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Nadalizadeh Ghannad Atefeh,Marashi Hassan,Seifi Alireza,Moshiri Fereshte 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5
This study aimed to investigate the callus induction and shoot regeneration to produce rootstock from stems and leaves explants of the Ohadi and UCB-1 pistachio cultivars. Plant growth regulators (PGRs), including combinations of BAP, Kin, IAA, NAA, and 2, 4-D, were added to Murashige and Skoog (MS) or Driver and Kuniyuki Walnut (DKW) media to induce callus induction and regeneration. Obtained calli were transferred to the DKW medium containing three different concentrations, 2 and 4 mg L−1 of BAP and 1 mg L−1 + 2 mg L−1 of BAP + NAA, to compare the frequency of shoot regeneration. The highest callus induction rate (96%) was observed in the DKW medium supplemented with 2 mg L−1 Kin + 0.5 mg L−1 NAA in the Ohadi stems. The highest callus induction frequency for leaves was obtained in the DKW medium supplemented with 2 mg L−1 BAP + 0.1 mg L−1 2, 4-D concentrations for both UCB-1 (73%) and Ohadi cultivar (71%). The highest shoot regeneration rate in Ohadi stems (41%) and leaves (20%) was observed in the medium supplemented with 2 mg L−1 NAA + 1 mg L−1 BAP. It resulted in significant differences (P ≤ 0.05) in callus induction and shoot regeneration between stems and leaves; stems generated more calli and shoots than leaves, and Ohadi significantly (P ≤ 0.05) inducted calli and generated shoots more than UCB-1. Altogether, these regeneration protocols, achieved from stems and leaves of the Ohadi and UCB-1 cultivars, may be helpful in future pistachio genetic transformation.
Rezaei-Tavirani, Mostafa,Moghaddamnia, Seyed Hassan,Ranjbar, Bijan,Amani, Mojtaba,Marashi, Sayed-Amir Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.5
Thermal conformational changes of human serum albumin (HSA) in phosphate buffer, 10 mM at pH = 7 are investigated using differential scanning calorimetric (DSC), circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature increment from $25^{\circ}C$ to $55^{\circ}C$ induces reversible conformational changes in the structure of HSA. Conformational change of HSA are shown to be a three-step process. Interestingly, melting temperature of the last domain is equal to the maximum value of fever in pathological conditions, i.e. $42^{\circ}C$. These conformational alterations are accompanied by a mild alteration of secondary structures. Study of HSA-SDS (sodium dodecyl sulphate) interaction at $45^{\circ}C$ and $35^{\circ}C$ reveals that SDS affects the HSA structure at least in three steps: the first two steps result in more stabilization and compactness of HSA structure, while the last one induces the unfolding of HSA. Since HSA has a more affinity for SDS at $45^{\circ}C$ compared to $35^{\circ}C$, It is suggested that the net negative charge of HSA is decreased in fever, which results in the decrease of HSA-associated cations and plasma osmolarity, and consequently, heat removal via the increase in urine volume.