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Civic Engagement of Older Adults in a Northern City of Canada
Kelly Gingrich, Parveen Nangia 한국캐나다학회 2023 Asia-Pacific Journal of Canadian Studies (APJCS) Vol.29 No.2
Civic engagement is considered virtuous for the citizens as it enhances individual and social well-being. With age, interest and capability of participation in civic activities may decline. This study is part of a larger study on quality of life of older adults in Greater Sudbury. It is based on interviews with 20 citizens over the age of 80 years and examines their civic engagement. The participants were selected through a combination of purposive and snowball sampling designs. During the face-to-face interviews, they were asked questions about the rights of citizens in general and their own involvement in political and voluntary activities. The findings of the study indicate that the older adults in the city consider it their duty to cast vote in elections at all levels, however, they are disappointed with the politicians who either fail to keep their promises or adopt policies that counter social welfare, particularly the welfare of older adults. They also expressed their concerns about many local issues. Their participation in voluntary activities waned down to mostly helping their family and friends, though some of them still supported their community in different ways. Their active participation in civic activities could be enhanced by overcoming some of the age-related challenges.
Rapid Variable-Number Tandem-Repeat Genotyping for Mycobacterium leprae Clinical Specimens
Kimura, M.,Sakamuri, R. M.,Groathouse, N. A.,Rivoire, B. L.,Gingrich, D.,Krueger-Koplin, S.,Cho, S.-N.,Brennan, P. J.,Vissa, V. American Society for Microbiology 2009 Journal of clinical microbiology Vol.47 No.6
<P>Mycobacterium leprae is the noncultivable pathogen of leprosy. Since the genome sequence of an isolate of M. leprae has become available, multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) has been explored as a tool for strain typing and identification of chains of transmission of leprosy. In order to discover VNTRs and develop methods transferable to clinical samples, MLVA was applied to a global collection of M. leprae isolates derived from leprosy patients and propagated in armadillo hosts. PCR amplification, agarose gel electrophoresis, and sequencing methods were applied to DNA extracts from these infected armadillo tissues (n = 21). We identified polymorphisms in 15 out of 25 short-tandem-repeat (STR) loci previously selected by in silico analyses of the M. leprae genome. We then developed multiplex PCR for amplification of these 15 loci in four separate PCRs suitable for fluorescent fragment length analysis and demonstrated STR profiles highly concordant with those from the sequencing methods. Subsequently, we extended this method to DNA extracts from human clinical specimens, such as skin biopsy specimens (n = 30). With these techniques, mapping of multiple loci and differentiation of genotypes have been possible using total DNA extracts from limited amounts of clinical samples at a reduced cost and with less time. These practical methods are therefore available and applicable to answer focused epidemiological questions and to allow monitoring of the transmission of M. leprae in different countries where leprosy is endemic.</P>