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Myung Sook Lee,Hae Young Oh,Jae Han Son,George Fedak,Nam Soo Kim 한국유전학회 2007 Genes & Genomics Vol.29 No.4
Molecular cytogenetic analysis was carried out in the ancestral genome donors to polyploidy wheats, T. turgidum, and T. aestivum, with a fungus derived TC/AG microsatellite sequence, pKFJ660. The 45S rRNA gene was also used for chromosome identification in FISH analysis. The A and D genome donors, T. monococcum and T. tauchii, showed no hybridization signals with the pKFJ660. The hybridization sites were variable in the chromosomes of S genome Sitopsis species which were the putative B genome donors to the polyploid wheats. Four pairs of chromosomes showed pKFJ660 hybridization signals in T. turgidum (1B, 3B, 5B and 6B) and T. aestivum (3B, 4B, 5B, and 6B). Differences of hybridization signal intensities and FISH signal distributions were high among the S genome diploids and polyploid wheats, which hindered providing unequivocal explanations for the wheat evolution with this microsatellite sequence. With the evidences obtained, it seemed to be evident that the pKFJ660 microsatellite sequence had undergone restructuring and reorganization in its sequence along the chromosomes during wheat evolution. Since the pKFJ660 contained AluI transposon related direct repeat, possibilities of transposon mediated genome instability by allopolyploidization were discussed.
Jirui Wang,Yaxi Liu,Yan Wang,Zhenhong Chen,Shuai Dai,Wenguang Cao,George Fedak,Xiujing Lan,Yuming Wei,Dengcai Liu,Youliang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2
Pre-harvest sprouting (PHS) in bread wheat is a major abiotic constraint reducing yield and influencing the production of high quality grain. In China both spring and winter wheat regions are affected by PHS. Sichuan lies in southwest China,where the most of rainfall occurs during April to September when wheat is harvested. The present investigation was conducted to identify the allelic variability of Vp1, a gene that plays a role in maintenance and induction of dormancy,among Sichuan landraces and recent cultivars with different dormancy levels and to find potential sources of PHS resistance for breeding. Sichuan landrace and cultivar wheat accessions had a wide range of dormancy levels. The average germination index (GI) of Sichuan landrace accessions was 0.232, whereas at 0.674 it was much higher for cultivars. The different dormancy levels between landraces and cultivars indicated that pre-harvest sprouting resistance might have been neglected in recent Sichuan wheat breeding programs. The average GI of white grained accessions was higher than for red grained accessions. Particular Vp-1B gene fragments were specific in landraces or cultivars and in white or red grained accessions. The results indicated that Vp-1B markers could be used to distinguish cultivars and landraces. Significant relationships between certain Vp-1B allelesand GI of Sichuan wheat accessions were shown by Spearman’s rank correlation analysis.