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- 저자 : Jingsong He (검색결과 2 건)
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Jingsong He,Ni Xie,Jianbo Yang,Hong Guan,Weicai Chen,Huisheng Wu,Zishan Yuan,Kun Wang,Guojin Li,Jie Sun,Limin Yu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.3
Purpose: Synuclein-γ (SNCG), which was initially identified asbreast cancer specific gene 1, is highly expressed in advancedbreast cancers, but not in normal or benign breast tissue. Thisstudy aimed to evaluate the effects of SNCG siRNA-treatmenton breast cancer cells and elucidate the associated mechanisms. Methods: Vectors containing SNCG and negative control(NC) siRNAs were transfected into MDA-MB-231 cells; mRNAlevels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migrationwas assessed by the Transwell assay, apoptosis and cellcycle analyses were conducted with the flow cytometer, andWestern blot analysis was performed to determine the relativelevels of AKT, ERK, p-AKT, and p-ERK expression. Results:SNCG mRNA levels were significantly reduced in MDA-MB-231cells transfected with SNCG siRNA. Our results indicate that inSNCG siRNA-treated cells, cell migration and proliferation decreasedsignificantly, apoptosis was induced, and the cell cyclewas arrested. Western blot analysis indicated that the proteinlevels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups. Conclusion:SNCG siRNA could decrease the migration and proliferationof breast cancer cells by downregulating the phosphorylationof AKT and ERK.
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Exploring precise deposition and influence mechanism for micro-scale serpentine structure fiber
Wang, Han,Ou, Weicheng,Zhong, Huiyu,He, Jingfan,Wang, Zuyong,Cai, Nian,Chen, XinDu,Xue, Zengxi,Liao, Jianxiang,Zhan, Daohua,Yao, Jingsong,Wu, Peixuan Techno-Press 2022 Advances in nano research Vol.12 No.2
Micro-scale serpentine structure fibers are widely used as flexible sensor in the manufacturing of micro-nano flexible electronic devices because of their outstanding non-linear mechanical properties and organizational flexibility. The use of melt electrowriting (MEW) technology, combined with the axial bending effect of the Taylor cone jet in the process, can achieve the micro-scale serpentine structure fibers. Due to the interference of the process parameters, it is still challenging to achieve the precise deposition of micro-scale and high-consistency serpentine structure fibers. In this paper, the micro-scale serpentine structure fiber is produced by MEW combined with axial bending effect. Based on the controlled deposition of MEW, applied voltage, collector speed, nozzle height and nozzle diameter are adjusted to achieve the precise deposition of micro-scale serpentine structure fibers with different morphologies in a single motion dimension. Finally, the influence mechanism of the above four parameters on the precise deposition of micro-scale serpentine fibers is explored.
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