Variation in seedling root traits in wild barley (<i>Hordeum vulgare</i> L. ssp. <i>spontaneum</i>) germplasm

Tyagi, Kuldeep,Lee, Hyo Jeong,Lee, Chong Ae,Steffenson, Brian J.,Kim, Young Jin,Yun, Song Joong Cambridge University Press 2014 Plant genetic resources Vol.12 No.1

<P>Improved root architecture of cultivated barley can improve crop performance in drought-prone areas. In this study, seedlings of 315 wild barley (<I>Hordeum vulgare</I> subsp. <I>spontaneum</I>) accessions from the Wild Barley Diversity Collection (WBDC) were grown under hydroponic conditions for 8 d after germination and then root characteristics were analysed. Significant differences were observed among the accessions with regard to seminal root number (SRN), root length (RL), specific root length (SRL), root fresh weight and root dry weight (RDW). Principal component analysis explained about 81% of the total variation for ten traits. Principal component (PC) 1, PC2 and PC3 explained about 38, 30 and 13% of the total variation among the accessions. The two most prominent contributors in each PC were RL and SRL, RDW and SRN, and the longitude and latitude of the collection sites, respectively. Accessions WBDC266, WBDC302, WBDC286 and WBDC011 had the longest RL and the highest RDW, specific dry root weight and SRL, respectively. These accessions may be useful genetic resources for the improvement of these root traits in cultivated barley.</P>

Molecular Markers and Their Usefulness in Rice Breeding

( Neeraj Kumar Tyagi ),( Bandarupalli Ramesh ),( Kuldeep Tyagi ) 전북대학교 농업과학기술연구소 2009 농업생명과학연구 Vol.40 No.2

Molecular markers are extensively used for improving and sustaining the rice productivity. A variety of molecular genetic markers, including restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), microsatellites or simple sequence repeats (SSRs), expressed sequence tags (EST) and single nucleotide polymorphism (SNP) have been developed providing new tools for rice breeding. The major advantages of the molecular markers over the other classes of markers are their number is potentially unlimited, spanning across the genome, their expression is unaffected by the environment and their assessment is independent of the stage of plant development. Molecular markers are landmarks in the chromosome maps that can be used to monitor the transfer of specific chromosome segments known to carry useful agronomic traits. Breeders use these molecular markers to increase the precision of selection for the best trait combinations. Molecular markers have large number of applications ranging from diversity analysis to the improvement of rice varieties by marker assisted selection. This review describes the usefulness of some important DNA markers in rice improvement.

Genetic Variability and Character Association Analysis for Yield-Contributing Traits in Wheat (Triticum aestivum L.)

( Ankur Kumar ),( Sudhir Kumar Dhama ),( Kuldeep Tyagi ),( Neeraj Kumar Tyagi ) 전북대학교 농업과학기술연구소 2011 농업생명과학연구 Vol.42 No.1

The present study was conducted to evaluate the genetic variability and correlation of different characters associated with grain yield in wheat. Forty genotypes were grown in randomized block design with three replications and evaluated for twelve yield and yield-related characters. Highly significant differences and adequate genetic variability were observed among the genotypes for all twelve characters. The heritability showed highest value for plant height, days to 50% flowering, days to maturity, peduncle length, 100 grain weight, number of spikelet per spike and number of grains per spikelet. Highest values of genetic advance were obtained in biological yield, plant height, days to maturity, peduncle length and days to 50% flowering. Correlation studies revealed an intensive selection for number of tillers, days to 50% flowering, days to maturity, number of spikelet per spike, number of grains per spikelet and 100 grain weight will contribute to improve the seed yield in wheat. Path analysis showed that maximum direct effect on grain yield per plant was contributed mostly by biological yield, followed by harvest index, spike length, plant height and days to 50% flowering. Principal components analysis (PCA) was performed and the first five principal components explained 75% of the observed variation. PC1 accounted for 23% of variation and showed the largest loading values for number of tiller`s per plant, biological yield and grain yield per plant. PC2 accounted for 17% and associated with plant height, peduncle length and harvest index. PC3 accounted for 13% and related to the days to 50% flowering and days to maturity. PCA analysis showed that wheat genotypes HPB42, UP2123, PBW527 and HD3024 showed good performance among wheat genotypes on the basis of all traits combined. The identified genotypes may be useful for wheat yield improvement.

Comparative Transcriptome Analysis Reveals Differential Response of Phytohormone Biosynthesis Genes in Glumous Flowers of Cold-Tolerant and Cold-Sensitive Rice Varieties Upon Cold Stress at Booting Stage

Myoung Ryoul Park,Ki Young Kim,Kuldeep Tyagi,So Hyeon Baek,Song Joong Yun 한국육종학회 2011 한국육종학회지 Vol.43 No.1

Low temperature stress is one of the major negative factors affecting vegetative and reproductive growth of rice. To better understand responses of rice plants to low temperature we analyzed transcriptome expression patterns in glumous flower of cold-tolerant japonica rice variety, Stejaree45, and cold-susceptible variety, HR19621-AC6 at booting stage under cold water irrigation. A total of 2,411 probes were differentially expressed by low temperature in glumous flowers of the two varieties. Some important genes involved in hormone biosynthesis showed variety-specific regulation. Expression of GA20ox3 and GA2ox, among the genes involved in GA biosynthesis, was regulated differentially in the two varieties. Among the genes involved in IAA biosynthesis, YUCCA1 and TAA1:1 showed variety-specific regulation. Among the genes involved in cytokinin biosynthsis and signaling, expression of LOG, HK1 and HK3 was significantly down-regulated only in the cold-susceptible variety. Among the genes involved in ABA biosynthesis, NSY and AAO3 were down-regulated only in the cold-tolerant variety. In general, genes involved in GA, IAA and cytokinin biosynthesis responded to cold temperature in such a way that capacity of those bioactive hormones is maintained at relatively higher levels under cold temperature in the cold-tolerant variety, which can help minimize cold stress imposed to developing reproductive organs in the cold-tolerant variety.

Rapid Identification and Validation of Xanthomonas oryzae pv. oryzae (Xoo) by using PCR-amplified Phage Integrase and Transposase A Gene

Hyeong-Kwon Shim,Tae-Hwan Noh,Mi-Hyung Kang,Young-Jin Park,Du-Ku Lee,Byoung Moo Lee,Kuldeep Tyagi,Chae-Hoon Paik,Geon-Hwi Lee 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2

벼 흰잎마름병균의 정확한 진단을 위하여 PCR용 진단 kit를 개발하였다. 본 PCR kit를 개발하기 위하여 벼 흰잎마름 병균 유전체 정보 중 phage-related integrase and transposase gene의 염기서열을 이용하여 프라이머를 각각 제작하였다. 프라이머 염기서열은 XOP-F (5-CGG TCT GCT CAA TGA GGA AGA-3)와 XOP-R2 (5-TGC AAT TGG TGT TCTCCA GG-3), XOT-F (5-GTC ATA GGT GAG GCT TCCC-3)와 XOT-R2 (5-AGT GCG ATC TTT CAG CAG G-3)로 벼 흰잎마름병균의 DNA를 401bp와 492bp를 증폭하게 제작하였다. PCR 증폭은 벼 흰잎마름병균만 증폭하였으며 다른 세균인 Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonasputida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoipv. savastanoi and P. marginalis pv. Marginalis 등은증폭되지 않아 특이성이 인정 되었다. 본 프라이머로 병이 의심되는 벼잎과 논물에서 병원균을 3시간 이내에 검출할 수 있었다. PCR-based specific identification of Xanthomonas oryzae pv. oryzae (Xoo), the pathogen responsible for bacterial blight (BB) of rice, was developed by amplifying the Xoo-specific phage-related integrase and transposase gene. Therefore, dual primers, XOP-F (5-CGGTCTGCTCAATGAGGAAGA-3) and XOP-R2 (5-TGCAATTGGTGTTCTCCAGG-3) and XOT-F (5-GTCATAGGTGAGGCTTCCC-3) and XOT-R2 (5-AGTGCGATCTTTCAGCAGG-3) were designed and found to specifically amplify 401bp and 492bp fragments from all strains of Xoo isolates from diverse regions in Korea. The PCR products were only amplified from Xoo among other bacterial strains including Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonas putida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoi pv. savastanoi and P. marginalis pv. marginalis. This method could also be applied to detect the pathogen in infected rice leaves and paddy field water within 3 hr.

연구논문 : 자연과학 ; Phage integrase and transposase A 유전자의 PCR 방법을 이용한 벼 흰잎마름병균의 신속 진단

심형권 ( Hyeong Kwon Shim ),노태환 ( Tae Hwan Noh ),강미형 ( Mi Hyung Kang ),박영진 ( Young Jin Park ),이두구 ( Du Ku Lee ),이병무 ( Byoung Moo Lee ),( Kuldeep Tyagi ),백채훈 ( Chae Hoon Paik ),이건휘 ( Geon Hwi Lee ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2

벼 흰잎마름병균의 정확한 진단을 위하여 PCR용 진단 kit를 개발하였다. 본 PCR kit를 개발하기 위하여 벼 흰잎마름병균 유전체 정보 중 phage-related integrase and transposase gene의 염기서열을 이용하여 프라이머를 각각 제작하였다. 프라 이머 염기서열은 XOP-F (5-CGG TCT GCT CAA TGA GGA AGA-3)와 XOP-R2 (5-TGC AAT TGG TGT TCT CCA GG-3), XOT-F (5-GTC ATA GGT GAG GCT TCC C-3)와 XOT-R2 (5-AGT GCG ATC TTT CAG CAG G-3)로 벼 흰잎마름병균의 DNA를 401bp와 492bp를 증폭하게 제작하였다. PCR 증폭은 벼 흰잎마름병균만 증폭하였으며 다른 세균인 Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonas putida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoi pv. savastanoi and P. marginalis pv. Marginalis 등은 증폭되지 않아 특이성이 인정 되었다. 본 프라이머로 병이 의심되는 벼잎과 논물에서 병원균을 3시간 이내에 검출할 수 있었다. PCR-based specific identification of Xanthomonas oryzae pv. oryzae (Xoo), the pathogen responsible for bacterial blight (BB) of rice, was developed by amplifying the Xoo-specific phage-related integrase and transposase gene. Therefore, dual primers, XOP-F (5-CGGTCTGCTCAATGAGGAAGA-3) and XOP-R2 (5-TGCAATTGGTGTTCTCCAGG-3) and XOT-F (5-GTCATAGGTGAGGCTTCCC-3) and XOT-R2 (5-AGTGCGATCTTTCAGCAGG-3) were designed and found to specifically amplify 401bp and 492bp fragments from all strains of Xoo isolates from diverse regions in Korea. The PCR products were only amplified from Xoo among other bacterial strains including Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonas putida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoi pv. savastanoi and P. marginalis pv. marginalis. This method could also be applied to detect the pathogen in infected rice leaves and paddy field water within 3 hr.