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- 저자 : Jianfeng Shan (검색결과 3 건)
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Shan Jianfeng,Liang Yuanxiao,Yang Zhili,Chen Wenshan,Chen Yun,Sun Ke 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.3
This study aims to explore possible effect of RNA polymerase I subunit D (POLR1D) on proliferation and angiogenesis ability of colorectal cancer (CRC) cells and mechanism herein. The correlation of POLR1D and Yin Yang 1 (YY1) expressions with prognosis of CRC patients in TCGA database was analyzed. Quantitative realtime polymerase chain reaction (qRT-PCR) and Western blot were applied to detect expression levels of POLR1D and YY1 in CRC cell lines and CRC tissues. SW480 and HT- 29 cells were transfected with si-POLR1D or pcDNA3.1-POLR1D to achieve POLR1D suppression or overexpression before cell migration, angiogenesis of human umbilical vein endothelial cells were assessed. Western blot was used to detect expressions of p38 MAPK signal pathway related proteins and interaction of YY1 with POLR1D was confirmed by dual luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). TCGA data showed that both POLR1D and YY1 expressions were up-regulated in CRC patients. High expression of POLR1D was associated with poor prognosis of CRC patients. The results showed that POLR1D and YY1 were highly expressed in CRC cell lines. Inhibition or overexpression of POLR1D can respectively suppress or enhance proliferation and angiogenesis of CRC cells. YY1 inhibition can suppress CRC progression and deactivate p38 MAPK signal pathway, which can be counteracted by POLR1D overexpression. JASPAR predicted YY1 can bind with POLR1D promoter, which was confirmed by dual luciferase reporter gene assay and ChIP. YY1 transcription can up-regulate POLR1D expression to activate p38 MAPK signal pathway, thus promoting proliferation and angiogenesis ability of CRC cells
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Honggui Wan,Jianfeng Yuan,Xianyang Shan,Qiyang Wu,Nan Shi 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4
A gray and low viscosity extracellular polysaccharide (EPS) composed of N-acetylglucosamine, xylose,and mannose was isolated from culture medium of Bacillus sp. strain LBP32 by ethanol precipitation followed by dialysis and freeze-drying. The crude biopolymer showed an apparent molecular weight (Mw) of ~ 9.62 × 10^4. Chemical and spectroscopic studies revealed that the bacterial biopolymer was composed of a β-1,4-linked backbone carrying a low content of β-1,3-linked backbone. In addition, the EPS demonstrated a high antioxidant activity in a concentration dependent manner. The 50%inhibition concentration (IC_(50)) for quenching hydroxyl radical (·OH) and superoxide radical (·O_2^−) were 0.042 and 0.165 mg/mL, respectively. Furthermore, the EPS demonstrated a strong protective effect against lipid peroxidation and radiation such as UV radiation and ion beam irradiation. These results indicate that the protective effects of the EPS were most likely due to its free radical scavenging ability.
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Zheng Chen,Xueying Zhao,Shuwei Wei,Dengfeng Wang,Xuelan Zhang,Jianfeng Shan 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.11
In the industrial production, water exists inevitably into feed stocks in the form of impurity, and it can produce a negative effect in the hydrogenation reaction due to the preferential adsorption of water on active sites. Here, the surfactants (polyvinylpyrrolidone, poloxamer, polyethylene glycol and hexadecyl trimethyl ammonium bromide) are used to improve physicochemical property of Cu/SiO2 catalysts, so that Cu/SiO2 catalysts had a good hydrogenation performance at condition containing water. The appropriate addition amount of surfactants in the catalyst preparation process effectively hindered the agglomeration of copper species by steric configuration and repulsion effect between Cu2+ and positive ionizable, which brought about high copper dispersion and small particle size. Meanwhile, the decomposition of surfactants produced many pores during calcination, resulting in the increased of specific surface area and average pore diameter. These advantages provided more chances for reactants to touch active sites due to spatial restriction and the increase of the number of active sites, so that the negative effects of water can be counteracted. The conversion of Cu/SiO2 catalysts, that the surfactants was added in the catalyst preparation process, increased 60% to 200% at reaction condition containing water.
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