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- 저자 : Manuelle Bodin (검색결과 2 건)
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Enhancement of embryo yield from isolated microspores of Brassica napus by early iron starvation
Benoit Jacques Gabriel Leroux,Philippe Potin,Francois Robert Larher,Manuelle Bodin 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.6
Induction of embryogenesis in isolated microspores of Brassica napus requires stress conditions to trigger the developmental instead of the gametophytic pathway. To obtain further insight into the involvement of different ions in this process, a comparison has been made between embryo yields obtained with standard NLN-13 medium and the same medium without cobalt, copper or iron. It was confirmed that iron was essential to control embryo development, but not cobalt and copper. For the latter two ions, the concentration is probably too low to play a significant role in microspore embryogenesis. With the timing of iron application, as well as its chemical form, embryo yield could be improved or reduced. In media that exhibited iron deficiency, microspores initiated embryogenesis and the number of observed divided microspores increased 6 days after isolation. However, embryo development was not achieved. Addition of iron ions chelated with EDTA at day 3, leading to the doubling of embryo yield. Some of the putative role(s) of Fe-EDTA in the early events of embryogenesis is discussed.
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Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus
Benoıˆt Leroux,Nathalie Carmoy,Delphine Giraudet,Philippe Potin,Franc¸ois Larher,Manuelle Bodin 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4
Procedures that induce microspore embryogenesis have been described for a range of Brassica species, but embryo yield remains low for a number of genotypes. We have carried out experiments with the microspores from a weakly responsive line of B. napus to determine the culture conditions that optimize their in vitro embryogenesis by treating them with effectors of ethylene synthesis and action. The results revealed that isolated microspores subjected to an initial heat stress in a medium supplemented with inhibitors of ethylene synthesis such as AVG and CoCl2 exhibited significantly increased embryo yields. This suggested that regulatory effects are exerted by the ethylene produced by the isolated microspores on the early processes of gametogenesis. As a consequence, treatment of microspores with SAM, an ethylene synthesis precursor, or with the ethylene-releasing agent ethephon, led to decreases in embryo yield. A special response to ethylene during the early stages of microspore development was finally shown to occur through experiments where isolated microspores were treated for increasing periods of time with CoCl2. Collectively, our data demonstrated that the induction of embryogenesis induced by heat stress can be enhanced by inhibitors of ethylene biosynthesis. Procedures that induce microspore embryogenesis have been described for a range of Brassica species, but embryo yield remains low for a number of genotypes. We have carried out experiments with the microspores from a weakly responsive line of B. napus to determine the culture conditions that optimize their in vitro embryogenesis by treating them with effectors of ethylene synthesis and action. The results revealed that isolated microspores subjected to an initial heat stress in a medium supplemented with inhibitors of ethylene synthesis such as AVG and CoCl2 exhibited significantly increased embryo yields. This suggested that regulatory effects are exerted by the ethylene produced by the isolated microspores on the early processes of gametogenesis. As a consequence, treatment of microspores with SAM, an ethylene synthesis precursor, or with the ethylene-releasing agent ethephon, led to decreases in embryo yield. A special response to ethylene during the early stages of microspore development was finally shown to occur through experiments where isolated microspores were treated for increasing periods of time with CoCl2. Collectively, our data demonstrated that the induction of embryogenesis induced by heat stress can be enhanced by inhibitors of ethylene biosynthesis.
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