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      • Interactive effect of indole-3-acetic acid and diethyl aminoethyl hexanoate on the growth and fatty acid content of some microalgae for biodiesel production

        Salama, El-Sayed,Jeon, Byong-Hun,Chang, Soon Woong,Lee, Sang-hun,Roh, Hyun-Seog,Yang, Il-Seung,Kurade, Mayur B.,El-Dalatony, Marwa M.,Kim, Do-Hyeon,Kim, Ki-Hyun,Kim, Sunjoon Elsevier 2017 Journal of cleaner production Vol.168 No.-

        <P><B>Abstract</B></P> <P>Enhancement of microalgal growth and fatty acid production is essential for development of a microalgae-based biodiesel production platform. Three different microalgal species (<I>Scenedesmus obliquus</I> GU732418, <I>Ourococcus multisporus</I> GU732424 and <I>Chlorella vulgaris</I> FR751187) were individually cultivated in media containing both indole-3-acetic acid (IAA) and diethyl aminoethyl hexanoate (DAH) at concentrations of 10<SUP>−8</SUP>−10<SUP>−4</SUP> M. Combined phytohormones (10<SUP>−8</SUP> to 10<SUP>−5</SUP> M) increased the growth of all three species compared to growth in media without phytohormones. IAA and DAH supported the maximum growth of <I>S</I>. <I>obliquusi</I> (38.12 × 10<SUP>6</SUP> cells mL<SUP>−1</SUP>) at 10<SUP>−8</SUP> M, <I>O</I>. <I>multisporus</I> (85.89 × 10<SUP>6</SUP> cells mL<SUP>−1</SUP>) at 10<SUP>−6</SUP> M, and <I>C. vulgaris</I> (4.09 × 10<SUP>6</SUP> cells mL<SUP>−1</SUP>) at 10<SUP>−5</SUP> M. Addition of 10<SUP>−7</SUP> M IAA and DAH also assisted the removal of Zn<SUP>2+</SUP> (97%), K<SUP>+</SUP> (88%) and Mg<SUP>2+</SUP> (99%) from the media by <I>S</I>. <I>obliquus</I>. The highest removal of Zn<SUP>2+</SUP>, K<SUP>+</SUP>, and Mg<SUP>2+</SUP> by <I>C</I>. <I>vulgaris</I> was achieved at 10<SUP>−5</SUP> M IAA and DAH. Under all experimental conditions (10<SUP>−8</SUP>−10<SUP>−4</SUP> M IAA and DAH) the amounts of poly-unsaturated fatty acids were significantly increased. Palmitic acid, linoleic acid and γ-linolenic acid were the major fatty acids, accounting for 11.75–21.55%, 2.55–6.73%, and 52.93–75.89% of the total fatty acid content, respectively. The fatty acids that accumulated in <I>O</I>. <I>multisporus</I> and <I>C</I>. <I>vulgaris</I> were found to be suitable for production of high quality biodiesel with characteristics equivalent to crop seed oil-derived biodiesel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> IAA and DAH in the range of 10<SUP>−8</SUP>−10<SUP>−5</SUP> M enhanced the growth of algae. </LI> <LI> PUFAs was increased by growth in medium containing both IAA and DAH. </LI> <LI> Accumulated fatty acids in algae are suitable for production of high quality biodiesel. </LI> </UL> </P>

      • SCIESCOPUSKCI등재

        Cultivation of a New Microalga, Micractinium reisseri, in Municipal Wastewater for Nutrient Removal, Biomass, Lipid, and Fatty Acid Production

        Abou-Shanab, Reda A.I.,El-Dalatony, Marwa M.,EL-Sheekh, Mostafa M.,Ji, Min-Kyu,Salama, El-Sayed,Kabra, Akhil N.,Jeon, Byong-Hun 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3

        Coupling of advanced wastewater treatment with microalgae cultivation for low-cost lipid production was demonstrated in this study. The microalgal species Micractinium reisseri and Scenedesmus obliquus were isolated from municipal wastewater mixed with agricultural drainage. M. reisseri was selected based on the growth rate and cultivated in municipal wastewater (influent, secondary and tertiary effluents) which varied in nutrient concentration. M. reisseri showed an optimal specific growth rate (${\mu}_opt$) of 1.15, 1.04, and 1.01 1/day for the influent and the secondary and tertiary effluents, respectively. Secondary effluent supported the highest phosphorus removal (94%) and saturated fatty acid content (40%). The highest lipid content (40%), unsaturated fatty acid content, including monounsaturated and polyunsaturated fatty acids (66%), and nitrogen removal (80%) were observed for tertiary effluent. Fatty acids accumulating in the microalgal biomass (M. reisseri) were mainly composed of palmitic acid, oleic acid, linoleic acid, and ${\alpha}$-linolenic acid. Cultivation of M. reisseri using municipal wastewater served a dual function of nutrient removal and biofuel feedstock generation.

      • SCISCIESCOPUS

        Whole conversion of microalgal biomass into biofuels through successive high-throughput fermentation

        El-Dalatony, Marwa M.,Salama, El-Sayed,Kurade, Mayur B.,Kim, Kyoung-Yeol,Govindwar, Sanjay P.,Kim, Jung Rae,Kwon, Eilhann E.,Min, Booki,Jang, Min,Oh, Sang-Eun,Chang, Soon Woong,Jeon, Byong-Hun Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.360 No.-

        <P><B>Abstract</B></P> <P>Microalgae represent a promising feedstock for biofuel production. However, the energy efficiency of microalgal pretreatment and conversion technologies needs to be improved to meet the economic viability. Herein, we introduce a novel integrated approach to achieve unprecedented energy conversion efficiency (46%) of microalgal biomass (<I>Chlamydomonas mexicana</I>). A successive high-throughput fermentation followed by transesterification were employed. This process provided a platform for maximum recovery of energy carriers from biomass utilization (89%). Serial fermentations were implemented for thorough utilization of the biomass constituents, starting with carbohydrate, followed by protein to derive ethanol (C2) and higher alcohols (C3–C5), respectively. Lipid was the dominant component after the previous fermentation, which was converted to biodiesel via transesterification process. Successive fermentations served as a bio-pretreatment to enhance the bioavailability of the leftover protein and lipid, which minimized the use of expensive and laborious methods for their extraction from the microalgal biomass. The proposed serial fermentation process would maximize the utilization of biomasses for biofuel production, with minimum leftover (11%).</P> <P><B>Highlights</B></P> <P> <UL> <LI> High throughput fermentations achieved 46% energy recovery from microalgae. </LI> <LI> Successive fermentations served as a biopretreatment to enhance the accessibility. </LI> <LI> 89% of biomass was converted into biofuels with less production of waste. </LI> <LI> Fermentation of the leftover protein produced 0.37 g-higher alcohols/g-amino acids. </LI> <LI> Transesterification of the remaining lipids produced 0.5 g-biodiesel/g-fatty acids. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • Biological Conversion of Amino Acids to Higher Alcohols

        El-Dalatony, Marwa M.,Saha, Shouvik,Govindwar, Sanjay P.,Abou-Shanab, Reda A.I.,Jeon, Byong-Hun Elsevier 2019 Trends in biotechnology Vol.37 No.8

        <P>‘Higher’ alcohols, which contain more than two carbons, have a higher boiling point, higher cetane number, and higher energy density than ethanol. Blends of biodiesel and higher alcohols can be used in internal combustion engines as next-generation biofuels without any modification and are minimally corrosive over extensive use. Producing higher alcohols from biomass involves fermenting and metabolizing amino acids. In this review, we describe the pathways and regulatory mechanisms involved in amino acid bioprocessing to produce higher alcohols and the effects of amino acid supplementation as a nitrogen source for higher alcohol production. We also discuss the most recent approaches to improve higher alcohol production via genetic engineering technologies for three microorganisms: <I>Saccharomyces cerevisiae</I>, <I>Clostridium</I> spp., and <I>Escherichia coli</I>.</P> <P><B>Highlights</B></P> <P>Proteins are polymers of various amino acids, connected via peptide bonds and classified as a major feedstock for bioenergy production. Higher alcohols are high-density alternative fuels that increase the longevity of transportation fuels.</P> <P>Proteins have a significant role in the fermentation process by providing amino acids for the growth of microorganisms, and enhancement of sugar permeability, in carbohydrate-rich sources.</P> <P>Due to the environmental and economic advantages of recombinant DNA technology, fermentation is the most used process for industrial-scale alcohol production. Applying this technology to higher alcohols can significantly improve industrialization for advanced fuel production.</P> <P>Extraction techniques are used to separate and mitigate the toxicity of alcohols produced in the fermentation broth to maintain the microbial cell viability for longer.</P>

      • Enhancement of microalgal growth and biocomponent-based transformations for improved biofuel recovery: A review

        Salama, El-Sayed,Hwang, Jae-Hoon,El-Dalatony, Marwa M.,Kurade, Mayur B.,Kabra, Akhil N.,Abou-Shanab, Reda A.I.,Kim, Ki-Hyun,Yang, Il-Seung,Govindwar, Sanjay P.,Kim, Sunjoon,Jeon, Byong-Hun Elsevier 2018 Bioresource technology Vol.258 No.-

        <P><B>Abstract</B></P> <P>Microalgal biomass has received much attention as feedstock for biofuel production due to its capacity to accumulate a substantial amount of biocomponents (including lipid, carbohydrate, and protein), high growth rate, and environmental benefit. However, commercial realization of microalgal biofuel is a challenge due to its low biomass production and insufficient technology for complete utilization of biomass. Recently, advanced strategies have been explored to overcome the challenges of conventional approaches and to achieve maximum possible outcomes in terms of growth. These strategies include a combination of stress factors; co-culturing with other microorganisms; and addition of salts, flue gases, and phytohormones. This review summarizes the recent progress in the application of single and combined abiotic stress conditions to stimulate microalgal growth and its biocomponents. An innovative schematic model is presented of the biomass-energy conversion pathway that proposes the transformation of all potential biocomponents of microalgae into biofuels.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Improvement of biochemical components using combined abiotic stress. </LI> <LI> Microalgae and their properties vis-à-vis biofuel production. </LI> <LI> Transformation of all potential biochemical components into biofuels. </LI> </UL> </P>

      • Recent progress in microalgal biomass production coupled with wastewater treatment for biofuel generation

        Salama, El-Sayed,Kurade, Mayur B.,Abou-Shanab, Reda A.I.,El-Dalatony, Marwa M.,Yang, Il-Seung,Min, Booki,Jeon, Byong-Hun Elsevier 2017 RENEWABLE & SUSTAINABLE ENERGY REVIEWS Vol.79 No.-

        <P><B>Abstract</B></P> <P>Microalgae are a potential source of sustainable biomass feedstock for biofuel generation, and can proliferate under versatile environmental conditions. Mass cultivation of microalgae is the most overpriced and technically challenging step in microalgal biofuel generation. Wastewater is an available source of the water plus nutrients necessary for algae cultivation. Microalgae provide a cost-effective and sustainable means of advanced (waste)water treatment with the simultaneous production of commercially valuable products. Microalgae show higher efficiency in nutrient removal than other microorganisms because the nutrients (ammonia, nitrate, phosphate, urea and trace elements) present in various wastewaters are essential for microalgal growth. Potential progress in the area of microalgal cultivation coupled with wastewater treatment in open and closed systems has led to an improvement in algal biomass production. However, significant efforts are still required for the development and optimization of a coupled system to simultaneously generate biomass and treat wastewater. In this review, the systematic description of the technologies required for the successful integration of wastewater treatment and cultivation of microalgae for biomass production toward biofuel generation was discussed. It deeply reviews the microalgae-mediated treatment of different wastewaters (including municipal, piggery/swine, industrial, and anaerobic wastewater), and highlight the wastewater characteristics suitable for microalgae cultivation. Various pretreatment methods (such as filtration, autoclaving, UV application, and dilution) needed for wastewater prior to its use for microalgae cultivation have been discussed. The selection of potential microalgae species that can grow in wastewater and generate a large amount of biomass has been considered. Discussion on microalgal cultivation systems (including raceways, photobioreactors, turf scrubbers, and hybrid systems) that use wastewater, evaluating the capital expenditures (CAPEX) and operational expenditures (OPEX) of each system was reported. In view of the limitations of recent studies, the future directions for integrated wastewater treatment and microalgae biomass production for industrial applications were suggested.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Challenges in using wastewater for microalgae cultivation and biomass production. </LI> <LI> Treatment of different wastewaters and reuse of the treated water. </LI> <LI> Recovery of valuable nutrients (N/P) and removal of organic pollutants. </LI> <LI> Application of wastewater in raceways, photobioreactors, turf scrubbers, and hybrid systems. </LI> <LI> Genetically engineered microalgae for efficient wastewater treatment. </LI> </UL> </P>

      • SCISCIESCOPUS

        Biocomponent-based microalgal transformations into biofuels during the pretreatment and fermentation process

        Ha, Geon-Soo,El-Dalatony, Marwa M.,Kim, Do-Hyeon,Salama, El-Sayed,Kurade, Mayur B.,Roh, Hyun-Seog,El-Fatah Abomohra, Abd,Jeon, Byong-Hun Elsevier 2020 Bioresource technology Vol.302 No.-

        <P><B>Abstract</B></P> <P>Microalgal cell wall integrity and composition have a significant impact on the fermentation process and biofuel recovery. In this study, various biofuels (bioethanol, higher alcohols (C3-C5), and biodiesel) were produced by the fermentation of carbohydrates and proteins, and transesterification of lipids from three different microalgal strains (<I>Pseudochlorella</I> sp., <I>Chlamydomonas mexicana</I>, and <I>Chlamydomonas pitschmannii</I>), each possessing different proportions of bioconstituents (carbohydrates, proteins, and lipids). Changes in the cell wall structure and thickness were observed before and after fermentation using transmission electron microscopy. <I>Pseudochlorella</I> sp. showed the highest yields of bioethanol (0.45 g-ethanol/g-carbohydrates), higher alcohols (0.44 g-higher alcohols/g-proteins), and biodiesel (0.55 g-biodiesel/g-lipids), which consequently revealed a maximum energy recovery (42%) from whole constituents. This study suggests that different physiological properties, including cell wall thickness and the proportion of bioconstituents in microalgae, could have a significant impact on the pretreatment and fermentation efficiencies for biofuels production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Algal biocomponents has an influence on pretreatment and fermentation efficiency. </LI> <LI> Highest biofuels yield (0.44–0.55 g/g) was obtained from <I>Pseudochlorella</I> sp. </LI> <LI> Cell wall thickness dependent on bioconstituents and affected biofuels yield. </LI> <LI> Highest total energy recovery (42%) was achieved using suitable <I>Pseudochlorella</I> sp. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • SCISCIESCOPUS

        Harvest of electrical energy from fermented microalgal residue using a microbial fuel cell

        Song, Young Eun,El-Dalatony, Marwa M.,Kim, Changman,Kurade, Mayur B.,Jeon, Byong-Hun,Kim, Jung Rae Elsevier 2019 International journal of hydrogen energy Vol.44 No.4

        <P><B>Abstract</B></P> <P>The application of microalgal biomass for fermentation has been highlighted as a means of producing a range of value-added biofuels and chemicals. On the other hand, the microalgal residue from the fermentation process still contains as much as 50% organic contaminants, which can be a valuable substrate for further bioenergy recovery. In this study, a microbial fuel cell and automatic external load control by maximum power point tracking (MPPT) were implemented to harvest the electrical energy from waste fermented microalgal residue (FMR). The MFC with MPPT produced the highest amount of energy (1.82 kJ/L) compared to the other MFCs with fixed resistances: 0.98 (1000 Ω), 1.16 (500 Ω), and 1.17 kJ/L (300 Ω). The MFC with MPPT also showed the highest maximum power density (88.6 mW/m<SUP>2</SUP>) and COD removal efficiency (620.0 mg COD/L removal with 85% removal efficiency). The implementation of MPPT gained an approximate 12.9% energy yield compared to the previous fermentation stage. These results suggest that FMR can be an appropriate feedstock for electrical energy recovery using MFCs, and the combined fermentation and MFC system improves significantly the energy recovery and treatment efficiency from FMR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The combination of fermentation and MFC improves energy recovery. </LI> <LI> Fermented microalgal residue (FMR) was used as the feedstock for MFCs. </LI> <LI> Automatic load control with maximum power point tracking (MPPT) was implemented. </LI> <LI> The MPPT-MFC showed the highest energy production (1.82 kJ/L) with a 12.9% yield. </LI> <LI> The combined system enhanced the energy recovery and treatment efficiency. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • Perspective on anaerobic digestion for biomethanation in cold environments

        Dev, Subhabrata,Saha, Shouvik,Kurade, Mayur B.,Salama, El-Sayed,El-Dalatony, Marwa M.,Ha, Geon-Soo,Chang, Soon Woong,Jeon, Byong-Hun Elsevier 2019 RENEWABLE & SUSTAINABLE ENERGY REVIEWS Vol.103 No.-

        <P><B>Abstract</B></P> <P>The anaerobic digestion (AD) has become an important part of the wastewater treatment plants that regulates the sustainable management of organic wastes with simultaneous production of bioenergy. AD at low temperatures using psychrophilic anaerobes with optimum growth temperatures < 20 °C has gained significant attention for improvement of biogas productivity in cold regions. The present review discusses the detailed characteristics of psychrophilic anaerobes, and how the properties of those particular psychrophiles can be utilized towards the cost-effective production of methane at cold environment. The different challenges for AD at low temperature have been described thoroughly. The various strategies such as (a) adaptation of microbial community, (b) optimization of operational parameters, (c) utilization of specialized biodigester design, and (d) modification of downstream process to improve the AD and biomethane production in cold environments have also been summarized. The present review proposes the future technological developments which should be aimed at effective performance of anaerobic digesters to improve biomethanation in cold regions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Psychrophilic AD is an energy efficient process for biomethanation in cold regions. </LI> <LI> Alterations of cellular physiology increases the adaptive response in psychrophiles. </LI> <LI> Cold adaptation of inoculum and process optimization could improve psychrophilic AD. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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