Prognostic Role of MicroRNA-21 in Non-small Cell Lung Cancer: a Meta-analysis

Ma, Xue-Lei,Liu, Lei,Liu, Xiao-Xiao,Li, Yun,Deng, Lei,Xiao, Zhi-Lan,Liu, Yan-Tong,Shi, Hua-Shan,Wei, Yu-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Introduction: Many studies have reported that microRNA-21 (miR-21) mihght predict the survival outcome in non-small cell lung cancers (NSCLCs) but the opposite opinion has also been expressed. The aim of this study was to summarize the evidence for a prognostic role of miR-21. Materials and Methods: All the eligible studies was searched by Medline and EMBASE and patients' clinical characteristics and survival outcome were extracted. Then a meta-analysis was performed to clarify the prognostic role of the miR-21 expression in different subgroups. Results: A total of 8 eligible articles were yielded covering survival outcomes or clinical characteristics. The combined hazard ratio (HR) and 95% confidence interval (95% CI) for overall survival (OS) was 2.19 [0.76, 6.30], while the combined HR (95% CI) of Asian group for OS had a significant result, 5.49 [2.46, 12.27]. The combined HR (95% CI) for recurrence free survival or disease free survival (RFS/DFS) was 2.31 [1.52, 3.49]. Odds ratios (ORs) showed that the miR-21 expression was associated with lymph node status and histological type. Conclusion: miR-21 expression could predict the prognostic outcome of NSCLC in Asians, despite some deficiencies in the study data.

An Improved K-means Algorithm based on Mapreduce and Grid

Li Ma,Lei Gu,Bo Li,Yue Ma,Jin Wang 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1

The traditional K-means clustering algorithm is difficult to initialize the number of clusters K, and the initial cluster centers are selected randomly, this makes the clustering results very unstable. Meanwhile, algorithms are susceptible to noise points. To solve the problems, the traditional K-means algorithm is improved. The improved method is divided into the same grid in space, according to the size of the data point property value and assigns it to the corresponding grid. And count the number of data points in each grid. Selecting M(M>K) grids, comprising the maximum number of data points, and calculate the central point. These M central points as input data, and then to determine the k value based on the clustering results. In the M points, find K points farthest from each other and those K center points as the initial cluster center of K-means clustering algorithm. At the same time, the maximum value in M must be included in K. If the number of data in the grid less than the threshold, then these points will be considered as noise points and be removed. In order to make the improved algorithm can adapt to handle large data. We will parallel the improved k-mean algorithm and combined with the MapReduce framework. Theoretical analysis and experimental results show that the improved algorithm compared to the traditional K-means clustering algorithm has high quality results, less iteration and has good stability. Parallelized algorithm has a very high efficiency in data processing, and has good scalability and speedup.

Weighted H∞ Control of Singularly Perturbed Switched Systems with Mode-dependent Average Dwell Time

Lei Ma,Jing Xu,Chenxiao Cai 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.10

In this paper, disturbance attenuation problem of singularly perturbed switched systems is investigated by using a mode-dependent average dwell time (MDADT) approach. By constructing a full-order piecewise Lyapunov function, which depends on the singular perturbation parameter, sufficient conditions to guarantee the exponential stability and weighed H∞ performance are obtained with different singular perturbation parameters and stability bounds of subsystems. Then, theoretical results on the analysis of stability and weighted H∞ performance are derived for a special case that all subsystems have a unified singular perturbation parameter. Thirdly, state feedback controller design methods are proposed. Finally, two examples are given to demonstrate the validity and effectiveness of the proposed methods.

Cockroach as a Vector of Blastocystis sp. is Risk for Golden Monkeys in Zoo

Lei Ma,Yongbin Zhang,Haixia Qiao,Shuai Li,Heqin Wang,Ningning Zhang,Xiao Zhang 대한기생충학열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.5

Blastocystis sp. is a kind of protozoa living in the intestinal tract of human and animals, which will cause intestinal diseases such as diarrhea, abdominal distension and vomiting. This paper was aimed to understand the infection of Blastocystis sp. In golden monkeys and the transmission path in North China. Thirty-seven feces samples from golden monkeys and 116 cockroach samples from Shijiazhuang Zoo were collected from July to October 2019 for PCR analysis of Blastocystis sp. Genetic diversity analysis was further conducted on the samples with positive PCR results. The results showed that the infection rate was 48.7% (18/37) in golden monkeys and 82.8% (96/116) in cockroaches, respectively. The genetic evolution analysis based on small subunit ribosomal RNA demonstrated that three subtypes (ST) of Blastocystis sp. including ST1, ST2, and ST3 existed in the intestinal tract of golden monkeys, while only ST2 was detected in the intestinal tract of cockroaches. This paper may provide supports for the quarantine and control of Blastocystis sp. for the zoo in Northern China.

Assessment of cryopreserved sperm functions of Korean native brindled cattle (Chikso) from different region research centers of Korea

Ma, Lei,Jung, Dae-Jin,Jung, Eun-Ju,Lee, Woo-Jin,Hwang, Ju-Mi,Bae, Jeong-Won,Kim, Dae-Hyun,Yi, Jun Koo,Lee, Sang Moo,Ha, Jae Jung,Kwon, Woo-Sung The Korean Society of Animal Reproduction and Biot 2021 한국동물생명공학회지 Vol.36 No.2

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

Hydrogeochemical characteristic evaluation and irrigation suitability assessment of shallow groundwater in Dangshan County, China

Lei Ma,Tingwei Huang,Han Qiu,Zhangxian Yang,Xiaorui He,Jiazhong Qian 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.5

The current study aims to identify the hydrogeochemical characteristics, formation mechanism and evolution law of shallow groundwater in Dangshan, China, and to evaluate the suitability of the groundwater for irrigation. In this study, ninety-six samples were taken to investigate the hydrogeochemical characteristics of shallow groundwater in Dangshan County. The geographic information system (GIS) method was used to analyse the spatial distribution characteristics of total dissolved solids (TDS). Gibbs diagram, Binary relation analysis and reverse geochemical reaction simulation method were utilised to reveal the main formation mechanism of relatively high TDS in this region. The results show that water-rock interaction and evapotranspiration are the main mechanisms for controlling the chemical evolution of shallow groundwater in wet and dry seasons in the study area, respectively. The main waterrock interactions in the study area include dissolution of halite and dolomite, the precipitation of gypsum, as well as cation exchange and adsorption. Suitability of shallow groundwater quality for irrigation use was evaluated according to the sodium adsorption ratio (SAR) and the electrical conductivity (EC) with a United States Salinity Laboratory (USSL) diagram. Meanwhile, GIS technology was used to demonstrate the spatial distribution of irrigation suitability variations. Based on the spatial interpolation result on the USSL salinity diagram classification, the shallow groundwater in 89% of the study area is suitable for irrigation, while 11% of the area is unsuitable for irrigation.

Costunolide suppresses colorectal cancer cells growth and induces apoptosis in vitro and in vivo

Lei Ma,Myoung Ok Kim 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Assessment of cryopreserved sperm functions of Korean native brindled cattle (Chikso) from different region research centers of Korea

Lei Ma,Dae-Jin Jung,Eun-Ju Jung,Woo-Jin Lee,Ju-Mi Hwang,Jeong-Won Bae,Dae-Hyun Kim,Jun Koo Yi,Sang Moo Lee,Jae Jung Ha,Woo-Sung Kwon 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.2

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

Suppression of transient receptor potential melastatin 7 affects pluripotency, proliferation, and differentiation of mouse embryonic stem cells through mechanistic target of rapamycinextracellular signal-regulated kinase activation

Lei Ma,Zae Young Ryoo,Myoung Ok Kim 한국실험동물학회 2023 한국실험동물학회 학술발표대회 논문집 Vol.2023 No.2

Prognostic Role of Circulating Tumor Cells in Patients with Pancreatic Cancer: a Meta-analysis

Ma, Xue-Lei,Li, Yan-Yan,Zhang, Jing,Huang, Jing-Wen,Jia, Hong-Yuan,Liu, Lei,Li, Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Isolation and characterization of circulating tumor cells (CTCs) in patients suffering from a variety of different cancers have become hot biomarker topics. In this study, we evaluated the prognostic value of CTCs in pancreatic cancer. Materials and Methods: Initial literature was identified using Medline and EMBASE. The primary data were hazard ratios (HRs) with 95% confidence intervals (CIs) of survival outcomes, including overall survival (OS) and progression free survival/recurrence free survival (PFS/RFS). Results: A total of 9 eligible studies were included in this meta-analysis, published between 2002 and 2013. The estimated pooled HR and 95%CI for OS for all studies was 1.64 (95%CI 1.39-1.94, p<0.00001) and the pooled HR and 95%CI for RFS/DFS was 2.36 (95%CI 1.41-3.96, p<0.00001). The HRs and 95%CIs for OS and RFS/DFS in patients before treatment were 1.93 (95%CI 1.26-2.96, p=0.003) and 1.82 (95%CI 1.22-2.72, p=0.003), respectively. In patients receiving treatment, the HRs and 95%CI for OS and RFS/DFS were 1.37 (95%CI 1.00-1.86, p=0.05) and 1.89 (95%CI 1.01-3.51, p=0.05), respectively. Moreover, the pooled HR and 95%CI for OS in the post-treatment group was 2.20 (95%CI 0.80-6.02, p=0.13) and the pooled HR for RFS/DFS was 8.36 (95%CI 3.22-21.67, p<0.0001). Conclusions: The meta-analysis provided strong evidence supporting the proposition that CTCs detected in peripheral blood have a fine predictive role in pancreatic patients especially on the time point of post-treatment.