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Jae Youn Hwang,Changyang Lee,Kwok Ho Lam,Hyung Ham Kim,Jungwoo Lee,Shung, K. Kirk IEEE 2014 and Frequency Control Vol.61 No.3
<P>The measurement of cell mechanics is crucial for a better understanding of cellular responses during the progression of certain diseases and for the identification of the cell's nature. Many techniques using optical tweezers, atomic force microscopy, and micro-pipettes have been developed to probe and manipulate cells in the spatial domain. In particular, we recently proposed a two-dimensional acoustic trapping method as an alternative technique for small particle manipulation. Although the proposed method may have advantages over optical tweezers, its applications to cellular mechanics have not yet been vigorously investigated. This study represents an initial attempt to use acoustic tweezers as a tool in the field of cellular mechanics in which cancer cell membrane deformability is studied. A press-focused 193-MHz single-element lithium niobate (LiNbO<SUB>3</SUB>) transducer was designed and fabricated to trap a 5-μm polystyrene microbead near the ultrasound beam focus. The microbeads were coated with fibronectin, and trapped before being attached to the surface of a human breast cancer cell (MCF-7). The cell membrane was then stretched by remotely pulling a cell-attached microbead with the acoustic trap. The maximum cell membrane stretched lengths were measured to be 0.15, 0.54, and 1.41 μm at input voltages to the transducer of 6.3, 9.5, and 12.6 V<SUB>pp</SUB>, respectively. The stretched length was found to increase nonlinearly as a function of the voltage input. No significant cytotoxicity was observed to result from the bead or the trapping force on the cell during or after the deformation procedure. Hence, the results convincingly demonstrated the possible application of the acoustic trapping technique as a tool for cell manipulation.</P>
Yana Li,Xianhua Hou,Yajie Li,Qiang Ru,Shaofeng Wang,Shejun Hu,Kwok-ho Lam 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.5
Hierarchical CoMn2O4 microspheres assembled bynanoparticles have been successfully synthesized by afacile hydrothermal method and a subsequent annealingtreatment. XRD detection indicate the crystal structure. SEM and TEM results reveal the 3-dimensional porousand micro-/nanostructural microsphere assembled bynanoparticles with a size of 20-100 nm. The CoMn2O4electrode show initial specific discharge capacity ofapproximately 1546 mAh/g at the current rates 100 mA/gwith a coulombic efficiency of 66.7% and remarkablespecific capacities (1029-485 mAh/g) at various currentrates (100-2800 mA/g).