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Roh, Kyoungmin,Kim, Dong-Min,Lee, Eun Hee,Kim, Hyoseon,Park, Hyung Soon,Jang, Ja-Hyun,Hwang, Sang-Hyun,Kim, Dong-Eun The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.32
<P>We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5′→ 3′ exonuclease activity of <I>Taq</I> polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.</P> <P>Graphic Abstract</P><P>We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc00263j'> </P>
Transition Metal Vinylidene- and Allenylidene-Mediated Catalysis in Organic Synthesis
Roh, Sang Weon,Choi, Kyoungmin,Lee, Chulbom American Chemical Society 2019 Chemical reviews Vol.119 No.6
<P>With their mechanistic novelty and various modalities of reactivity, transition metal unsaturated carbene (alkenylidene) complexes have emerged as versatile intermediates for new reaction discovery. In particular, the past decade has witnessed remarkable advances in the chemistry of metal vinylidenes and allenylidenes, leading to the evolution of a diverse array of new catalytic transformations that are mechanistically distinct from those developed in the previous two decades. This review aims to provide a survey of the recent achievements in the development of organic reactions that make use of transition metal alkenylidenes as catalytic intermediates and their applications to organic synthesis.</P> [FIG OMISSION]</BR>
Yeo, Youn Jee,Roh, Kyoungmin,Bang, Joo Young,Lee, Eun Hee,Park, Hyung Soon,Kim, Dong-Eun The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.36
<P>Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.</P> <P>Graphic Abstract</P><P>Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates, and the PNA probes were quantitatively analyzed with MALDI-TOF mass spectrometry. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc00070b'> </P>
Lee, Jong Seok,Krause, Roland,Schreiber, Jö,rg,Mollenkopf, Hans-Joachim,Kowall, Jane,Stein, Robert,Jeon, Bo-Young,Kwak, Jeong-Yeon,Song, Min-Kyong,Patron, Juan Pablo,Jorg, Sabine,Roh, Kyoungmin,Ch Elsevier 2008 Cell host & microbe Vol.3 No.2
<P><B>Summary</B></P><P>Attenuated strains of mycobacteria can be exploited to determine genes essential for their pathogenesis and persistence. To this goal, we sequenced the genome of H37Ra, an attenuated variant of <I>Mycobacterium tuberculosis</I> H37Rv strain. Comparison with H37Rv revealed three unique coding region polymorphisms. One polymorphism was located in the DNA-binding domain of the transcriptional regulator PhoP, causing the protein's diminished DNA-binding capacity. Temporal gene expression profiles showed that several genes with reduced expression in H37Ra were also repressed in an H37Rv phoP knockout strain. At later time points, genes of the dormancy regulon, typically expressed in a state of nonreplicating persistence, were upregulated in H37Ra. Complementation of H37Ra with H37Rv <I>phoP</I> partially restored its persistence in a murine macrophage infection model. Our approach demonstrates the feasibility of identifying minute but distinct differences between isogenic strains and illustrates the consequences of single point mutations on the survival stratagem of <I>M. tuberculosis.</I></P>