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Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology
Bak Seong-Kun,Seong Wonjae,Rha Eugene,Lee Hyewon,Kim Seong Keun,Kwon Kil Koang,Kim Haseong,Lee Seung-Goo 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.8
This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genotyping. We confirmed that the fluorescence intensities of colonies on plates were comparable to fluorescence at the single-cell level from a high-end, flow-cytometry device and developed a high-throughput image analysis pipeline. The barcode tagging-based long-read sequencing technique enabled rapid identification of all DNA parts and their combinations with a single sequencing experiment. Using our techniques, forty-four DNA parts (21 promoters and 23 RBSs) were successfully characterized in 72 h without any automated equipment. We anticipate that this high-throughput and easy-to-use part characterization technique will contribute to increasing part diversity and be useful for building genetic circuits and metabolic pathways in synthetic biology.
A Case Report : Bilateral tension pneumothoraces during minimally invasive parathyroidectomy
Andres Falabella,Michael W Lew,Walter L Chang,Eugene L Bak 대한마취과학회 2009 Korean Journal of Anesthesiology Vol.57 No.2
Minimally invasive parathyroidectomy, a new technique for the surgical management of parathyroid disease, is gaining popularity. The smaller incision in the neck results in better cosmetic results and patient satisfaction. Despite a low incidence of complications, the anesthesiologist should be aware and prepared to manage life saving situations. We describe a case of bilateral tension pneumothoraces during minimally invasive parathyroidectomy. (Korean J Anesthesiol 2009;57:246∼8)
Kim, Sunghan,Yoo, Kyu-Cheul,Lee, Jae Il,Khim, Boo-Keun,Bak, Young-Suk,Lee, Min Kyung,Lee, Jongmin,Domack, Eugene W.,Christ, Andrew J.,Yoon, Ho Il Elsevier 2018 Quaternary science reviews Vol.192 No.-
<P><B>Abstract</B></P> <P>Paleoceanographic changes in response to Holocene climate variability in Bigo Bay, west Antarctic Peninsula (WAP) were reconstructed through geochemical, isotopic, sedimentological, and microfossil analysis. Core WAP13-GC47 is composed of 4 lithologic units. Unit 4 was deposited under ice shelf settings. Unit 3 represents the mid-Holocene open marine conditions. Unit 2 indicates lateral sediment transport by a glacier advance during the Neoglacial period. The chronological contrast between the timing of open marine conditions at core WAP13-GC47 (<I>ca</I>. 7060 cal. yr BP at 540 cm) and the ages of calcareous shell fragments (<I>ca</I>. 8500 cal. yr BP) in Unit 2b suggests sediment reworking during the Neoglacial period. Unit 1 was deposited during the Medieval Warm Period (MWP) and the Little Ice Age (LIA). Surface water productivity, represented by biogenic opal and total organic carbon (TOC) concentrations, increased and bulk δ<SUP>15</SUP>N (nitrate utilization) decreased during the warmer early to middle Holocene and the MWP. In contrast, surface water productivity decreased with increased bulk δ<SUP>15</SUP>N during the colder Neoglacial period and LIA in Bigo Bay. The nitrate utilization was enhanced during cold periods in association with strong surface water stratification resulting from increased sea ice meltwater discharge or proximity to an ice shelf calving front in Bigo Bay. Reduced nitrate utilization during warm periods is related to weak stratification induced by less sea ice meltwater input and stronger Circumpolar Deep Water influence.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced stratification caused high nitrate utilization during cold periods. </LI> <LI> Low nitrate utilization is ascribed to CDW input/less sea-ice melt water during warm periods. </LI> <LI> Surface water productivity was high (low) during warm (cold) periods with opposite patterns of nitrate utilization. </LI> <LI> Surface-deep water mass exchange in the WAP fjord is influenced by ice shelf/sea-ice variation. </LI> </UL> </P>