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      • KCI등재

        Effect of Nitrogen-Load Condition on Hydrogen Production and Bacterial Community in Continuous Anaerobic Hydrogen Fermentation Process

        Kawagoshi, Yasunori,Nakao, Masaharu,Hino, Naoe,Iwasa, Tomonori,Furukawa, Kenji Korean Wetlands Society 2007 한국습지학회지 Vol.9 No.1

        Effect of nitrogen-load condition on hydrogen ($H_2$) production and bacterial community in a continuous anaerobic hydrogen fermentation were investigated. The slight $H_2$ production on extremely low nitrogen-load condition (C/N ratio: 180) at the start-up period. The highest $H_2$ production was obtained when the C/N ratio was 36, the $H_2$ production yield ($mol-H_2/mol-glucose$) reached to 1.7, and it was indicated that Clostridium pasteurianum mainly contributed to the $H_2$ production. The $H_2$ production was decreased on both the lower (C/N: 72) and higher (C/N: 18) nitrogen-load conditions. The excess nitrogen-load was not always suitable for the hydrogen production. The fluctuation of $H_2$ production seemed to be caused by a change in the bacterial community according to the nitrogen-load condition, while a recovery of $H_2$ productivity was possible by a control of nitrogen-load condition through the bacterial community change. When the nitrogen-load condition was not suitable for hydrogen production, the lactic acid concentration was increased and also lactic acid bacteria were definitely detected, which suggested that the competition between hydrogen fermentator and lactic acid producer was occurred. These results demonstrated that the nitrogen-load condition affect on the $H_2$ productivity through the change of bacterial community in anaerobic hydrogen fermentation.

      • KCI등재후보

        Effect of Nitrogen-Load Condition on Hydrogen Production and Bacterial Community in Continuous Anaerobic Hydrogen Fermentation Process

        Yasunori Kawagoshi,Masaharu Nakao,Naoe Hino,Tomonori Iwasa,Kenji Furukawa 한국습지학회 2007 한국습지학회지 Vol.9 No.1

        Effect of nitrogen-load condition on hydrogen (H2) production and bacterial community in a continuous anaerobic hydrogen fermentation were investigated. The slight H2 production on extremely low nitrogen-load condition (C/N ratio: 180) at the start-up period. The highest H2 production was obtained when the C/N ratio was 36, the H2 production yield (mol-H2 / mol-glucose) reached to 1.7, and it was indicated that Clostridium pasteurianum mainly contributed to the H2 production. The H2 production was decreased on both the lower (C/N: 72) and higher (C/N: 18) nitrogen-load conditions. The excess nitrogen-load was not always suitable for the hydrogen production. The fluctuation of H2 production seemed to be caused by a change in the bacterial community according to the nitrogen-load condition, while a recovery of H2 productivity was possible by a control of nitrogen-load condition through the bacterial community change. When the nitrogen-load condition was not suitable for hydrogen production, the lactic acid concentration was increased and also lactic acid bacteria were definitely detected, which suggested that the competition between hydrogen fermentator and lactic acid producer was occurred. These results demonstrated that the nitrogen-load condition affect on the H2 productivity through the change of bacterial community in anaerobic hydrogen fermentation.

      • KCI등재

        Stable Fermentative Hydrogen Production by Polyvinyl Alcohol (Pva) Gel Beads Fluidized Bed Reactor

        Nakao, Masaharu,Kawagoshi, Yasunori,Hino, Naoe,Iwasa, Tomonori,Furukawa, Kenji Korean Wetlands Society 2007 한국습지학회지 Vol.9 No.1

        A novel hydrogen fermentation technique by using polyvinyl alcohol (PVA) gel beads as a biomass carrier was investigated. The hydrogen gas was stably produced throughout the experimental period in a continuous reactor. Even though the hydrogen productivity was suddenly decrease by experimental troubles, the bacteria attached to the PVA gel beads played as an inoculum, it was promptly recovered. The hydrogen yield per glucose was not very high ($1.0-1.2mol-H_2/mol-glucose$), thus the optimization of the experimental conditions such as ORP and HRT should be considered to improve the hydrogen productivity. Bacterial community was stable during experimental period after the PVA gel beads applying, which indicated that applying of biomass carrier was specific to keep not only the biomass but also the bacteria commonly. Clostridium species were phylogenetically detected, which suggested that these bacteria contributed to the hydrogen production in the biofilm attached to the PVA gel beads.

      • KCI등재후보

        Stable Fermentative Hydrogen Production by Polyvinyl Alcohol (Pva) Gel Beads Fluidized Bed Reactor

        Masaharu Nakao,Yasunori Kawagoshi,Naoe Hino,Tomonori Iwasa,Kenji Furukawa 한국습지학회 2007 한국습지학회지 Vol.9 No.1

        A novel hydrogen fermentation technique by using polyvinyl alcohol (PVA) gel beads as a biomass carrier was investigated. The hydrogen gas was stably produced throughout the experimental period in a continuous reactor. Even though the hydrogen productivity was suddenly decrease by experimental troubles, the bacteria attached to the PVA gel beads played as an inoculum, it was promptly recovered. The hydrogen yield per glucose was not very high (1.0 – 1.2mol-H2/mol-glucose), thus the optimization of the experimental conditions such as ORP and HRT should be considered to improve the hydrogen productivity. Bacterial community was stable during experimental period after the PVA gel beads applying, which indicated that applying of biomass carrier was specific to keep not only the biomass but also the bacteria commonly. Clostridium species were phylogenetically detected, which suggested that these bacteria contributed to the hydrogen production in the biofilm attached to the PVA gel beads..

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