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Jung, In Hye,Chung, Yong-Yoon,Jung, Dawoon E.,Kim, Young Jin,Kim, Do Hee,Kim, Kyung-Sik,Park, Seung Woo Neoplasia Press 2016 Neoplasia Vol.18 No.8
<P>Severe combined immunodeficiency (SCID) mice have widely been used as hosts for human tumor cell xenograft study. This animal model, however, is labor intensive. As zebrafish is largely emerging as a promising model system for studying human diseases including cancer, developing efficient immunocompromised strains for tumor xenograft study are also demanded in zebrafish. Here, we have created the <I>Prkdc</I>-null SCID zebrafish model which provides the stable immune-deficient background required for xenotransplantation of tumor cell. In this study, the two transcription activator-like effector nucleases that specifically target the exon3 of the zebrafish <I>Prkdc</I> gene were used to induce a frame shift mutation, causing a complete knockout of the gene function. The SCID zebrafish showed susceptibility to spontaneous infection, a well-known phenotype found in the SCID mutation. Further characterization revealed that the SCID zebrafish contained no functional T and B lymphocytes which reflected the phenotypes identified in the mice SCID model. Intraperitoneal injection of human cancer cells into the adult SCID zebrafish clearly showed tumor cell growth forming into a solid mass. Our present data show the suitability of using the SCID zebrafish strain for xenotransplantation experiments, and <I>in vivo</I> monitoring of the tumor cell growth in the zebrafish demonstrates use of the animal model as a new platform of tumor xenograft study.</P>
Dynamic 3-D Visualization of the Korea Pathfinder Lunar Orbiter Attitude Control Simulator
Dawoon Jung,Jae Wook Kwon,Kwangyul Baek,Han Woong Ahn,Jong-Wook Choi 제어로봇시스템학회 2020 제어로봇시스템학회 국제학술대회 논문집 Vol.2020 No.10
Visualizing the Korea Pathfinder Lunar Orbiter (KPLO) attitude control behavior is useful for verifying dynamical characteristics unseen in previous Earth-orbiting satellite missions. This paper describes a visualization component of the LUNASIM simulator running unmodified attitude control flight software that is able to communicate with an external program to animate realistically in three dimensions the simulated attitude and orbital state, thruster firings, and solar array rotations of KPLO at faster-than real-time speeds for intuitive design verification. Visualization is also supported when LUNASIM is connected to a processor emulator in a fully end-to-end simulated ETB environment. Results are presented illustrating accurate spacecraft dynamics during special mission scenarios such as roll maneuvering while Sun-pointing near the ecliptic plane, yaw-flipped lunar orbit attitude, and slewing to image the lunar surface while in 45-degree tilted attitude. These results demonstrate the value of visualizing attitude control simulator output for novel spacecraft missions.
JUNG, IN HYE,KIM, DO HEE,YOO, DA KYUNG,BAEK, SUN YOUNG,JEONG, SEONG HOON,JUNG, DAWOON E.,PARK, SEUNG WOO,CHUNG, YONG-YOON ANTICANCER RESEARCH 2018 IN VIVO -ATHENS- Vol.32 No.4
<P>Background/Aim: Natural killer (NK) cells are one of the lymphocytes clinically used for various cancer types. Cytotoxicity of NK cells to cholangiocarcinoma (CC), however, has not yet been studied. Nor NK cell therapy against CC has been clinically applied. In this study, relevance of NK cell therapy for anti-tumor efficacy against CC was pre-clinically investigated. Materials and Methods: Human HuCCT-1 cells, an intrahepatic CC cell line, were xenografted into nude mice. The HuCCT-1 tumor-bearing nude mice then received multiple infusions of ex vivo-expanded human NK cells (SMT01) and in vivo cytotoxic activity of the NK cells against the CC cells was evaluated. Results: SMT01 infusion resulted in significant inhibition of the CC tumor growth. Body weight of the mice administrated with chemotherapy was found to be maintained at the lowest level among all treatment groups while all the SMT01 infusion groups well maintained their body weight. Conclusion: The present in vivo study demonstrates that NK cells contain cytolytic activity against cholangiocarcinoma and show beneficial effect of NK cell therapy in relevance to quality of life. Further investigation of the NK cell-based immunotherapy can be useful to determine cancer therapeutics for the specific tumor.</P>
환경시료에 존재하는 미생물 배양을 위한 filter plate micro trap의 개발
Dawoon Jung(정다운),Tae-Seok Ahn(안태석) 한국생명과학회 2012 생명과학회지 Vol.22 No.6
난배양성 미생물의 배양과 보다 다양한 미생물의 분리를 위하여 Filter plate microbial trap (FPMT)을 개발하였고, 이 장치를 사용하여 Greenland의 토양에서 세균을 분리하였다. FPMT는 배지와 하단부의 membrane filter(0.45 ㎛ pore size)로 구성되어 있다. 시료에 포함된 세균과 화학물질이 FPMT 내로 이동하고, 세균이 증식하는 원리이다. 그 결과, FPMT를 이용한 새로운 in situ 배양방법을 통해 통상적인 Petri dish 배양방법보다 더 많은 종과 기존의 방법으로는 배양할 수 없는 세균을 분리하였다. 이 장치는 앞으로 다양한 환경에서 난배양성 미생물을 분리하는데 유용함을 확인하였다. Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45 ㎛ pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.