http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Daisy Pérez-Brito,Alberto Cortes-Velázquez,Teresita Valencia-Yah,Anuar Magaña-Álvarez,Cuauhtémoc Navarro,Blanca Moreno,Steven Quiroga,Raúl Tapia-Tussell 한국미생물학회 2018 The journal of microbiology Vol.56 No.11
Mexico is one of the five largest producers of papaya worldwide, but losses caused by pathogens, mainly fungus, at the pre- and post-harvest stages are often more than 50% of the crop. Papaya anthracnose, caused by three different species of the Colletotrichum genus in Mexico, occupies a preponderant place in this problem. Although two of these species, C. gloeosporiodes and C. truncatum, have been characterized morphologically and genotypically, this has not occurred with C. magnum, the third species involved, about which there is very little information. Because of this, it is vital to know its genetic characterization, much more so considering that the studies carried out on the other two species reveal a wide genetic diversity, differences in pathogenicity and in the response to fungicides of the different strains characterized. In this work, Colletotrichum spp. isolates were collected at different papaya orchards in the south-southeast of Mexico. C. magnum isolates identified by species-specific primers were characterized by morphological and molecular approaches. Differences in colony characteristics resulted in five morphological groups. AP-PCR, DAMD and ISSR markers were found to be very efficient for revealing the interspecific variability of this species. The high genetic variability found in the accessions of C. magnum was linked to the geographical area where they were collected. Isolates from Chiapas State were the most variable, showing point mutations in the ITS1- ITS2 region. These results will enable a better phytosanitary management of anthracnose in papaya in this region of Mexico.
Daisy Pérez-Brito,Anuar Magaña-Alvarez,Patricia Lappe-Oliveras,Alberto Cortes-Velazquez,Claudia Torres-Calzada,Teófilo Herrera- Suarez,Alfonso Larqué-Saavedra,Raul Tapia-Tussell 한국미생물학회 2015 The journal of microbiology Vol.53 No.1
This study characterized Clavispora lusitaniae strains isolatedfrom different stages of the processing and early fermentationof a henequen (Agave fourcroydes) spirit produced inYucatan, Mexico using a molecular technique. Sixteen strainsidentified based on morphological features, obtained fromdifferent substrates, were typed molecularly. Nine differentversions of the divergent D1/D2 domain of the large-subunitribosomal DNA sequence were identified among theC. lusitaniae strains. The greatest degree of polymorphismwas found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% ofthe isolates. This study provides significant insight into thegenetic diversity of the mycobiota present during the henequenfermentation process, especially that of C. lusitaniae,for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealingpolymorphisms between isolates of this species.