[PD-0019] Expression of colorectal cancer antigenic protein fused to IgM Fc for plant cell factory system using Chinese cabbage (Brassica rapa)

Do-Sun Kim(Do-Sun Kim),Ye-Rin Lee(Ye-Rin Lee),Kisung Ko(Kisung Ko),Taewon Yang(Taewon Yang),Seung-Won Lee(Seung-Won Lee),Sang-Hyun Moh(Sang-Hyun Moh),Hye-Eun Lee(Hye-Eun Lee),Eun Su Lee(Eun Su Lee),Ko 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells without Microenvironment of Feeder Cells

Lee, Seung-Won,Wu, Guangming,Choi, Na Young,Lee, Hye Jeong,Bang, Jin Seok,Lee, Yukyeong,Lee, Minseong,Ko, Kisung,Scholer, Hans R.,Ko, Kinarm Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.7

Spermatogonial stem cells (SSCs) derived from mouse testis are unipotent in regard of spermatogenesis. Our previous study demonstrated that SSCs can be fully reprogrammed into pluripotent stem cells, so called germline-derived pluripotent stem cells (gPS cells), on feeder cells (mouse embryonic fibroblasts), which supports SSC proliferation and induction of pluripotency. Because of an uncontrollable microenvironment caused by interactions with feeder cells, feeder-based SSC reprogramming is not suitable for elucidation of the self-reprogramming mechanism by which SSCs are converted into pluripotent stem cells. Recently, we have established a Matrigel-based SSC expansion culture system that allows longterm SSC proliferation without mouse embryonic fibroblast support. In this study, we developed a new feeder-free SSC self-reprogramming protocol based on the Matrigel-based culture system. The gPS cells generated using a feeder-free reprogramming system showed pluripotency at the molecular and cellular levels. The differentiation potential of gPS cells was confirmed in vitro and in vivo. Our study shows for the first time that the induction of SSC pluripotency can be achieved without feeder cells. The newly developed feeder-free self-reprogramming system could be a useful tool to reveal the mechanism by which unipotent cells are self-reprogrammed into pluripotent stem cells.

Multigenerational effects of maternal cigarette smoke exposure during pregnancy on sperm counts of F1 and F2 male offspring

Lee, Hye Jeong,Choi, Na Young,Park, Yo Seph,Lee, Seung-Won,Bang, Jin Seok,Lee, Yukyeong,Ryu, Jae-Sung,Choi, Seong-Jin,Lee, Sang-Hyub,Kim, Gwang Soo,Chung, Hyun Woo,Ko, Kisung,Lee, Kyuhong,Ko, Kinarm Elsevier 2018 Reproductive toxicology Vol.78 No.-

<P><B>Abstract</B></P> <P>Animal models and human studies showed that <I>in utero</I> cigarette smoke exposure decreases sperm counts of offspring. This study used a mouse model to investigate the effects of maternal exposure to cigarette smoke on reproductive systems in F1 and F2 male offspring. Female ICR mice were exposed either to clean air or to cigarette smoke during pregnancy at the post-implantation stage. Epididymal sperm counts were decreased in a cigarette smoke dose–dependent manner in F1 (by 40–60%) and F2 males (by 23–40%) at postnatal day 56. In F1, the seminiferous epithelium heights were lower in the cigarette smoke–exposed groups than in the control group, and these effects were sustained in F2 males. Results suggest that maternal cigarette smoke exposure during pregnancy can have a multigenerational adverse effect on sperm counts in male offspring, which is mediated through <I>in utero</I> exposure of fetal germ cells to cigarette smoke.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Maternal cigarette smoke exposure caused low sperm counts of male offspring. </LI> <LI> Seminiferous epithelium heights of male offspring were decreased in the exposed groups. </LI> <LI> Maternal cigarette smoke exposure has a multigenerational adverse effect. </LI> </UL> </P>

Expression and in vitro function of anti-PD-L1 human antibody expressed in plant

Lee Chae Eun,Lee Jeong Hwan,Chung Hyun Joo,Lee Da Won,Lim Jong Seok,Kim Kibum,Kim Jin Wook,Lee Yong Seong,Kim Kyung Soo,민현진,Ko Kisung,명순철 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4

Plants can be used to produce recombinant functional proteins such as antibodies and vaccines. In this study, transgenic plants were utilized to express anti-human programmed death ligand-1 (PD-L1) monoclonal antibodies (mAbP PD-L1) tagged with the endoplasmic reticulum retention motif KDEL. PCR and immunoblot analyses confirmed that mAb PD-L1 was expressed in transgenic plants. ELISA analysis confirmed the binding activity of mAbP PD-L1 to human prostate and urothelial cancer cell lines PC-3 and TCCSUP, respectively. Confocal imaging analysis demonstrated that mAbP PD-L1 had binding activity against human cancer cell lines similar to Avelumab (mAbM PD-L1). Confocal imaging analysis showed that the binding activity of mAbP PD-L1 to the human tonsil is comparable to mAbM PD-L1. These results suggest that mAbP PD-L1 shows functional binding activities for PD-L1+ cancer cells. Hence, this study showed that tobacco plants can be used to produce functional anti-human PD-L1 mAbs.

Measurements of Surgeons’ Exposure to Ionizing Radiation Dose During Intraoperative Use of C-Arm Fluoroscopy

Lee, Kisung,Lee, Kyoung Min,Park, Moon Seok,Lee, Boram,Kwon, Dae Gyu,Chung, Chin Youb Lippincott Williams Wilkins, Inc. 2012 1528-1159) Vol.37 No.14

STUDY DESIGN.: Measurement of radiation dose from C-arm fluoroscopy during a simulated intraoperative use in spine surgery. OBJECTIVE.: To investigate scatter radiation doses to specific organs of surgeons during intraoperative use of C-arm fluoroscopy in spine surgery and to provide practical intraoperative guidelines. SUMMARY OF BACKGROUND DATA.: There have been studies that reported the radiation dose of C-arm fluoroscopy in various procedures. However, radiation doses to surgeons’ specific organs during spine surgery have not been sufficiently examined, and the practical intraoperative radioprotective guidelines have not been suggested. METHODS.: Scatter radiation dose (air kerma rate) was measured during the use of a C-arm on an anthropomorphic chest phantom on an operating table. Then, a whole body anthropomorphic phantom was located besides the chest phantom to simulate a surgeon, and scatter radiation doses to specific organs (eye, thyroid, breast, and gonads) and direct radiation dose to the surgeon’s hand were measured using 4 C-arm configurations (standard, inverted, translateral, and tube translateral). The effects of rotating the surgeon’s head away from the patient and of a thyroid shield were also evaluated. RESULTS.: Scatter radiation doses decreased as distance from the patient increased during C-arm fluoroscopy use. The standard and translateral C-arm configurations caused lower scatter doses to sensitive organs than inverted and tube translateral configurations. Scatter doses were highest for breast and lowest for gonads. The use of a thyroid shield and rotating the surgeon’s head away from the patient reduced scatter radiation dose to the surgeon’s thyroid and eyes. The direct radiation dose was at least 20 times greater than scatter doses to sensitive organs. CONCLUSION.: The following factors could reduce radiation exposure during intraoperative use of C-arm; (1) distance from the patient, (2) C-arm configuration, (3) radioprotective equipments, (4) rotating the surgeons’ eyes away from the patient, and (5) avoiding direct exposure of surgeons’ hands.

Expression and function of recombinant anti-colorectal cancer mAb CO17-1A in SfSWT4 insect cells

Se-Ra Park,Kyung Jin Lee,Jeong-Hwan Lee,David Hedin,Thera Mulvania,Seung Ho Lee,Kisung Ko 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Colorectal cancer is the third most commonly diagnosed cancer in the world, nearly all patients diagnosed with this cancer die from it. Antibodies are glycoprotein molecules, which can efficiently recognize and eliminate specific pathogenic and disease antigens. Antibody researches for the last several decades have demonstrated the potential of therapeutic antibodies to fight cancer. Monoclonal antibody (mAb) CO17-1A recognizes the tumor-associated antigen GA733-2, a cell surface glycoprotein highly expressed in colorectal carcinoma cell, which is applicable for preventing and curing colorectal cancer. We have currently established baculovirus insect cell expression system to produce anti-colorectal cancer mAb CO17-1A. In this study, mAb CO17-1A was expressed in the transgenic insect cell line SWT4, which has humanized glycosylation processing pathway. Immunoblot confirmed that mAb CO17-1A properly expressed in SWT4. mAb CO17-1A was purified using protein G affinity column. In addition, Maldi-TOF verified that the mAb fused to KDEL, ER retention signal had high mannose type of glycan structure whereas the mAb without KDEL had partially humanized glycan structure. These results suggest that the insect cell expression system with the SWT4 possibly can be used as a useful alternative way to produce full-size mAb with humanized glycan structures for cancer immunotherapy.

Development of a DAQ system for a plasma display panel-based X-ray detector (PXD)

Lee, Hakjae,Jung, Young-Jun,Eom, Sangheum,Kang, Jungwon,Lee, Kisung Elsevier 2015 Nuclear Instruments & Methods in Physics Research. Vol.784 No.-

<P><B>Abstract</B></P> <P>Recently, a novel plasma display panel (PDP)-based X-ray detector (PXD) was developed. The goal of this study is to develop a data acquisition system for use with the PXD as an imaging detector. Since the prototype detector does not have any barrier ribs or a switching device in a detector pixel, a novel pixelation scheme—the line-scan method—is developed for this new detector. To implement line scanning, a multichannel high-voltage switching circuit and a multichannel charge-acquisition circuit are developed. These two circuits are controlled by an FPGA-based digital signal processing board, from which the information about the charge and position of each pixel can be sent to a PC. FPGA-based baseline compensation and switching noise rejection algorithms are used to improve the signal-to-noise ratio (SNR). The characteristic curve of the entire PXD system is acquired, and the correlation coefficients between the X-ray dose, and the signal intensity and the SNR were determined to be approximately 0.99 and 52.9, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We developed a data acquisition circuit for a novel X-ray imaging detector. </LI> <LI> Line scan, noise rejection, and data transmission methods have been implemented by the FPGA. </LI> <LI> The linearity and SNR of the proposed detector system have been measured quantitatively. </LI> </UL> </P>

Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury

Lee, Yukyeong,Kim, C-Yoon,Lee, Hye Jeong,Kim, Jae Gon,Han, Dong Wook,Ko, Kisung,Walter, James,Chung, Hyung-Min,Schö,ler, Hans R.,Bae, Young Min,Ko, Kinarm Frontiers Media S.A. 2018 Frontiers in cellular neuroscience Vol.12 No.-

<P>Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced reprogramming and differentiation techniques have made it possible to generate therapeutic cells for treating SCI. In the present study, we used directly-induced neural stem cells (DNSCs) from fibroblasts to establish OPCs (DN-OPCs) capable of proliferation and confirmed their OPC-specific characteristics. Also, we evaluated the effect of transplanted DN-OPCs on SCI in rats. The DN-OPCs exhibited an OPC-specific phenotype and electrophysiological function and could be differentiated into oligodendrocytes. In the SCI model, transplanted DN-OPCs improved behavior recovery, and showed engraftment into the host spinal cord with expression of myelin basic protein. These results suggest that DN-OPCs could be a new source of potentially useful cells for treating SCI.</P>

3D Non-Rigid Registration for Abdominal PET-CT and MR Images Using Mutual Information and Independent Component Analysis

Lee, Hakjae,Chun, Jaehee,Lee, Kisung,Kim, Kyeong Min The Institute of Electronics and Information Engin 2015 IEIE Transactions on Smart Processing & Computing Vol.4 No.5

The aim of this study is to develop a 3D registration algorithm for positron emission tomography/computed tomography (PET/CT) and magnetic resonance (MR) images acquired from independent PET/CT and MR imaging systems. Combined PET/CT images provide anatomic and functional information, and MR images have high resolution for soft tissue. With the registration technique, the strengths of each modality image can be combined to achieve higher performance in diagnosis and radiotherapy planning. The proposed method consists of two stages: normalized mutual information (NMI)-based global matching and independent component analysis (ICA)-based refinement. In global matching, the field of view of the CT and MR images are adjusted to the same size in the preprocessing step. Then, the target image is geometrically transformed, and the similarities between the two images are measured with NMI. The optimization step updates the transformation parameters to efficiently find the best matched parameter set. In the refinement stage, ICA planes from the windowed image slices are extracted and the similarity between the images is measured to determine the transformation parameters of the control points. B-spline. based freeform deformation is performed for the geometric transformation. The results show good agreement between PET/CT and MR images.

The exploration of foreign direct investment market in the E-commerce industry: Evidence from vietnam

KiSung Lee(이기성),Kyungmi Kim(김경미),Hyunjun Park(박현준) 융복합지식학회 2019 융복합지식학회논문지 Vol.7 No.2

이 연구는 베트남의 전자상거래 산업의 신흥 마켓을 조사했다. 과거에는 중국이 전자상거래 산업의 선두주자였으나, 최근 베트남은 풍부한 자원과 값싼 노동력으로 전자상거래에 대한 많은 관심을 표명해왔다. 또한, 베트남은 글로벌 기업의 진출에 대한 상업 시장 조성에 적극 나서고 있어서 전자상거래 시장이 빠르게 활성화 되고 있다. 한국 기업들은 중국시장의 법인세율 인상과 인건비 상승으로 베트남 전자상거래 시장에 진출하는데 높은 관심을 갖고 있다. 따라서, 본 연구는 베트남 전자상거래 시장에 대한 외국의 직접 투자와 전략적 자산모색에 대해 탐구하였다. 이 연구는 전자상거래 시장에서 한국 기업이 베트남에 성공적으로 진출할 수 있는 방법을 베트남 전자상거래 시장의 빠른성장과 현금 유통비율의 감소, 전자결제망 구축, 급속 온라인 모바일 쇼핑 시장을 증가원인에서 찾고자 한다. This research investigated the emerging market of e-commerce industry in Vietnam. Predominately, China created and have sustained a high rate of interest and performance in this area. However, recently, Vietnam has been a promising market because of its abundant resources and cheap labor force. Furthermore, the government is actively creating a commercial market for global companies to enter their country. South Korean companies have a high interest in entering the Vietnamese e-commerce market than China because of raising corporate tax rates and rising labor costs. Thus, this research explored the foreign direct investment in the market and strategic seeking of the e-commerce market in Vietnam. This paper discovered the fast growth of the Vietnamese e-commerce market because the Vietnamese government decided to reduce cash circulation and increase electronic payment, electronic payment network establishment, rapid online mobile shopping market. Also, it analyzed how South Korean companies can enter Vietnam in the e-commerce market successfully.