg/mL), and the putative targets of the miRNAs within the apoptotic pathways were predicted using bioinformatic approaches. The expression levels of some of these targets were evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: Silibinin induced apoptosis in MCF-7 cells in a dose- and time-dependent manner. qRT-PCR analysis revealed a decrease in miR-21 and miR- 155 expression levels in silibinin-treated cells relative to the levels in the untreated cells. Potential miR-21 and miR-155 targets within the apoptotic pathways, such as CASP-9, BID, APAF-1, CASP-3, CASP-8, and PDCD4, were predicted by in silico analysis. qRT-PCR analysis showed upregulation of some of these potential targets including caspase-9 (CASP-9) and BID after silibinin treatment for 48 hours. Conclusion: Our results suggest a correlation between the expression of miR-21 and miR-155, and MCF-7 cell proliferation. The antiproliferative activity of silibinin may partly be attributable to the downregulation of miR-21 and miR-155, and the upregulation of their apoptotic targets. Furthermore, the upregulation of CASP-9 and BID indicates that silibinin induces apoptosis through both the extrinsic and intrinsic pathways.

Experimental measurement and modeling of saturated reservoir oil viscosity

Abdolhossein Hemmati-Sarapardeh,Amir H. Mohammadi,Ahmad Ramazani S. A.,Seyed-Mohammad-Javad Majidi,Behnam Mahmoudi 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.7

A novel mathematical-based approach is proposed to develop reliable models for prediction of saturatedcrude oil viscosity in a wide range of PVT properties. A new soft computing approach, namely least square supportvector machine modeling optimized with coupled simulated annealing optimization technique, is proposed. Six modelshave been developed to predict saturated oil viscosity, which are designed in such a way that could predict saturatedoil viscosity with every available PVT parameter. The constructed models are evaluated by carrying out extensive experimentalsaturated crude oil viscosity data from Iranian oil reservoirs, which were measured using a “Rolling Ballviscometer.” To evaluate the performance and accuracy of these models, statistical and graphical error analyses wereused simultaneously. The obtained results demonstrated that the proposed models are more robust, reliable and efficientthan existing techniques for prediction of saturated crude oil viscosity.