RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Transcriptome deep sequencing, identification of novel microRNAs and validation under drought stress in turmeric (Curcuma longa L.)

        R. Santhi,T. E. Sheeja,K. S. Krishnamurthy 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.4

        microRNAs (miRNAs) are small non-coding RNAs that execute regulation of gene expression at the post-transcriptional level. Information on turmeric miRNAs is very rare. In the present study, we have identified 27 conserved and 40 non-conserved miRNA families by high-throughput Illumina sequencing and downstream in silico analysis. We could also identify 97 novel candidate miRNAs specific to turmeric. Selected miRNAs were validated by stem-loop RT-PCR and northern blotting. The targets were predicted for identified miRNAs and were found to be involved in plant growth and development, stress response and metabolism. Expression pattern of selected conserved miRNAs in response to drought stress was analyzed by stem-loop qRT-PCR. This is the first report on miRNA content of turmeric transcriptome, which might serve as a foundation for more future work on miRNA based gene regulation in this medicinally important spice crop.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼