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Jeswin, Joseph,Joo, Min-Soo,Jeong, Ji-Min,Bae, Jin-Sol,Choi, Kwang-Min,Cho, Dong-Hee,Park, Son-Il,Park, Chan-Il Elsevier 2018 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.84 No.-
<P><B>Abstract</B></P> <P>Siglec-3/CD33 is a myeloid-specific inhibitory receptor that is expressed on cells of the immune system, where it is believed to play a regulatory role, modulating the inflammatory and immune responses. We characterized CD33 (RbCD33) in rock bream which is a transmembrane protein with two IG-like domains and a cytoplasmic tail. It has a deduced amino acid sequence of 390 residues and has tyrosine-based signaling motifs in the cytoplasmic tail. The RbCD33 mRNA was highly expressed in peripheral blood leukocytes and was also detected in the muscle, spleen, skin, head kidney, gills, trunk kidney, heart, stomach, brain, intestine and liver by quantitative real-time PCR. A temporal variation in expression of RbCD33 was observed in different tissues after stimulating with <I>E. tarda</I>, <I>S. iniae</I> and red seabream iridovirus (RSIV). In the head kidney tissue, <I>E. tarda</I> and <I>S. iniae</I> induced RbCD33, while a down regulation was observed with RSIV. In addition, in spleen tissue, <I>S. iniae</I> caused a very high induction of RbCD33 in comparison with an <I>E. tarda</I> and RSIV challenge. In the liver and gill tissues, all three pathogens induced a high expression of RbCD33. The expression pattern in various tissues and its high induction after pathogen stimulation suggests that RbCD33 plays an important role in initiating the immune response via the inhibition of signal transduction of the myeloid lineage cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Characterized CD33 is a transmembrane protein with two IG like domains. </LI> <LI> Presence of inhibitory motif indicates its regulatory role in innate immunity. </LI> <LI> CD33 highly induced in peripheral blood leukocytes, muscle, spleen and skin. </LI> <LI> <I>E. tarda</I>, <I>S. inia</I> and RSIV stimulation in rock bream induced the expression of CD33. </LI> </UL> </P>
Jeswin, Joseph,Jeong, Seon-Myeong,Jeong, Ji-Min,Bae, Jin-Sol,Kim, Mu-Chan,Kim, Do-Hyung,Park, Chan-Il Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.66 No.-
<P><B>Abstract</B></P> <P>CD28 is a co-stimulatory receptor that provides a critical second signal alongside T cell receptors for the activation of naive T cells. We characterized the CD28 gene of rock bream, which has a deduced amino acid sequence of 221 residues with an extracellular Ig-superfamily V domain, transmembrane region, and cytoplasmic tail. The conservation in domain structures and other motifs shows that it is highly likely that RbCD28 is a homologue of mammalian CD28 and may have related co-stimulatory functions. RbCD28 is constitutively expressed in most tissues that were analysed, with a relatively higher expression in teleost lymphoid organs, such as spleens, gills, trunk kidneys and skin. Unlike human CD28, RbCD28 is highly expressed in skin and gill-associated lymphoid organs. Although gills showed constitutive expression of RbCD28 in control animals, after a pathogen challenge, induction of CD28 was low, particularly in RSIV and <I>E. tarda</I> infection. Whereas induction of RbCD28 was observed in kidney during <I>E. tarda</I> and <I>S. iniae</I> infection, downregulation was observed during RSIV infection. In the case of the liver, <I>E. tarda</I> caused an initial upregulation of RbCD28. RbCD28 activation of T cells in the spleen was limited to <I>S. iniae</I> infection. Activation of RbCD28 observed in lymphoid organs during infection of various pathogens shows its key role as a co-stimulatory receptor of T cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CD28 a T cell costimulatory receptor is identified in Rock bream. </LI> <LI> Conservation in domain and motifs shows that RbCD28 is a homolog of mammalian CD28. </LI> <LI> It was highly expressed in various teleost lymphoid organs including gill and skin. </LI> <LI> <I>E. tarda</I>, <I>S. iniae</I> and RSIV infection caused differential expression of RbCD28 in lymphoid organs. </LI> <LI> Activation of RbCD28 in lymphoid organs during infection shows its key role as a costimulatory receptor of T cells. </LI> </UL> </P>
Hwang, Jee Youn,Jeong, Ji-Min,Kwon, Mun-Gyeong,Seo, Jung Soo,Hwang, Seong Don,Son, Maeng-Hyun,Bae, Jin-Sol,Choi, Kwang-Min,Cho, Dong-Hee,Jeswin, Joseph,Park, Chan-Il Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.73 No.-
<P>Coinhibitory pathways in the B7-CD28 family provide critical inhibitory signals that regulate immune homeostasis, defense and protect tissue integrity. CD276 (B7-H3) is an important immune checkpoint member of this family, which is induced on antigen-presenting cells (APCs), and plays an important role in the inhibition of T cell function. We have characterized the CD276 gene of olive flounder, Paralichthys olivaceus. OfCD276 has an ORF of 912 bp that codes for 303 amino acids with a predicted molecular mass of 33 kDa. It is a type I trans membrane protein with a single extracellular V- and C-like Ig domains, a transmembrane region, and a highly diverse cytoplasmic tail. This gene was distinctly expressed in gill, spleen, and skin, and sparsely expressed in other tissues. Pathogen stimulation by VHSV revealed that transcription of OfCD276 was induced on early hours in liver and expressed late in head kidney, spleen, intestine and gill tissues. Flow cytometry analysis of leukocytes revealed the percentage of granulocytes and lymphocytes that expressed OfCD276 molecules on their cell surface was 85.1% and 3.1%, respectively. Our study shows a significant role played by this coinhibitory molecule that participate in the regulation of the cell mediated immune response.</P>