Association between Mismatch Repair Gene MSH3 codons 1036 and 222 Polymorphisms and Sporadic Prostate Cancer in the Iranian Population

Jafary, Fariba,Salehi, Mansoor,Sedghi, Maryam,Nouri, Nayereh,Jafary, Farzaneh,Sadeghi, Farzaneh,Motamedi, Shima,Talebi, Maede Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

The mismatch repair system (MMR) is a post-replicative DNA repair mechanism whose defects can lead to cancer. The MSH3 protein is an essential component of the system. We postulated that MSH3 gene polymorphisms might therefore be associated with prostate cancer (PC). We studied MSH3 codon 222 and MSH3 codon 1036 polymorphisms in a group of Iranian sporadic PC patients. A total of 60 controls and 18 patients were assessed using the polymerase chain reaction and single strand conformational polymorphism. For comparing the genotype frequencies of patients and controls the chi-square test was applied. The obtained result indicated that there was significantly association between G/A genotype of MSH3 codon 222 and G/G genotype of MSH3 codon 1036 with an increased PC risk (P=0.012 and P=0.02 respectively). Our results demonstrated that MSH3 codon 222 and MSH3 codon 1036 polymorphisms may be risk factors for sporadic prostate cancer in the Iranian population.

Vibration and instability of nanocomposite pipes conveying fluid mixed by nanoparticles resting on viscoelastic foundation

Abolfazl Jafari Natanzi,Gholamreza Soleimani Jafari,Reza Kolahchi 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.21 No.5

In this study, nonlinear vibration and stability of a polymeric pipe reinforced by single-walled carbon naotubes (SWCNTs) conveying fluid-nanoparticles mixture flow is investigated. The Characteristics of the equivalent composite are determined using Mori-Tanaka model considering agglomeration effects. The surrounding elastic medium is simulated by orthotropic visco-Pasternak medium. Employing nonlinear strains-displacements, stress-strain energy method the governing equations were derived using Hamilton's principal. Differential quadrature method (DQM) is used for obtaining the frequency and critical fluid velocity. The influence of volume percent of SWCNTs, agglomeration, geometrical parameters of pipe, viscoelastic foundation and fluid velocity are shown on the frequency and critical fluid velocity of pipe. Results showed the increasing volume percent of SWCNTs leads to higher frequency and critical fluid velocity.

Influence of Nickel-Coated Nanostructured WC-Co Powders on Microstructural and Tribological Properties of HVOF Coatings

Jafari, M.,Enayati, M. H.,Salehi, M.,Nahvi, S. M.,Hosseini, S. N.,Park, C. G. Springer Science + Business Media 2014 Journal of thermal spray technology Vol.23 No.8

Determination of bortezomib and dasatinib anticancer drugs in real samples using an electrochemical sensor modified with new CuFe2O4/SmVO4/ionic liquid nanocomposite

Jafari-Kashi Abbas,Shabani-Nooshabadi Mehdi,Rafiee-Pour Hossain-Ali 한국탄소학회 2024 Carbon Letters Vol.34 No.3

Bortezomib (BTZ) and dasatinib (DA) are two substantial anti-cancer agents with side effects on the human body. In this research, we fabricated a novel electrochemical sensor modified by CuFe2O4/SmVO4 nanocomposite and 1-ethyl-3-methylimidazolium chloride (1E3MC) as an ionic liquid (IL) (CuFe2O4/SmVO4/IL/CPE) for coinciding investigation of BTZ and DA for the first time. The CuFe2O4/SmVO4 synthesized were determined and certified through field-emission scanning electron microscopy (FE-SEM), energy diffraction X-ray (EDX), and X-ray diffraction (XRD). The capability of the sensor was investigated by different electrochemical techniques such as cyclic voltammetry (CV), chronoamperometry (CHA), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The attained data showed that the oxidation signal of bortezomib and dasatinib promoted as an innovative electrochemical sensor. After optimization of the conditions using this sensor at pH 7.0, the oxidation signal of bortezomib and dasatinib showed to be linear with drug concentrations in the range of 0.09–90 µM and 100–500 µM with a detection limit of 5.4 nM and 7.0 µM, respectively, using differential pulse voltammetry method. The values of D and electro-transfer coefficient (α) achieved 2.5 × 10−5 cm2 s−1 and 0.99, respectively. The proposed electrochemical sensor exhibited acceptable selectivity and sensitivity for simultaneous detection of bortezomib and dasatinib in pharmaceutical and biological samples.

Analysis of the power augmentation mechanisms of diffuser shrouded micro turbine with computational fluid dynamics simulations

Jafari, Seyed A.,Kosasih, Buyung Techno-Press 2014 Wind and Structures, An International Journal (WAS Vol.19 No.2

Reported experimental and computational fluid dynamic (CFD) studies have demonstrated significant power augmentation of diffuser shrouded horizontal axis micro wind turbine compared to bare turbine. These studies also found the degree of augmentation is strongly dependent on the shape and geometry of the diffuser such as length and expansion angle. However study flow field over the rotor blades in shrouded turbine has not received much attention. In this paper, CFD simulations of an experimental diffuser shrouded micro wind turbine have been carried out with the aim to understand the mechanisms underpinning the power augmentation phenomenon. The simulations provide insight of the flow field over the blades of bare wind turbine and of shrouded one elucidating the augmentation mechanisms. From the analysis, sub-atmospheric back pressure leading to velocity augmentation at the inlet of diffuser and lowering the static pressure on blade suction sides have been identified as th dominant mechanisms driving the power augmentation. And effective augmentation was achieved for ${\lambda}$ above certain value. For the case turbine it is ${\lambda}$ greater than ${\approx}2$.

Local buckling of thin and moderately thick variable thickness viscoelastic composite plates

Jafari, Nasrin,Azhari, Mojtaba,Heidarpour, Amin Techno-Press 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.6

This paper addresses the finite strip formulations for the stability analysis of viscoelastic composite plates with variable thickness in the transverse direction, which are subjected to in-plane forces. While the finite strip method is fairly well-known in the buckling analysis, hitherto its direct application to the buckling of viscoelastic composite plates with variable thickness has not been investigated. The equations governing the stiffness and the geometry matrices of the composite plate are solved in the time domain using both the higher-order shear deformation theory and the method of effective moduli. These matrices are then assembled so that the global stiffness and geometry matrices of a moderately thick rectangular plate are formed which lead to an eigenvalue problem that is solved to determine the magnitude of critical buckling load for the viscoelastic plate. The accuracy of the proposed model is verified against the results which have been reported elsewhere whilst a comprehensive parametric study is presented to show the effects of viscoelasticity parameters, boundary conditions as well as combined bending and compression loads on the critical buckling load of thin and moderately thick viscoelastic composite plates.

Induction of Apoptosis and Cell Cycle Arrest by Dorema Glabrum Root Extracts in a Gastric Adenocarcinoma (AGS) Cell Line

Jafari, Naser,Zargar, Seyed Jalal,Yassa, Narguess,Delnavazi, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.12

Objective: Dorema glabrum Fisch. & C.A. Mey is a perennial plant that has several curative properties. Anti-proliferative activity of seeds of this plant has been demonstrated in a mouse fibrosarcoma cell line. The aim of the present study was to evaluate cytotoxicity of D. glabrum root extracts in a human gastric adenocarcinoma (AGS) cell line and explore mechanisms of apoptosis induction, cell cycle arrest and altered gene expression in cancer cells. Materials and Methods: The MTT assay was used to evaluate IC50 values, EB/AO staining to analyze the mode of cell death, and flow cytometry to assess the cell cycle. Quantitative real-time polymerase chain reaction (qRT-PCR) amplification was performed with apoptosis and cell cycle-related gene primers, for cyclin D1, c-myc, survivin, VEGF, Bcl-2, Bax, and caspase-3 to determine alteration of gene expression. Results: Our results showed that n-hexane and chloroform extracts had greatest toxic effects on gastric cancer cells with IC50 values of $6.4{\mu}g/ml$ and $4.6{\mu}g/ml$, respectively, after 72 h. Cell cycle analysis revealed that the population of treated cells in the G1 phase was increased in comparison to controls. Cellular morphological changes indicated induction of apoptosis. In addition, mRNA expression levels of Bax and caspase-3 were increased, and of bcl-2 survivin, VEGF, c-myc and cyclin D1 were decreased. Conclusion: Our study results suggest that D. glabrum has cytotoxic effects on AGS cells, characterized by enhanced apoptosis, reduced cell viability and arrest of cell cycling.

Designing an Emotional Intelligent Controller for IPFC to Improve the Transient Stability Based on Energy Function

Jafari, Ehsan,Marjanian, Ali,Solaymani, Soodabeh,Shahgholian, Ghazanfar The Korean Institute of Electrical Engineers 2013 Journal of Electrical Engineering & Technology Vol.8 No.3

The controllability and stability of power systems can be increased by Flexible AC Transmission Devices (FACTs). One of the FACTs devices is Interline Power-Flow Controller (IPFC) by which the voltage stability, dynamic stability and transient stability of power systems can be improved. In the present paper, the convenient operation and control of IPFC for transient stability improvement are considered. Considering that the system's Lyapunov energy function is a relevant tool to study the stability affair. IPFC energy function optimization has been used in order to access the maximum of transient stability margin. In order to control IPFC, a Brain Emotional Learning Based Intelligent Controller (BELBIC) and PI controller have been used. The utilization of the new controller is based on the emotion-processing mechanism in the brain and is essentially an action selection, which is based on sensory inputs and emotional cues. This intelligent control is based on the limbic system of the mammalian brain. Simulation confirms the ability of BELBIC controller compared with conventional PI controller. The designing results have been studied by the simulation of a single-machine system with infinite bus (SMIB) and another standard 9-buses system (Anderson and Fouad, 1977).

GENERALIZED MATRIX FUNCTIONS, IRREDUCIBILITY AND EQUALITY

Jafari, Mohammad Hossein,Madadi, Ali Reza Korean Mathematical Society 2014 대한수학회보 Vol.51 No.6

Let $G{\leq}S_n$ and ${\chi}$ be any nonzero complex valued function on G. We first study the irreducibility of the generalized matrix polynomial $d^G_{\chi}(X)$, where $X=(x_{ij})$ is an n-by-n matrix whose entries are $n^2$ commuting independent indeterminates over $\mathbb{C}$. In particular, we show that if $\mathcal{X}$ is an irreducible character of G, then $d^G_{\chi}(X)$ is an irreducible polynomial, where either $G=S_n$ or $G=A_n$ and $n{\neq}2$. We then give a necessary and sufficient condition for the equality of two generalized matrix functions on the set of the so-called ${\chi}$-singular (${\chi}$-nonsingular) matrices.

The relationship between common mutations in CFTR, AR genes, Y chromosome microdeletions and karyotyping abnormalities with very severe oligozoospermia in Iranian men

Jafari Leyla,Safinejad Kyumars,Nasiri Mahboobeh,Heidari Mansour,Houshmand Massoud 한국유전학회 2023 Genes & Genomics Vol.45 No.4

Background Male infertility due to very severe oligozoospermia has been associated with some genetic risk factors. Objective To investigate the distribution of the mutations in the CFTR gene, the CAG-repeat expansion of the AR gene, also Y chromosome microdeletions and karyotyping abnormalities in very severe oligozoospermia patients. Methods In the present case–control study, 200 patients and 200 fertile males were enrolled. All patients and control group were karyotyped. Microdeletions were evaluated using multiplex PCR. Five common CFTR mutations were genotyped using the ARMS-PCR technique. The CAG-repeat expansion in the AR gene was evaluated for each individual using sequencing. Results Overall 4% of cases shows a numerical and structural abnormality. 7.5% of patients had a deletion in one of the AZF regions on Yq, and 3.5% had a deletion in two regions. F508del was the most common (4.5%) CFTR gene mutation; G542X, and W1282X were detected with 1.5% and 1% respectively. One patient was found to have AZFa microdeletion and F508del in heterozygote form; one patient had AZFb microdeletion with F508del. F508del was seen as compound heterozygous with G542X in one patient and with W1282X in the other patient. The difference in the mean of the CAG-repeats in the AR gene in patients and control groups was statistically significant (P = 0.04). Conclusion Our study shows the genetic mutations in men with severe oligozoospermia and given the possibility of transmission of these disorders to the next generation by fertilization, counseling and genetic testing are suggested for these couples before considering ICSI.