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Jaewon RYU,Si Wouk KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
A novel methanotroph, isolated from the rice paddy field soil, was identified as Methylocystis sp. JR2 by 16srDNA gene sequencing. The strain produced a pink pigment in the NMS medium containing low phosphate concentration (≤0.1mM). Maximal absorption peaks and color of the pigment were changed by the solvents and pHs. Pigment was purified and analyzed by LC-MS/MS with m/z 338.7, 352.6 and 366.7. These values were the typical characteristics of the prodigiosin analogues consisting of 2-methyl-3-hexyl, 2-methyl-3-heptyl and 2-methyl-3-octyl prodiginine, respectively. Maximal productivities of these analogues were 62.9 and 8.1 mg/L for 2-methyl-3-heptyl prodiginine and 2-methyl-3-octyl prodiginine, respectively. To our best knowledge, these were the maximum concentrations obtained from methanotrophs. Whole genome sequencing of the isolate revealed that the genome consisted of a total of five contigs of 4,362,918 bp with GC content of 63.39%. The prodigiosin-related genes were located in contig2 in an operon. The anticancer efficacy of the extracted pigment demonstrated that the IC50 values were 15.6, 500, 250 and 250 ㎍/㎖ in prostate cancer cell line LNCap and PC3, breast cancer cell line MDA-MB-231, and lung cancer cell line NCI H460, respectively. These results showed that the anticancer activity of the pigment was similar to the typical prodigiosin.