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- 저자 : Huang Xinglong (검색결과 2 건)
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Yang-Jie Zhao,Huang Xingrui,Wen Fasheng,Huang Xinglong,Liu Zhixiao,Zhang Youxiang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Glutathione S-transferases (GSTs) are multifunctional detoxification enzymes which play important roles in protecting the organisms from environmental stress and are widely used as biomarkers for environmental bio monitoring studies. In this study, We investigated the GST activity in Protohermes costalis larvae, aquatic insects mainly found in oligotrophic fresh water environments, in response to cadmium (Cd) exposure. Eight GST genes in the larvae were identified and their expression patterns under Cd stress were determined by real-time quantitative polymerase chain reaction (RT-qPCR). The GST activity in the whole body was up-regulated by CdCl 2 in a dose-dependent manner and the midgut and malpighian tubules may be the main sites involved in GST activity regulation. The identified PcGSTs are members of cytosolic GST family containing the conserved glutathione-binding domain and substrate-binding domain. Six of them are highly enriched in malpighian tu bules, midgut or/and fat body. The expression level of PcGSTe1 was significantly up-regulated by 0.05, 0.1, 0.5, and 1 mM CdCl 2 treatments when compared with no Cd control. PcGSTs1 expression was significantly higher in 0.05 mM CdCl 2 and lower in 1 mM CdCl 2 compared with no Cd control. Other PcGSTs were up-regulated by different concentrations of CdCl 2 . Our results suggested that P. costalis midgut and malpighian tubules may be the main sites for GST activity regulation that induced by Cd in aqueous phase and increasing expression levels of different PcGST genes may be responsible for the GST activity up-regulation. Moreover, GST activity and gene expression in this insect may be used as biomarkers for future aquatic biomonitoring studies.
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Liu Chao,Liu Hongling,Wu Xinglong,Xiao Kejun,He Hengguo,Huang Qiong,Pu Deqiang 한국곤충학회 2022 Entomological Research Vol.52 No.4
The ladybeetle Coccinella septempunctata Linnaeus is an important natural enemy of aphids, scale insects, whitefly, and lepidopteran larvae. Mass rearing of this natural enemy is limited due to the lack of effective artificial feed. We compared the biological performance of C. septempunctata, reared on four artificial diets (A, B, C, and D), while the pea aphid Aphis craccivora served as a control treatment (CK). Results showed that the developmental time before emergence ranked from short to long follow as: CK (12.30d)<D(16.66d)<A(17.38d)<C (17.54d)<B (18.57d). The eclosion rate of larvae ranked from high to low follow as: CK (90.00%) > C (87.50%) = D (87.50%) > B(80.00%)> A (57.50%), and new adult weight from high to low follow as: CK (339.50 g*0.0001) > A (205.33 g*0.0001) > D (197.68 g*0.0001) > B (174.89 g*0.0001) > C (169.46 g*0.0001). The ratios of fecundity between the experimental group and the control group were 80.46% (A), 39.24% (B), 45.31% (C), and 53.02% (D). The hatch rates were 59.50% (A), 46.00% (B), 57.65% (C), 54.50% (D), and 53.88% (CK). The mortality of C. septempunctata adults fed on a combined artificial diet was higher than those fed on the control diet. Compared to the control diet, C. septempunctata did not significantly reduce oviposition when fed on artificial diet A. Therefore, diet A can be used in mass-rearing of C. septempunctata.
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