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      • KCI등재후보

        Tamarindus indica ameliorates behavioral and cytoarchitectural changes in the cerebellar cortex following prenatal aluminum chloride exposure in Wistar rats

        Ibe Michael Usman,Samuel Sunday Adebisi,Sunday Abraham Musa,Ibrahim Abdullahi Iliya,Victor Bassey Archibong,Ann Monima Lemuel,Keneth Iceland Kasozi 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.3

        Aluminium exposure has been linked with developmental neurotoxicity in humans and experimental animals. The study aimed to evaluate the ameliorative effect of Tamarindus indica on the developing cerebellar cortex, neurobehavior, and immunohistochemistry of the cerebellar cortex following prenatal aluminum chloride (AlCl 3) exposure. Pregnant timed Wistar rats were divided into 5 groups (n=4). Group I (negative control) was given distilled water, group II was treated with 200 mg/kg of AlCl 3 , group III were given 200 mg/kg of AlCl 3 and 400 mg/kg of ethyl acetate leaf fraction of Tamarindus indica (EATI), group IV were given 200 mg/kg of AlCl 3 and 800 mg/kg of EATI, and group V were treated with 200 mg/kg of AlCl 3 s/c and 300 mg/kg of vitamin E for 14 days (prenatal day 7–21) via the oral route. Male pups (n=6) were randomly selected and taken for neurobehavioral studies, and humanely sacrificed via intraperitoneal injection of thiopental sodium. The cerebellum was removed, fixed and tissue processed for histological and immunohistochemical studies. The results revealed that prenatal AlCl 3 exposure impacted neurodevelopment and neurobehaviour among exposed pups. Prenatal AlCl 3 exposure was marked with delayed cytoarchitectural development of the cerebellar cortex and increased GFAP expression in the cerebellar cortex. On the other hand, treatment with EATI and vitamin E were marked with significant improvements. The present study therefore concluded treatment with EATI shows an ameliorative effect to prenatal AlCl 3 exposure.

      • Determination and characterization of pharmaceuticals in sludge from municipal and livestock wastewater treatment plants

        Ekpeghere, Kalu Ibe,Lee, Ji-Woo,Kim, Hee-Young,Shin, Sun-Kyoung,Oh, Jeong-Eun Elsevier 2017 CHEMOSPHERE - Vol.168 No.-

        <P><B>Abstract</B></P> <P>This study investigated 24 pharmaceuticals compounds belonging to the classes of analgesics, stimulants, anti-seizures, non-steroidal anti-inflammatory drugs (NSAIDs), and antibiotics in the sludge of 12 municipal sewage treatment plants (S-sludge) and 4 livestock wastewater treatment plants (L-sludge) located across Korea. Over 70% of the target compounds were detected in at least one sample of S-sludge and L-sludge. The total concentration of the target pharmaceutical compounds detected in S-sludge was 2.622–422.8 mg kg<SUP>−1</SUP>and the most dominant compound was acetylsalicylic acid (ASA: 0.374–367.0 mg kg<SUP>−1</SUP>) whereas in L-sludge, the total concentration was 43.87–156.8 mg kg<SUP>−1</SUP>and the most abundant compound was oxytetracycline (OTC: 34.54–86.39 mg kg<SUP>−1</SUP>). Cluster analysis revealed two distinct groups: group A, which were S-sludge samples including ASA, carbamazepine (CBM), and others, and group B were L-sludge samples, dominated by antibiotics (CTC, OTC, LIN). The total daily load amount of the target pharmaceuticals in S-sludge was 0.010–268.9 kg day<SUP>−1</SUP> while the L-sludge was 0.021–0.529 kg day<SUP>−1</SUP>. The estimated amounts of the target pharmaceutical discharged from S-sludge and L-sludge into the Korean environment were 150.2 ± 47.94 ton yr<SUP>−1</SUP> and 15.05 ± 5.671 ton yr<SUP>−1</SUP> respectively, but the discharged amount of antibiotics from S-sludge (6.945 ton yr<SUP>−1</SUP>) was lower than that from L-sludge (9.234 ton yr<SUP>−1</SUP>).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pharmaceuticals in sewage and livestock sludge were studied. </LI> <LI> Acetylsalicylic acid was the most abundant pharmacuetical in the sewage sludge. </LI> <LI> Over 80% of the pharmacueticals observed in the livestock sludge were antibiotics. </LI> <LI> Estimated annual discharge amount of antibiotics from livestock sludge was 25% greater than sewage sludge. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • SCISCIESCOPUS

        Occurrence and distribution of carbamazepine, nicotine, estrogenic compounds, and their transformation products in wastewater from various treatment plants and the aquatic environment

        Ekpeghere, Kalu Ibe,Sim, Won-Jin,Lee, Heon-Jun,Oh, Jeong-Eun Elsevier 2018 Science of the Total Environment Vol.640 No.-

        <P><B>Abstract</B></P> <P>The concentrations and fates of carbamazepine and metabolites (CBMs), nicotine and metabolites (NCTs), estrogenic compounds and metabolites (Es) in various water samples were investigated. Different concentrations were found for water from different sources. The concentrations of these pharmaceuticals and personal care products and their metabolites in human waste treatment plant (HTP) influents (0.08–173 μg L<SUP>−1</SUP>) were higher than in the other influent samples and the lowest levels were observed in hospital wastewater treatment plant influents (0.03–7.33 μg L<SUP>−1</SUP>). The concentrations were higher in HTP effluents (0.01–11.2 μg L<SUP>−1</SUP>) than in the other effluent samples and lowest in sewage treatment plant effluents (0.003–1.26 μg L<SUP>−1</SUP>). The NCTs were the most frequently detected pharmaceuticals and personal care products (concentration range 0.05–89.6 μg L<SUP>−1</SUP>) in the wastewater treatment plant influents, but the CBMs were found at the highest concentrations (0.003–6.88 μg L<SUP>−1</SUP>). 10, 11-Dihydro-10, 11-dihydroxycarbamazepine was the most abundant of the CBMs in the wastewater treatment plants and water samples. <I>Trans</I>-3′-hydroxycotinine was dominant in the HTP and hospital wastewater treatment plant influents, whereas the parent NCT was dominant in the sewage treatment plant influents and in all the effluent and other water samples. Estriol was the dominant estrogenic compound in the HTP and hospital wastewater treatment plant influents. Estriol and estrone were found in many of the HTP influents, but estrone was dominant in the effluent and other water samples. The total removal efficiencies for the CBMs, NCTs, and estrogenic compounds for the treatment plants were −101% to 56%, 2.9%–99%, and >98%, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Higher concentrations of PPCPs and their metabolites were observed in HTPs than other WWTPs. </LI> <LI> The NCTs level was highest WWTPs in influents and CBMs were highest in effluents and water samples. </LI> <LI> CBM and NCT metabolite contributions were higher than parent compounds in most of the samples. </LI> <LI> E1 contribution was higher than other Es in effluents and water samples due to lower removal rate. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • SCOPUSKCI등재

        Anthropogenic determination and risk assessment of total petroleum hydrocarbons of ground water within Orji auto repair workshops, Owerri, Imo State, Nigeria

        Chukwu Fidelis Opia,Ibe Francis Chizoruo 환경독성보건학회 2024 환경독성보건학회지 Vol.39 No.1

        The research determined the anthropogenic source and risk evaluation of total petroleum hydrocarbons of some ground water sources within Orji auto repair workshops, Owerri, Imo State, Nigeria. The concentrations of total petroleum hydrocarbons in different groundwater samples within this vicinity were determined by selecting samples from five sampling locations using the liquid-liquid extraction process and gas chromatography fitted with a flame ionization detector. The concentrations of TPHs were determined with C15 (Pentadecane) and C30 (Triaconate) having the highest total concentrations in all sample locations of 29.67μg/mL and 23.17 μg/mL respectively while C13 (Tridecane) had the lowest combined concentration of 0.13 μg/mL with the presence of both low molecular weight and high molecular weight TPHs, an indication that the water samples had significant levels of carcinogens. Diagnostic ratio and correlation analyses of the samples analyzed showed contamination of the groundwater sources were both of petrogenic and pyrogenic sources. TPHs concentrations found in this study also revealed considerable amount of pollution with respect to contamination factors, pollution load index and degree of contamination. Elevated hazard index was equally observed for adults and children indicating a potential harm to the health of children and adults suggesting a higher risk of cancer from interaction with the polluted water. These results indicate that in remediation actions needs to be carried out before consumption to avoid fatal long term heath consequences.

      • Brominated flame retardants in marine environment focused on aquaculture area: Occurrence, source and bioaccumulation

        Gu, Seo-Yeon,Ekpeghere, Kalu Ibe,Kim, Hee-Young,Lee, In-Seok,Kim, Da-Hye,Choo, Gyojin,Oh, Jeong-Eun Elsevier 2017 The Science of the total environment Vol.601 No.-

        <P><B>Abstract</B></P> <P>Brominated flame retardants (BFRs), including polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA), and hexabromocyclododecanes (HBCDs), were investigated in bivalve (i.e., oyster (<I>Crassostrea gigas</I>) and mussel (<I>Mytilus coruscus</I>)), sediment, and seawater samples collected from aquaculture areas in South Korea to identify their occurrence, sources, and bioaccumulation. Among the studied chemicals, HBCDs predominated in bivalves and sediment, with concentrations of ND–67.52ng/g lipid weight and 3.47–168ng/g dry weight, respectively, while TBBPA was the highest contributor in seawater (ND–2.79ng/L). Compared with a non-aquaculture area, HBCD and PBDE concentrations were significantly higher in all matrices in the aquaculture area (Mann–Whitney <I>U</I> test, <I>p</I> <0.05), suggesting that sources may be located near or associated with the aquaculture areas, such as industrial complexes and expanded polystyrene buoys. Finally, the bioconcentration factor (BCF) and biota–sediment accumulation factor (BSAF) were estimated. Among the studied BFRs, BDE-47 (BCF: 1.70×10<SUP>6</SUP> L/kg; BSAF: 20.92) and α-HBCD (BCF: 1.05×10<SUP>6</SUP> L/kg; BSAF: 0.13) showed the highest accumulation potentials in bivalves.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This is the first field-based study to measure representative BFRs in biotic and abiotic matrices of aquaculture area. </LI> <LI> HBCDs were dominated in bivalves and sediment, meanwhile TBBPA was dominant in seawater. </LI> <LI> HBCDs were significantly higher in all matrices from aquaculture area than those from non-aquaculture area. </LI> <LI> Among the BFRs, BDE-47 and α-HBCD showed the highest BCFs and BSAFs in bivalves. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • SCISCIESCOPUS

        Effects of the summer holiday season on UV filter and illicit drug concentrations in the Korean wastewater system and aquatic environment

        Kim, Ki Yong,Ekpeghere, Kalu Ibe,Jeong, Hee-Jin,Oh, Jeong-Eun Elsevier 2017 Environmental pollution Vol.227 No.-

        <P><B>Abstract</B></P> <P>Seasonal variations in the concentrations of eight ultraviolet (UV) filters and 22 illicit drugs including their metabolites in the Korean aquatic environment were investigated. Seawater samples from three beaches, water samples from two rivers, and influents and effluents from three wastewater treatment plants were analyzed. The UV filter concentrations in the seawater, river water, and effluent samples were 39.4–296, 35.4–117, and 6.84–51.1 ng L<SUP>-1</SUP>, respectively. The total UV filter concentrations in the seawater samples were 1.9–4.4 times higher at the peak of the holiday season than outside the peak holiday season. An environmental risk assessment showed that ethylhexyl methoxy cinnamate (EHMC) could cause adverse effects on aquatic organisms in the seawater at the three beaches during the holiday period. Seven of the 22 target illicit drugs including their metabolites were detected in the wastewater influent samples, and the total illicit drug concentrations in the influent samples were 0.08–65.4 ng L<SUP>-1</SUP>. The estimated daily consumption rates for <I>cis</I>-tramadol (<I>Cis</I>-TRM), methamphetamine (MTP), meperidine (MEP), and codeine (COD) were 25.7–118.4, 13.8–36.1, 1.36–12.6, and 1.75–8.64 mg d<SUP>-1</SUP> (1000 people)<SUP>−1</SUP>, respectively. In popular vacation area, the illicit drug consumption rates (<I>Cis</I>-TRM, MTP and MEP) were 1.6–2.6 times higher at the peak of the summer holiday season than at the beginning of the summer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Eight ultraviolet (UV) filters and 22 illicit drugs were studied. </LI> <LI> Total UV filter concentrations in seawater were 1.9–4.4 times higher at the peak of summer vacation. </LI> <LI> Ethylhexyl methoxy cinnamate (EHMC) could cause adverse effects on aquatic organisms during this period. </LI> <LI> In holiday areas, illicit drug consumption rates were 1.6–2.6 times higher at the peak of the holiday period. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • KCI등재후보

        Assessment of the geo-environmental effects of activities of auto-mechanic workships at Alaoji Aba and Elekahia Port Harcourt, Niger Delta, Nigeria

        Nkanu Ernest Muze,Alexander Iheanyichukwu Opara,Francis Chizoruo Ibe,Okereke Chinwendu Njoku 환경독성보건학회 2020 환경독성보건학회지 Vol.35 No.2

        Geo-environmental assessment of activities of auto–mechanics at Alaoji Aba and Elekahia Port Harcourt, both in the Niger Delta region, Southern Nigeria were carried out with the main objective of determining the extent of soil contamination arising from anthropogenic activities within mechanic villages (MVs). Geochemical analysis of soil samples from the study area revealed that the concentrations of the trace metals ranged from <1 mg/kg for chromium (Cr) to 1,925 mg/kg for the lead (Pb). Soil analysis for polycyclic aromatic hydrocarbon (PAH) and total petroleum hydrocarbon (TPH) across the area revealed concentrations ranging from <0.02 to 1.80 mg/Kg and from <1.00 to 38,327 mg/kg respectively. Elevated levels of the heavy metals and TPH were observed at MV in Alaoji Aba when compared to MV in Elekahia Port Harcourt, and the control sites. These could be attributed to contamination due to the presence of these auto-mechanics in the area for over thirty years. The concentration of Pb and Cd recorded in some sample points were above USEPA (United State Environmental Protection Agency) and the National Environmental Standards and Regulations Enforcement Agency (NESREA) permissible limits. Results of PAH analysis showed the presence of naphthalene, phenanthrene, pyrene, fluorene, benzo(a)anthracene, acenaphthene, methylnaphthalene. Risk assessment analysis showed significant geo-accumulation values for Cd and Pb indicating heavy contamination. The monomial risk factor of the heavy metals in the MVs are in the order Cd >Pb>Cr, while potential ecological risk index analysis showed values indicating very high risk, considerable risk and a moderate risk to the area under study as well as the surrounding environment. These results suggest that the soils from the MVs which represent the mechanic workshops at Alaoji Aba and Elekahia Port Harcourt are considered to be of pollution concern due to elevated Pb and Cd levels. Hence, there is a serious need to regularly monitor the activities of auto-mechanics in the study area.

      • A new model for curbing filtrate loss in dynamic application of nano-treated aqueous mud systems

        Okoro, Emmanuel E.,Oladejo, Bukola R.,Sanni, Samuel E.,Obomanu, Tamunotonjo,Ibe, Amarachukwu A.,Orodu, Oyinkepreye D.,Olawole, Olukunle C. Techno-Press 2020 Advances in nano research Vol.9 No.1

        Filter cake formation during rotary drilling operation is an unavoidable scenario, hence there is need for constant improvement in the approaches used in monitoring the cake thickness growth in order to prevent drill-string sticking. This study proposes an improved model that predicts the growth of mud cake thickness overtime with the consideration of the addition of nanoparticles in the formulated drilling fluid system. Ferric oxide, titanium dioxide and copper oxide nanoparticles were used in varying amounts (2 g, 4 g and 6 g), and filtration data were obtained from the HPHT filtration test. The filter cakes formed were further analyzed with scanning electron microscope to obtain the morphological characteristics. The data obtained was used to validate the new filtrate loss model. This model specifically presents the concept of time variation in filter cake formation as against the previous works of constant and definite time. Regression coefficient which is a statistical measure was used to validate the new model and the predicted results were compared with the API model. The new model showed R<sup>2</sup> values of 99.9%, and the predictions from the proposed filtration model can be said to be more closely related to the experimental data than that predicted from the API model from the SSE and RMSE results.

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