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RISS 인기검색어
- 검색키워드
- 저자 : Joonhee Lee (검색결과 168 건)
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Current status and management of fluvalinate resistance of Varroa mites in Korea
Susie Cho,Joonhee Lee,Youngcheon Lim,Jonghyeok Lee1,Si Hyeock Lee 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
The Varroa mite, Varroa destructor is an ectoparasite that parasitizes honey bees. The widespread usage of acaricides, particularly fluvalinate, has resulted in the emergence of resistance in Varroa mite populations all over the world. The goal of this study is to track fluvalinate resistance in Varroa mite field populations in Korea using both bioassay and molecular markers. To accomplish this, a residual contact vial (RCV) bioassay for on-site resistance monitoring was developed. Early mortality evaluation in the RCV bioassay was effective for reliably separating mites with the knockdown resistance (kdr) genotype, but late mortality evaluation was useful for distinguishing mites with additional resistance factors. The RCV bioassay of 14 field mite populations collected in 2021 revealed potential resistance development in four populations. Quantitative sequencing was used as an alternate method to examine the frequency of the L925I/M mutation in the voltage-gated sodium channel (vgsc), which is related with the fluvalinate kdr phenotype. While the mutation was not present in Varroa mite populations in 2020, it appeared in 2021, rose in frequency in 2022, and was practically ubiquitous across the country by 2023. This recent emergence and rapid spread of fluvalinate resistance within a span of three years demonstrate the Varroa mite's significant potential for developing resistance. This situation emphasizes the critical necessity to replace fluvalinate with alternate acaricides, such as fenpyroximate, coumaphos, and amitraz. A few novel vgsc mutations potentially involved in resistance were identified. Potential factors driving the rapid expansion of resistance were further discussed.
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Development of an infant formula certified reference material for the analysis of organic nutrients
Lee, Joonhee,Kim, Byungjoo,Lee, Sun Young,Choi, Jongoh,Kang, Dukjin,Lee, Hwasim,Choi, KiHwan,Lee, Hyeyoung,Sim, Hee-Jung,Baek, Song-Yee,Lee, Honghee,Hyung, Seok-Won,Ahn, Seonghee,Seo, Dongwon,Hwang, J Applied Science Publishers 2019 Food chemistry Vol.298 No.-
<P><B>Abstract</B></P> <P>Infant formula certified reference material (CRM, KRISS CRM 108-02-003) were developed for the analysis of organic nutrients. The CRM is a milk-based infant formula powder, packaged at 14 g per unit. Ten thousand units were prepared and stored at −70 °C. For the certification of each nutrient, ten units were analyzed for simultaneous value-assignment and homogeneity test. Analytical methods used were isotope dilution mass spectrometry (IDMS) based on liquid chromatography mass spectrometer (LC/MS) or gas chromatography mass spectrometer (GC/MS) as higher-order reference methods.13 vitamins, 3 fatty acids, and total cholesterol were certified. The between-unit relative standard deviation of measurement results for each nutrient ranged 0.2% to 2.5%, showing very good homogeneity. The expanded relative uncertainties of the certified values ranged from 1% to 8%, indicating that they have higher-order metrological quality. The values of proximates (proteins, lipids, carbohydrates, water, and ash) were assigned through inter-laboratory comparisons.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An infant formula CRMs for the analysis of organic nutrients was developed. </LI> <LI> Organic nutrients were certified by IDMS approaches as higher-order reference methods. </LI> <LI> Homogeneities and stability of the CRM were evaluated by IDMS approaches. </LI> <LI> Metrological qualities of the certified values were proved by their small uncertainties. </LI> <LI> Five proximates were value-assigned by interlaboratory comparison. </LI> </UL> </P>
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Polarization-dependent GaN surface grating reflector for short wavelength applications
Lee, Joonhee,Ahn, Sungmo,Chang, Hojun,Kim, Jaehoon,Park, Yeonsang,Jeon, Heonsu The Optical Society 2009 Optics express Vol.17 No.25
<P>This study proposes a one-dimensional sub-wavelength grating structure on GaN surface which behaves as a reflector for transverse-electric polarized light in the blue wavelength range. The rigorous coupled-wave analysis method was used to analyze the effects of various structural parameters on the reflectance spectra of the grating. Based on the optimal design, a GaN surface grating reflector (SGR) was fabricated using holographic lithography and dry etching processes. It showed reflectance that exceeded 90% over a 60-nm bandwidth. The obtained experimental results were in good agreement with simulated ones. The SGR has an advantage of structural simplicity, which should greatly facilitate the fabrication and integration of high reflectors on GaN-based short-wavelength photonic devices.</P>
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Lee, Joonhee,Kim, Byungjoo Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.8 No.3
Ibuprofen is one of the most popular analgesic and antipyretic drugs. An isotope dilution mass spectrometry method based on LC/MS was developed as a candidate reference method for the accurate determination of ibuprofen in pharmaceutical tablets. Isotope labelled ibuprofen, $ibuprofen-d_3$, was added as an internal standard into sample extracts. Ibuprofen and $ibuprofen-d_3$, was analysed by LC/MS in a selected ion monitoring (SIM) mode to detect ions at m/z 205 and 208, respectively. The repeatability and reproducibility of the developed ID-LC/MS method were tested for the validation and assessment of metrological quality of the method.
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( Joonhee Lee ),( Byungjoo Kim ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.3
Ibuprofen is one of the most popular analgesic and antipyretic drugs. An isotope dilution mass spectrometry method based on LC/MS was developed as a candidate reference method for the accurate determination of ibuprofen in pharmaceutical tablets. Isotope labelled ibuprofen, ibuprofen-d3, was added as an internal standard into sample extracts. Ibuprofen and ibuprofen- d3, was analysed by LC/MS in a selected ion monitoring (SIM) mode to detect ions at m/z 205 and 208, respectively. The repeatability and reproducibility of the developed ID-LC/MS method were tested for the validation and assessment of metrological quality of the method.
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External Mergesort for Flash-Based Solid State Drives
Joonhee Lee,Hongchan Roh,Sanghyun Park IEEE 2016 IEEE Transactions on Computers Vol. No.
<P>Mergesort is the most widely-known external sorting algorithm, which is used when the data being sorted do not fit into the available main memory. There have been several attempts to improve mergesort by reducing I/O time, since mergesort is I/O intensive. However, these methods assumed that mergesort runs on hard disk drives (HDDs). Flash-based solid state drives (SSDs) are emerging as next generation storage devices and becoming alternatives to HDDs. SSDs outperform HDDs in access latency, because they have no physical arms to move. In addition, SSDs benefit from their inner structure by exploiting internal parallelism, resulting in high I/O bandwidth. Previous methods for improving mergesort focused on reducing random access cost, which is insignificant on SSDs. In this paper we propose an external mergesort algorithm for SSDs called FMsort. FMsort calculates a block read order which is the order of blocks needed in the merge phase. With a block read order, a number of blocks required during the merge phase are read into main memory via multiple asynchronous I/Os. Our experiments show that FMsort outperforms other mergesort algorithms, at an invisible cost of calculating a block read order.</P>
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Lee, Hyeyoung,Lee, Joonhee,Choi, Kihwan,Kim, Byungjoo Applied Science Publishers 2017 Food chemistry Vol.221 No.-
<P><B>Abstract</B></P> <P>A method based on isotope dilution-liquid chromatography/tandem mass spectrometry (ID-LC/MS/MS) using a C30 column has been developed for the separate and accurate determination of <I>trans</I>- and <I>cis</I>-vitamin K<SUB>1</SUB> in infant formula. Vitamin K<SUB>1</SUB> and the deuterium-labeled internal standard eluted at slightly different retention times experiencing different matrix effects, and this possibly resulted in biased measurement. The matrix effect profiles obtained from post-column infusion experiments showed that atmospheric pressure chemical ionization (APCI) was less susceptible to matrix effects near the retention time than electrospray ionization (ESI); therefore, APCI was used in this study. The developed method was validated by measuring fortified samples, and the results agreed with the gravimetric values. Its repeatability and reproducibly were within 2% relative standard deviation. The relative expanded uncertainty was approximately 5%, indicating that the method was of higher-order metrological quality as a reference method.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Application of ID-LC/MS/MS for the accurate determination of vitamin K<SUB>1</SUB> isomers. </LI> <LI> Quantification of <I>trans</I>- and <I>cis</I>-vitamin K<SUB>1</SUB> in the primary reference material by NMR. </LI> <LI> Minimization of the measurement bias using APCI rather than ESI. </LI> <LI> Developed method was proven to have high-order metrological quality. </LI> </UL> </P>
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Lee, Jaeick,Lee, Seunghwa,Kim, Byungjoo,Lee, Joonhee,Kwon, Oh-Seung,Cha, Eunju Korean Society for Mass Spectrometry 2018 Mass spectrometry letters Vol.9 No.1
A quantitation method for free amino acids in human serum was developed using a stepwise-dilution method and a bimodal cation exchange (CEX)/hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry system equipped with an electrospray ionization source (ESI/MS/MS). This method, which was validated using quality control samples, was optimized for enhanced selectivity and sensitivity. Dithiothreitol (DTT) was used as a reducing agent to prevent the oxidation of a serum sample ($50{\mu}L$), which was then subjected to stepwise dilution using 3, 30, and 90 volumes of acetonitrile containing 0.1% formic acid. Chromatographic separation was performed on an Imtakt Intrada Amino Acid column ($50mm{\times}3mm$, $3{\mu}m$) in mixed mode packed with CEX and HILIC ligands embedded in the stationary phase. Underivatized free amino acids were eluted and separated within 10 min. As a result of the validation, the precision and accuracy for the inter- and intraday assays were determined as 2.11-11.51% and 92.82-109.40%, respectively. The lowest limit of quantification (LLOQ) was $0.5-4.0{\mu}g/mL$ and the matrix effect was 80.22-115.93%. The proposed method was successfully applied to the quantitative analysis of free amino acids in human serum.
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