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- 저자 : Juanjuan Qu (검색결과 5 건)
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Biosorption mechanism of Zn from aqueous solution by spent substrates of pleurotus ostreatus
XiaoJing Hu,JuanJuan Qu,LiLong Yan,Haidong Gu,TingTing Zang,Yu Jin 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11
To solve the problem of heavy metal pollution and agricultural wastes reclamation, spent substrate of pleurotusostreatus (SSPO) was used as adsorbent to remove Zn2+from aqueous solution. The biosorption of zinc ions on SSPOwas studied as a function of the solution pH, temperature and initial Zn2+concentration. The equilibrium sorption datawere well represented by linear Langmuir isotherm models with R2value of 0.9955 and non-linear Freundlich withR2value of 0.9973. The BET surface area of SSPO can reach 51.16 m2g−1. SEM-EDX and XRD revealed that (NH4)2Zn·H2O and Zn2PO4(OH) were the main compounds in metal-loaded SSPO. FTIR analysis indicated the governing func-tional groups such as O-H, N-H and P=O played an important role in biosorption. The desorption studies showed thereversibility of SSPO. The results indicate that SSPO is a potential adsorbent in wastewater treatment due to its greatsorption capacity and low cost.
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( Sumei Yu ),( Chunying Teng ),( Xin Bai ),( Jinsong Liang ),( Tao Song ),( Liying Dong ),( Yu Jin ),( Juanjuan Qu ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8
In this study, the siderophore-producing characteristics and conditions of Bacillus sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in Brassica juncea were determined. Results of single factor experiment showed that glucose, pH, and Pb(NO<sub>3</sub>)<sub>2</sub> could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at the glucose concentration of 21.84 g/l, pH 6.18, and Pb(NO<sub>3</sub>)<sub>2</sub> concentration of 245.04 μmol/l. The type of siderophore was hydroxamate and its concentration in the fermentation broth amounted to 32.24 μg/ml. Results of pot experiments indicated that the siderophores enhanced B. juncea to assimilate more Pb from soil with the uptake ratio from 1.04 to 2.74, and to translocate more Pb from underground to overground with the TF values from 1.21 to 1.48. The results revealed that Bacillus sp. PZ-1 could produce abundant siderophores and might be potentially used to augment the phytoextraction of Pb from soil.
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Sumei Yu,Chunying Teng,Jinsong Liang,Tao Song,Liying Dong,Xin Bai,Yu Jin,JuanJuan Qu 한국미생물학회 2017 The journal of microbiology Vol.55 No.11
In this study, an antagonistic bacterium against Fusarium oxysporum was identified and designated as Pseudomonas syringae strain BAF.1 on the basis of 16S rDNA sequence analysis and physiological-biochemical characteristics. It produced catechol-species siderophore at a molecular weight of 488.59 Da and a maximum amount of 55.27 μg/ml with glucose as a carbon source and asparagine as a nitrogen source at a C/N ratio of 10:1, 30°C and pH 7. The siderophore exhibited prominent antagonistic activity against Fusarium oxysporum with a maximum inhibition rate of 95.24% and had also suppressive effects on other kinds of 11 phytopathogenic fungi in the absence of FeCl3·6H2O. Spore germination was completely inhibited by 50 μl of the siderophorecontaining solution, and the ultrastructures of mycelia and spores were also considerably suppressed by siderophore treatment as established by electron microscopy observation. These results indicate that the siderophore produced by Pseudomonas syringae BAF.1 could be potentially used for biocontrol of pathogenic Fusarium oxysporum.
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Yang Hu,Shanyi Shi,Lu Lu,Chunying Teng,Sumei Yu,Xin Wang,Min Yu,Jinsong Liang,Juanjuan Qu 한국고분자학회 2017 Macromolecular Research Vol.25 No.3
Excessive reactive oxygen species is implicated in the etiology and pathology of many acute and chronic diseases. Selenium acts as an antioxidant and stimulates the creation of more antioxidants. Selenizing modification polysaccharides potentiate the physiological and pharmacological activities of selenium and polysaccharide. In this study, Inonotus obliquus polysaccharide (IOP) extracted from cultured mycelia was modified into Se-IOP by HNO3- Na2SeO3 method. The characteristics and antioxidant activity of IOP and Se-IOP were comparatively investigated. The result showed that polysaccharide content of purified IOP was 98.9%. The selenium content of Se-IOP was 0.71 mg/g. Both IOP and Se-IOP were homogeneous polysaccharides with a molecular weight of 37.354 and 28.071 kDa, respectively depicted in high performance gel permeation chromatogram (HPGPC). Moreover, both IOP and Se- IOP were composed of Man, Glu, and Gal with a molar ratio of 7.7:32.6:23.3 and 8.3:32.1:22.7, respectively determined by high performance liquid chromatography (HPLC). The characteristic absorption peak of O-Se-O, Se=O and C-O-Se appeared at 1026.75, 772.82, and 652.29 cm-1 respectively in FT-IR spectrum of Se-IOP indicating the success of selenylation. NMR spectrum further confirmed the anomeric carbon signals and chemical shifts in IOP and Se-IOP. These variations induced by selenylation may lead to a higher antioxidant activity of Se-IOP on scavenging hydroxyl, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and superoxide radical than that of IOP. Furthermore, Se- IOP treatment could also reduce oxidant damage by decreasing the level of malondialdehyde (MDA) and increasing the activities of superoxidedismutase (SOD) and GSH-Px in mice.
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Biosorption of Lead(II) by Arthrobacter sp. 25: Process Optimization and Mechanism
( Yu Jin ),( Xin Wang ),( Tingting Zang ),( Yang Hu ),( Xiaojing Hu ),( Guangming Ren ),( Xiuhong Xu ),( Juanjuan Qu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.8
In the present work, Arthrobacter sp. 25, a lead-tolerant bacterium, was assayed to remove lead(II) from aqueous solution. The biosorption process was optimized by response surface methodology (RSM) based on the Box-Behnken design. The relationships between dependent and independent variables were quantitatively determined by second-order polynomial equation and 3D response surface plots. The biosorption mechanism was explored by characterization of the biosorbent before and after biosorption using atomic force microscopy (AFM), scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The results showed that the maximum adsorption capacity of 9.6 mg/g was obtained at the initial lead ion concentration of 108.79 mg/l, pH value of 5.75, and biosorbent dosage of 9.9 g/l (fresh weight), which was close to the theoretically expected value of 9.88 mg/g. Arthrobacter sp. 25 is an ellipsoidalshaped bacterium covered with extracellular polymeric substances. The biosorption mechanism involved physical adsorption and microprecipitation as well as ion exchange, and functional groups such as phosphoryl, hydroxyl, amino, amide, carbonyl, and phosphate groups played vital roles in adsorption. The results indicate that Arthrobacter sp. 25 may be potentially used as a biosorbent for low-concentration lead(II) removal from wastewater.
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