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Tadee, Pakpoom,Tadee, Phacharaporn,Hitchings, Matthew D.,Pascoe, Ben,Sheppard, Samuel K.,Patchanee, Prapas The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.2
Non-typhoidal Salmonella is one of the main pathogens causing food-borne illness in humans, with up to 20% of cases resulting from consumption of pork products. Over the gastroenteritis signs, multidrug resistant Salmonella has arisen. In this study, pan-susceptible phenotypic strains of Salmonella enterica serotype Weltevreden recovered from pig production chain in Chiang Mai, Thailand during 2012-2014 were chosen for analysis. The aim of this study was to use whole genome sequencing (WGS) data with an emphasis on antimicrobial resistance gene investigation to assess their pathogenic potential and genetic diversity determination based on whole genome Multilocus Sequence Typing (wgMLST) to expand epidemiological knowledge and to provide additional guidance for disease control. Analyis using ResFinder 3.0 for WGS database tracing found that one of pan-susceptible phenotypic strain carried five classes of resistance genes: aminoglycoside, beta-lactam, phenicol, sulfonamide, and tetracycline associated genes. Twenty four and 36 loci differences were detected by core genome Multilocus Sequence Typing (cgMLST) and pan genome Multilocus Sequence Typing (pgMLST), respectively, in two matching strains (44/13 vs A543057 and A543056 vs 204/13) initially assigned by conventional MLST and Pulsed-field Gel Electrophoresis (PFGE). One hundread percent discriminant ability can be achieved using the wgMLST technique. WGS is currently the ultimate molecular technique for various in-depth studies. As the findings stated above, a new of "gold standard typing method era" for routine works in genome study is being set.
Pakpoom Tadee,Phacharaporn Tadee,Matthew D. Hitchings,Ben Pascoe,Samuel K. Sheppard,Prapas Patchanee 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.2
Non-typhoidal Salmonella is one of the main pathogens causing food-borne illness in humans, with up to 20% of cases resulting from consumption of pork products. Over the gastroenteritis signs, multidrug resistant Salmonella has arisen. In this study, pan-susceptible phenotypic strains of Salmonella enterica serotype Weltevreden recovered from pig production chain in Chiang Mai, Thailand during 2012−2014 were chosen for analysis. The aim of this study was to use whole genome sequencing (WGS) data with an emphasis on antimicrobial resistance gene investigation to assess their pathogenic potential and genetic diversity determination based on whole genome Multilocus Sequence Typing (wgMLST) to expand epidemiological knowledge and to provide additional guidance for disease control. Analyis using ResFinder 3.0 for WGS database tracing found that one of pan-susceptible phenotypic strain carried five classes of resistance genes: aminoglycoside, beta-lactam, phenicol, sulfonamide, and tetracycline associated genes. Twenty four and 36 loci differences were detected by core genome Multilocus Sequence Typing (cgMLST) and pan genome Multilocus Sequence Typing (pgMLST), respectively, in two matching strains (44/13 vs A543057 and A543056 vs 204/13) initially assigned by conventional MLST and Pulsed-field Gel Electrophoresis (PFGE). One hundread percent discriminant ability can be achieved using the wgMLST technique. WGS is currently the ultimate molecular technique for various in-depth studies. As the findings stated above, a new of “gold standard typing method era” for routine works in genome study is being set.
LOW RANK ORTHOGONAL BUNDLES AND QUADRIC FIBRATIONS
Insong Choe,George H. Hitching Korean Mathematical Society 2023 대한수학회지 Vol.60 No.6
Let C be a curve and V → C an orthogonal vector bundle of rank r. For r ≤ 6, the structure of V can be described using tensor, symmetric and exterior products of bundles of lower rank, essentially due to the existence of exceptional isomorphisms between Spin(r, ℂ) and other groups for these r. We analyze these structures in detail, and in particular use them to describe moduli spaces of orthogonal bundles. Furthermore, the locus of isotropic vectors in V defines a quadric subfibration Q<sub>V</sub> ⊂ ℙV . Using familiar results on quadrics of low dimension, we exhibit isomorphisms between isotropic Quot schemes of V and certain ordinary Quot schemes of line subbundles. In particular, for r ≤ 6 this gives a method for enumerating the isotropic subbundles of maximal degree of a general V , when there are finitely many.