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- 저자 : Markakis, Pericles (검색결과 4 건)
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Aspergillus parasiticus 에 의한 보리의 Aflatoxin 생성과 감마선의 영향
장학길,Markakis, Pericles 한국균학회 1981 韓國菌學會誌 Vol.9 No.1
The effect of gamma irradiation on production and accumulation of affatoxin on natural substrate (barley) by Aspergillus parasiticus NRRL 2999 has been studied in some detail_ Gamma irradiation at five doses, 0. 50, 100, 200 and 400 Krad was applied to the grain either soon after moisture equilibration (3 days after inoculation) or 10 days later (13 days after inoculation). And the results were as in the followings. 1. Increase in moisture content from 17% to 25% greatly increased the aflatoxin concentration, especially at zero irradiation dose. 2. Prolongation of the incubation period prior to irradiation from 3 to 13 days resulted in greater accumulation of aflatoxin. 3. Two hundreds Krad applies 13 days after inoculation on barley stored at 25% moisture (100% RH) and 25℃ led to higher aflatoxin production than 100 Krad or even 50 Krad. 4. The relative proportion of the principal aflatoxins in relation to irradiation showed that aflatoxin G was elaborated at a significantly higher rate than aflatoxin B.
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보리의 Aspergillus parasiticus 감수성
김창식,장학길,Markakis, Pericles 한국균학회 1982 韓國菌學會誌 Vol.10 No.2
The seeds of 20 barley cultivars were tested for aflatoxin contamination and susceptibility to infection by an aflatoxin-producing mold. When the samples were tested as they arrived, no aflatoxin was detected on any of them. When their moisture was raised to 25% and they were kegt as 25℃ for 2 weeks, all expect 2 cultivars showed aflatoxin contamination. Aflatoxins B₂ and G₂ were not detected in this incubation period. After wetting (25% moisture) the samples, inoculating them with Aspergillus parasiticus conidia and storing them at 25℃ for 2 weeks, all cultivars were found heavily contaminated with aflatoxin, those with seedcoats more so than those without seedcoats.
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Spear, Rose L.,Symeonidou, Antonia,Skepper, Jeremy N.,Brooks, Roger A.,Markaki, Athina E. Techno-Press 2015 Biomaterials and biomedical engineering Vol.2 No.3
Successful integration of cementless femoral stems using porous surfaces relies on effective periimplant bone healing to secure the bone-implant interface. The initial stages of the healing process involve protein adsorption, fibrin clot formation and cell osteoconduction onto the implant surface. Modelling this process in vitro, the current work considered the effect of fibrin deposition on the responses of human mesenchymal stromal cells cultured on ferritic fibre networks intended for magneto-mechanical actuation of in-growing bone tissue. The underlying hypothesis for the study was that fibrin deposition would support early stromal cell attachment and physiological functions within the optimal regions for strain transmission to the cells in the fibre networks. Highly porous fibre networks composed of 444 ferritic stainless steel were selected due to their ability to support human osteoblasts and mesenchymal stromal cells without inducing untoward inflammatory responses in vitro. Cell attachment, proliferation, metabolic activity, differentiation and penetration into the ferritic fibre networks were examined for one week. For all fibrin-containing samples, cells were observed on and between the metal fibres, supported by the deposited fibrin, while cells on fibrin-free fibre networks (control surface) attached only onto fibre surfaces and junctions. Initial cell attachment, measured by analysis of deoxyribonucleic acid, increased significantly with increasing fibrinogen concentration within the physiological range. Despite higher cell numbers on fibrin-containing samples, similar metabolic activities to control surfaces were observed, which significantly increased for all samples over the duration of the study. It is concluded that fibrin deposition can support the early attachment of viable mesenchymal stromal cells within the inter-fibre spaces of fibre networks intended for magneto-mechanical strain transduction to in-growing cells.
ScienceON
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