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- 저자 : Honggang Lv (검색결과 2 건)
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Cloning and Characterization of hydroxypyruvate isomerase (EC 5.3.1.22) gene in silkworm Bombyx mori
Lv, HongGang,Chen, KePing,Yao, Qin,Wang, Lin Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.17 No.2
The sequence of hydroxypyruvate isomerase gene was obtained in NCBI. In this study, the hydroxypyruvate isomerase gene of Bombyx.mori was identified and annotated with bioinformatics tools. The result was confirmed by RT-PCR, prokaryotic expression, mass spectrographic analysis and sub-cellular localization. The hydroxypyruvate isomerase cDNA comtains a 783bp ORF, and has 4 exons. The deduced protein has 260 amino acid residues with the predicted molecular weight of 29169.30 Da, isoelectric point of 6.10, and contains conserved PRK09997 and Hfi domains. The hydroxypyruvate isomerases of Nasonia vitripennis and Bombyx mori have a high homology. Through RTPCR analysis, we found that this transcript was present in testis, ovary, blood-lymph, fat body, midgut, silk gland and tuba Malpighii. This protein was located in cytoplasm through immunohistochemistry. We submitted the cloned gene under the accession number EU344910. The enzyme has been classified under accession number EC 5.3.1.22.
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Saw-Tooth Chip Formation and Its Effect on Cutting Force Fluctuation in Turning of Inconel 718
Song Zhan,Jianfeng Li,Xiaoli Zhu,Honggang Lv 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.
Chip formation and its morphology are important features of metal cutting, and they can yield much more important information on the cutting operations. Meantime, the cutting forces are the most commonly used on-line indicators to monitor the cutting process. In this research, the formation of the saw-tooth chips has been investigated in association with the fluctuation of the cutting force components under different cutting parameters in turning of Inconel 718 with (Ti, Al)N/TiN coated cutting tools under dry cutting condition. By means of the optical microscopy and the scanning electron microscopy (SEM), the saw-tooth chips are observed at high cutting speeds, which directly cause the cutting force components to fluctuate. Based on the frequency analysis, the segmentation frequencies of the saw-tooth chips and the fluctuation frequency of the cutting forces are very close to each other. It indicates that the formation of the saw-tooth chips is one of the important factors leading to the periodical fluctuation of the cutting force components, even though the cutting parameters all keep constant in turning of Inconel 718.
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